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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

COLGALT1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
COLGALT1 GLT25D1
Sequence Length (AA) Molecular Weight (Da)
622 71636
Protein Name
Procollagen galactosyltransferase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAAPRAGRR RGQPLLALLL LLLAPLPPGA PPGADAYFPE ERWSPESPLQ
60 70 80 90 100
APRVLIALLA RNAAHALPTT LGALERLRHP RERTALWVAT DHNMDNTSTV
110 120 130 140 150
LREWLVAVKS LYHSVEWRPA EEPRSYPDEE GPKHWSDSRY EHVMKLRQAA
160 170 180 190 200
LKSARDMWAD YILFVDADNL ILNPDTLSLL IAENKTVVAP MLDSRAAYSN
210 220 230 240 250
FWCGMTSQGY YKRTPAYIPI RKRDRRGCFA VPMVHSTFLI DLRKAASRNL
260 270 280 290 300
AFYPPHPDYT WSFDDIIVFA FSCKQAEVQM YVCNKEEYGF LPVPLRAHST
310 320 330 340 350
LQDEAESFMH VQLEVMVKHP PAEPSRFISA PTKTPDKMGF DEVFMINLRR
360 370 380 390 400
RQDRRERMLR ALQAQEIECR LVEAVDGKAM NTSQVEALGI QMLPGYRDPY
410 420 430 440 450
HGRPLTKGEL GCFLSHYNIW KEVVDRGLQK SLVFEDDLRF EIFFKRRLMN
460 470 480 490 500
LMRDVEREGL DWDLIYVGRK RMQVEHPEKA VPRVRNLVEA DYSYWTLAYV
510 520 530 540 550
ISLQGARKLL AAEPLSKMLP VDEFLPVMFD KHPVSEYKAH FSLRNLHAFS
560 570 580 590 600
VEPLLIYPTH YTGDDGYVSD TETSVVWNNE HVKTDWDRAK SQKMREQQAL
610 620 622
SREAKNSDVL QSPLDSAARD EL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-209 Collapse assay details

Data source: Panorama

Official Gene Symbol
COLGALT1
Peptide Modified Sequence
ALQAQEIEC[+57.0]R
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
369
Peptide - Site of Modification
9
Peptide Start
361
Peptide End
370
CPTAC ID
CPTAC-209
Peptide Molecular Mass
1,216.5870
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 5.9 7.5 11 13.6 14.9 11.1 14.8 16.7 15.6 15 25 15
y6 (1+) 7.4 7.4 9.1 15 16 6.7 16.7 17.6 11.3 15 25 15
y5 (1+) 8.2 6.7 12.1 12.7 6.9 11.3 15.1 9.6 16.6 15 25 15
sum 6.6 6.3 10.2 13.1 12.6 9.2 14.7 14.1 13.7 15 25 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-208 Collapse assay details

Data source: Panorama

Official Gene Symbol
COLGALT1
Peptide Sequence
TPAYIPIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
214
Peptide End
221
CPTAC ID
CPTAC-208
Peptide Molecular Mass
929.5335
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 8.2 7.4 7.3 7.9 9.7 7.5 11.4 12.2 10.5 15 25 15
y5 (1+) 6.9 8.6 6.5 7.2 9.2 7.5 10 12.6 9.9 15 25 15
y4 (1+) 10 11.3 11.7 11.2 10.3 12.7 15 15.3 17.3 15 25 15
sum 6.2 4.8 5.9 6.7 6.8 6.8 9.1 8.3 9 15 25 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1298 Collapse assay details

Data source: Panorama

Official Gene Symbol
COLGALT1
Peptide Modified Sequence
TALWVATDHNM[+16.0]DN[+1.0]TSTVLR
Modification Type
Oxidation (M), Deamidated (N)
Protein - Site of Modification
94, 96
Peptide - Site of Modification
11, 13
Peptide Start
84
Peptide End
102
CPTAC ID
CPTAC-1298
Peptide Molecular Mass
2,161.0110
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y14 (2+) 39.8 8.2 7.3 38.4 9.2 7.8 55.3 12.3 10.7 15 15 15
y15 (2+) 22.4 7.4 5 20.8 8.8 4.5 30.6 11.5 6.7 15 15 15
y6 (1+) 30.4 10.3 8 39.1 11 9.4 49.5 15.1 12.3 15 15 15
sum 22.4 5 4.5 21.7 4.9 4.5 31.2 7 6.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments