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Overview Data source: UniProt

Official Gene Symbol Other Aliases
QPCT N/A
Sequence Length (AA) Molecular Weight (Da)
361 40877
Protein Name
Glutaminyl-peptide cyclotransferase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGGRHRRVV GTLHLLLLVA ALPWASRGVS PSASAWPEEK NYHQPAILNS
60 70 80 90 100
SALRQIAEGT SISEMWQNDL QPLLIERYPG SPGSYAARQH IMQRIQRLQA
110 120 130 140 150
DWVLEIDTFL SQTPYGYRSF SNIISTLNPT AKRHLVLACH YDSKYFSHWN
160 170 180 190 200
NRVFVGATDS AVPCAMMLEL ARALDKKLLS LKTVSDSKPD LSLQLIFFDG
210 220 230 240 250
EEAFLHWSPQ DSLYGSRHLA AKMASTPHPP GARGTSQLHG MDLLVLLDLI
260 270 280 290 300
GAPNPTFPNF FPNSARWFER LQAIEHELHE LGLLKDHSLE GRYFQNYSYG
310 320 330 340 350
GVIQDDHIPF LRRGVPVLHL IPSPFPEVWH TMDDNEENLD ESTIDNLNKI
360 361
LQVFVLEYLH L

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1301 Collapse assay details

Data source: Panorama

Official Gene Symbol
QPCT
Peptide Modified Sequence
YFQN[+1.0]YSYGGVIQDDHIPFLR
Modification Type
Deamidated (N)
Protein - Site of Modification
296
Peptide - Site of Modification
4
Peptide Start
293
Peptide End
312
CPTAC ID
CPTAC-1301
Peptide Molecular Mass
2,432.1437
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 35.2 8.1 15.8 27.7 8 16.3 44.8 11.4 22.7 15 15 15
y6 (1+) 27.6 9.8 9.2 21.7 9.9 9.4 35.1 13.9 13.2 15 15 15
y4 (1+) 9.6 3.5 3.5 11.7 3.1 4.3 15.1 4.7 5.5 15 15 15
sum 10.6 2.2 3.1 11 2.9 3.5 15.3 3.6 4.7 15 15 15

Additional Resources and Comments