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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PCDH1 N/A
Sequence Length (AA) Molecular Weight (Da)
1060 114743
Protein Name
Protocadherin-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDSGAGGRRC PEAALLILGP PRMEHLRHSP GPGGQRLLLP SMLLALLLLL
60 70 80 90 100
APSPGHATRV VYKVPEEQPP NTLIGSLAAD YGFPDVGHLY KLEVGAPYLR
110 120 130 140 150
VDGKTGDIFT TETSIDREGL RECQNQLPGD PCILEFEVSI TDLVQNGSPR
160 170 180 190 200
LLEGQIEVQD INDNTPNFAS PVITLAIPEN TNIGSLFPIP LASDRDAGPN
210 220 230 240 250
GVASYELQAG PEAQELFGLQ VAEDQEEKQP QLIVMGNLDR ERWDSYDLTI
260 270 280 290 300
KVQDGGSPPR ASSALLRVTV LDTNDNAPKF ERPSYEAELS ENSPIGHSVI
310 320 330 340 350
QVKANDSDQG ANAEIEYTFH QAPEVVRRLL RLDRNTGLIT VQGPVDREDL
360 370 380 390 400
STLRFSVLAK DRGTNPKSAR AQVVVTVKDM NDNAPTIEIR GIGLVTHQDG
410 420 430 440 450
MANISEDVAE ETAVALVQVS DRDEGENAAV TCVVAGDVPF QLRQASETGS
460 470 480 490 500
DSKKKYFLQT TTPLDYEKVK DYTIEIVAVD SGNPPLSSTN SLKVQVVDVN
510 520 530 540 550
DNAPVFTQSV TEVAFPENNK PGEVIAEITA SDADSGSNAE LVYSLEPEPA
560 570 580 590 600
AKGLFTISPE TGEIQVKTSL DREQRESYEL KVVAADRGSP SLQGTATVLV
610 620 630 640 650
NVLDCNDNDP KFMLSGYNFS VMENMPALSP VGMVTVIDGD KGENAQVQLS
660 670 680 690 700
VEQDNGDFVI QNGTGTILSS LSFDREQQST YTFQLKAVDG GVPPRSAYVG
710 720 730 740 750
VTINVLDEND NAPYITAPSN TSHKLLTPQT RLGETVSQVA AEDFDSGVNA
760 770 780 790 800
ELIYSIAGGN PYGLFQIGSH SGAITLEKEI ERRHHGLHRL VVKVSDRGKP
810 820 830 840 850
PRYGTALVHL YVNETLANRT LLETLLGHSL DTPLDIDIAG DPEYERSKQR
860 870 880 890 900
GNILFGVVAG VVAVALLIAL AVLVRYCRQR EAKSGYQAGK KETKDLYAPK
910 920 930 940 950
PSGKASKGNK SKGKKSKSPK PVKPVEDEDE AGLQKSLKFN LMSDAPGDSP
960 970 980 990 1000
RIHLPLNYPP GSPDLGRHYR SNSPLPSIQL QPQSPSASKK HQVVQDLPPA
1010 1020 1030 1040 1050
NTFVGTGDTT STGSEQYSDY SYRTNPPKYP SKQVGQPFQL STPQPLPHPY
1060
HGAIWTEVWE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1310 Collapse assay details

Data source: Panorama

Official Gene Symbol
PCDH1
Peptide Modified Sequence
AN[+1.0]DSDQGANAEIEYTFHQAPEVVR
Modification Type
Deamidated (N)
Protein - Site of Modification
305
Peptide - Site of Modification
2
Peptide Start
304
Peptide End
327
CPTAC ID
CPTAC-1310
Peptide Molecular Mass
2,661.1943
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 18.7 7 11 21 7 11.3 28.1 9.9 15.8 15 15 15
y8 (1+) 17.6 9.5 12.5 20.1 9.7 10.9 26.7 13.6 16.6 15 15 15
y7 (1+) 13.3 9.1 7.5 12.4 8 6.9 18.2 12.1 10.2 15 15 15
sum 9.9 4.4 6 11.3 4.7 6 15 6.4 8.5 15 15 15


Additional Resources and Comments