OVGP1, CPTAC-1322 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
OVGP1	MUC9, OGP

Sequence Length (AA)	Molecular Weight (Da)
678	75421

Protein Name
Oviduct-specific glycoprotein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MWKLLLWVGL	VLVLKHHDGA	AHKLVCYFTN	WAHSRPGPAS	ILPHDLDPFL
60	70	80	90	100
CTHLIFAFAS	MNNNQIVAKD	LQDEKILYPE	FNKLKERNRE	LKTLLSIGGW
110	120	130	140	150
NFGTSRFTTM	LSTFANREKF	IASVISLLRT	HDFDGLDLFF	LYPGLRGSPM
160	170	180	190	200
HDRWTFLFLI	EELLFAFRKE	ALLTMRPRLL	LSAAVSGVPH	IVQTSYDVRF
210	220	230	240	250
LGRLLDFINV	LSYDLHGSWE	RFTGHNSPLF	SLPEDPKSSA	YAMNYWRKLG
260	270	280	290	300
APSEKLIMGI	PTYGRTFRLL	KASKNGLQAR	AIGPASPGKY	TKQEGFLAYF
310	320	330	340	350
EICSFVWGAK	KHWIDYQYVP	YANKGKEWVG	YDNAISFSYK	AWFIRREHFG
360	370	380	390	400
GAMVWTLDMD	DVRGTFCGTG	PFPLVYVLND	ILVRAEFSST	SLPQFWLSSA
410	420	430	440	450
VNSSSTDPER	LAVTTAWTTD	SKILPPGGEA	GVTEIHGKCE	NMTITPRGTT
460	470	480	490	500
VTPTKETVSL	GKHTVALGEK	TEITGAMTMT	SVGHQSMTPG	EKALTPVGHQ
510	520	530	540	550
SVTTGQKTLT	SVGYQSVTPG	EKTLTPVGHQ	SVTPVSHQSV	SPGGTTMTPV
560	570	580	590	600
HFQTETLRQN	TVAPRRKAVA	REKVTVPSRN	ISVTPEGQTM	PLRGENLTSE
610	620	630	640	650
VGTHPRMGNL	GLQMEAENRM	MLSSSPVIQL	PEQTPLAFDN	RFVPIYGNHS
660	670	678
SVNSVTPQTS	PLSLKKEIPE	NSAVDEEA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1322 Collapse assay details

Data source: Panorama

Official Gene Symbol: OVGP1
Peptide Modified Sequence: FVPIYGN[+1.0]HSSVNSVTPQTSPLSLK
Modification Type: Deamidated (N)
Protein - Site of Modification: 648
Peptide - Site of Modification: 7
Peptide Start: 642
Peptide End: 665

CPTAC ID: CPTAC-1322
Peptide Molecular Mass: 2,572.3173
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

CPTAC-1297: SUN1. TLSPTGN[+1.0]ISSAPK	CPTAC-1298: COLGALT1. TALWVATDHNM[+16.0]DN[+1.0]TSTVLR	CPTAC-1299: APOB. FN[+1.0]SSYLQGTNQITGR
CPTAC-1300: APOB. FVEGSHN[+1.0]STVSLTTK	CPTAC-1301: QPCT. YFQN[+1.0]YSYGGVIQDDHIPFLR	CPTAC-1302: CA1. EIINVGHSFHVNFEDNDN[+1.0]R
CPTAC-1303: VIM. QDVDN[+1.0]ASLAR	CPTAC-1304: DPP7. N[+1.0]LSASVIAVTIQGGAHHLDLR	CPTAC-1305: SERPING1. M[+16.0]LFVEPILEVSSLPTTN[+1.0]STTNSATK
CPTAC-1306: CEACAM8. LFIPN[+1.0]ITTK	CPTAC-1307: DSG2. ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER	CPTAC-1308: M6PR. LKPLFN[+1.0]K
CPTAC-1309: ACP2. FNPN[+1.0]ISWQPIPVHTVPITEDR	CPTAC-1310: PCDH1. AN[+1.0]DSDQGANAEIEYTFHQAPEVVR	CPTAC-1311: ITGB8. NYAIIKPIGFN[+1.0]ETAK
CPTAC-1312: CALCOCO2. SAN[+1.0]STLAQALHWTR	CPTAC-1313: MINPP1. N[+1.0]ATALYHVEAFK	CPTAC-1314: KRT40. TYN[+1.0]ASVLPVR
CPTAC-1315: MMP23B. ALM[+16.0]HLN[+1.0]ATLR	CPTAC-1316: CD6. EGDN[+1.0]ITLK	CPTAC-1317: CD6. N[+1.0]ATVVWM[+16.0]K
CPTAC-1318: CD6. TVNSLN[+1.0]VSAISIPEHDEADEISDENR	CPTAC-1319: IRAK2. TILVDN[+1.0]NTWNNTHISR	CPTAC-1320: AEN. LN[+1.0]STAQK
CPTAC-1321: PLTP. N[+1.0]WSLPNR	CPTAC-1322: OVGP1. FVPIYGN[+1.0]HSSVNSVTPQTSPLSLK	CPTAC-2612: MMP23B. ALM[+16.0]HLN[+1.0]ATLR

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	7.8	3.8	2.7	8.3	6.5	2.8	11.4	7.5	3.9	15	15	15
y9 (2+)	9.9	6.5	5.8	11	7.2	7.6	14.8	9.7	9.6	15	15	15
y5 (1+)	9.1	3.6	6.4	9.9	4.6	6.8	13.4	5.8	9.3	15	15	15
sum	7.4	2.9	2	7	3.6	3.1	10.2	4.6	3.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1526 Collapse assay details

Data source: Panorama

Official Gene Symbol: OVGP1
Peptide Sequence: ILYPEFNK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 76
Peptide End: 83

CPTAC ID: CPTAC-1526
Peptide Molecular Mass: 1,022.5437
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu