KRT40, CPTAC-1314 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
KRT40	KA36

Sequence Length (AA)	Molecular Weight (Da)
431	48139

Protein Name
Keratin, type I cytoskeletal 40

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTSDCSSTHC	SPESCGTASG	CAPASSCSVE	TACLPGTCAT	SRCQTPSFLS
60	70	80	90	100
RSRGLTGCLL	PCYFTGSCNS	PCLVGNCAWC	EDGVFTSNEK	ETMQFLNDRL
110	120	130	140	150
ASYLEKVRSL	EETNAELESR	IQEQCEQDIP	MVCPDYQRYF	NTIEDLQQKI
160	170	180	190	200
LCTKAENSRL	AVQLDNCKLA	TDDFKSKYES	ELSLRQLLEA	DISSLHGILE
210	220	230	240	250
ELTLCKSDLE	AHVESLKEDL	LCLKKNHEEE	VNLLREQLGD	RLSVELDTAP
260	270	280	290	300
TLDLNRVLDE	MRCQCETVLA	NNRREAEEWL	AVQTEELNQQ	QLSSAEQLQG
310	320	330	340	350
CQMEILELKR	TASALEIELQ	AQQSLTESLE	CTVAETEAQY	SSQLAQIQCL
360	370	380	390	400
IDNLENQLAE	IRCDLERQNQ	EYQVLLDVKA	RLEGEINTYW	GLLDSEDSRL
410	420	430	431
SCSPCSTTCT	SSNTCEPCSA	YVICTVENCC	L

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1314 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRT40
Peptide Modified Sequence: TYN[+1.0]ASVLPVR
Modification Type: Deamidated (N)
Protein - Site of Modification: 3
Peptide - Site of Modification: 3
Peptide Start: 1
Peptide End: 10

CPTAC ID: CPTAC-1314
Peptide Molecular Mass: 1,119.5924
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

CPTAC-1297: SUN1. TLSPTGN[+1.0]ISSAPK	CPTAC-1298: COLGALT1. TALWVATDHNM[+16.0]DN[+1.0]TSTVLR	CPTAC-1299: APOB. FN[+1.0]SSYLQGTNQITGR
CPTAC-1300: APOB. FVEGSHN[+1.0]STVSLTTK	CPTAC-1301: QPCT. YFQN[+1.0]YSYGGVIQDDHIPFLR	CPTAC-1302: CA1. EIINVGHSFHVNFEDNDN[+1.0]R
CPTAC-1303: VIM. QDVDN[+1.0]ASLAR	CPTAC-1304: DPP7. N[+1.0]LSASVIAVTIQGGAHHLDLR	CPTAC-1305: SERPING1. M[+16.0]LFVEPILEVSSLPTTN[+1.0]STTNSATK
CPTAC-1306: CEACAM8. LFIPN[+1.0]ITTK	CPTAC-1307: DSG2. ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER	CPTAC-1308: M6PR. LKPLFN[+1.0]K
CPTAC-1309: ACP2. FNPN[+1.0]ISWQPIPVHTVPITEDR	CPTAC-1310: PCDH1. AN[+1.0]DSDQGANAEIEYTFHQAPEVVR	CPTAC-1311: ITGB8. NYAIIKPIGFN[+1.0]ETAK
CPTAC-1312: CALCOCO2. SAN[+1.0]STLAQALHWTR	CPTAC-1313: MINPP1. N[+1.0]ATALYHVEAFK	CPTAC-1314: KRT40. TYN[+1.0]ASVLPVR
CPTAC-1315: MMP23B. ALM[+16.0]HLN[+1.0]ATLR	CPTAC-1316: CD6. EGDN[+1.0]ITLK	CPTAC-1317: CD6. N[+1.0]ATVVWM[+16.0]K
CPTAC-1318: CD6. TVNSLN[+1.0]VSAISIPEHDEADEISDENR	CPTAC-1319: IRAK2. TILVDN[+1.0]NTWNNTHISR	CPTAC-1320: AEN. LN[+1.0]STAQK
CPTAC-1321: PLTP. N[+1.0]WSLPNR	CPTAC-1322: OVGP1. FVPIYGN[+1.0]HSSVNSVTPQTSPLSLK	CPTAC-2612: MMP23B. ALM[+16.0]HLN[+1.0]ATLR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	27.8	9.9	12.6	29.2	10.2	15.4	40.3	14.2	19.9	15	15	15
y6 (1+)	45.1	14.2	16.1	29.7	13	15.1	54	19.3	22.1	15	15	15
y4 (1+)	67.3	15.1	21	66.1	20.2	17.2	94.3	25.2	27.1	15	15	15
sum	29.2	6.8	9.6	27.2	8	10.1	39.9	10.5	13.9	15	15	15

KRT40

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1314 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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KRT40

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1314 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments