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Overview Data source: UniProt

Official Gene Symbol Other Aliases
KRT40 KA36
Sequence Length (AA) Molecular Weight (Da)
431 48139
Protein Name
Keratin, type I cytoskeletal 40
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTSDCSSTHC SPESCGTASG CAPASSCSVE TACLPGTCAT SRCQTPSFLS
60 70 80 90 100
RSRGLTGCLL PCYFTGSCNS PCLVGNCAWC EDGVFTSNEK ETMQFLNDRL
110 120 130 140 150
ASYLEKVRSL EETNAELESR IQEQCEQDIP MVCPDYQRYF NTIEDLQQKI
160 170 180 190 200
LCTKAENSRL AVQLDNCKLA TDDFKSKYES ELSLRQLLEA DISSLHGILE
210 220 230 240 250
ELTLCKSDLE AHVESLKEDL LCLKKNHEEE VNLLREQLGD RLSVELDTAP
260 270 280 290 300
TLDLNRVLDE MRCQCETVLA NNRREAEEWL AVQTEELNQQ QLSSAEQLQG
310 320 330 340 350
CQMEILELKR TASALEIELQ AQQSLTESLE CTVAETEAQY SSQLAQIQCL
360 370 380 390 400
IDNLENQLAE IRCDLERQNQ EYQVLLDVKA RLEGEINTYW GLLDSEDSRL
410 420 430 431
SCSPCSTTCT SSNTCEPCSA YVICTVENCC L

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1314 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT40
Peptide Modified Sequence
TYN[+1.0]ASVLPVR
Modification Type
Deamidated (N)
Protein - Site of Modification
3
Peptide - Site of Modification
3
Peptide Start
1
Peptide End
10
CPTAC ID
CPTAC-1314
Peptide Molecular Mass
1,119.5924
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 27.8 9.9 12.6 29.2 10.2 15.4 40.3 14.2 19.9 15 15 15
y6 (1+) 45.1 14.2 16.1 29.7 13 15.1 54 19.3 22.1 15 15 15
y4 (1+) 67.3 15.1 21 66.1 20.2 17.2 94.3 25.2 27.1 15 15 15
sum 29.2 6.8 9.6 27.2 8 10.1 39.9 10.5 13.9 15 15 15

Additional Resources and Comments