IRAK2, CPTAC-1319 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IRAK2	N/A

Sequence Length (AA)	Molecular Weight (Da)
625	69433

Protein Name
Interleukin-1 receptor-associated kinase-like 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MACYIYQLPS	WVLDDLCRNM	DALSEWDWME	FASYVITDLT	QLRKIKSMER
60	70	80	90	100
VQGVSITREL	LWWWGMRQAT	VQQLVDLLCR	LELYRAAQII	LNWKPAPEIR
110	120	130	140	150
CPIPAFPDSV	KPEKPLAASV	RKAEDEQEEG	QPVRMATFPG	PGSSPARAHQ
160	170	180	190	200
PAFLQPPEED	APHSLRSDLP	TSSDSKDFST	SIPKQEKLLS	LAGDSLFWSE
210	220	230	240	250
ADVVQATDDF	NQNRKISQGT	FADVYRGHRH	GKPFVFKKLR	ETACSSPGSI
260	270	280	290	300
ERFFQAELQI	CLRCCHPNVL	PVLGFCAARQ	FHSFIYPYMA	NGSLQDRLQG
310	320	330	340	350
QGGSDPLPWP	QRVSICSGLL	CAVEYLHGLE	IIHSNVKSSN	VLLDQNLTPK
360	370	380	390	400
LAHPMAHLCP	VNKRSKYTMM	KTHLLRTSAA	YLPEDFIRVG	QLTKRVDIFS
410	420	430	440	450
CGIVLAEVLT	GIPAMDNNRS	PVYLKDLLLS	DIPSSTASLC	SRKTGVENVM
460	470	480	490	500
AKEICQKYLE	KGAGRLPEDC	AEALATAACL	CLRRRNTSLQ	EVCGSVAAVE
510	520	530	540	550
ERLRGRETLL	PWSGLSEGTG	SSSNTPEETD	DVDNSSLDAS	SSMSVAPWAG
560	570	580	590	600
AATPLLPTEN	GEGRLRVIVG	READSSSEAC	VGLEPPQDVT	ETSWQIEINE
610	620	625
AKRKLMENIL	LYKEEKVDSI	ELFGP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1319 Collapse assay details

Data source: Panorama

Official Gene Symbol: IRAK2
Peptide Modified Sequence: TILVDN[+1.0]NTWNNTHISR
Modification Type: Deamidated (N)
Protein - Site of Modification: 6
Peptide - Site of Modification: 6
Peptide Start: 1
Peptide End: 16

CPTAC ID: CPTAC-1319
Peptide Molecular Mass: 1,897.9282
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

CPTAC-1297: SUN1. TLSPTGN[+1.0]ISSAPK	CPTAC-1298: COLGALT1. TALWVATDHNM[+16.0]DN[+1.0]TSTVLR	CPTAC-1299: APOB. FN[+1.0]SSYLQGTNQITGR
CPTAC-1300: APOB. FVEGSHN[+1.0]STVSLTTK	CPTAC-1301: QPCT. YFQN[+1.0]YSYGGVIQDDHIPFLR	CPTAC-1302: CA1. EIINVGHSFHVNFEDNDN[+1.0]R
CPTAC-1303: VIM. QDVDN[+1.0]ASLAR	CPTAC-1304: DPP7. N[+1.0]LSASVIAVTIQGGAHHLDLR	CPTAC-1305: SERPING1. M[+16.0]LFVEPILEVSSLPTTN[+1.0]STTNSATK
CPTAC-1306: CEACAM8. LFIPN[+1.0]ITTK	CPTAC-1307: DSG2. ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER	CPTAC-1308: M6PR. LKPLFN[+1.0]K
CPTAC-1309: ACP2. FNPN[+1.0]ISWQPIPVHTVPITEDR	CPTAC-1310: PCDH1. AN[+1.0]DSDQGANAEIEYTFHQAPEVVR	CPTAC-1311: ITGB8. NYAIIKPIGFN[+1.0]ETAK
CPTAC-1312: CALCOCO2. SAN[+1.0]STLAQALHWTR	CPTAC-1313: MINPP1. N[+1.0]ATALYHVEAFK	CPTAC-1314: KRT40. TYN[+1.0]ASVLPVR
CPTAC-1315: MMP23B. ALM[+16.0]HLN[+1.0]ATLR	CPTAC-1316: CD6. EGDN[+1.0]ITLK	CPTAC-1317: CD6. N[+1.0]ATVVWM[+16.0]K
CPTAC-1318: CD6. TVNSLN[+1.0]VSAISIPEHDEADEISDENR	CPTAC-1319: IRAK2. TILVDN[+1.0]NTWNNTHISR	CPTAC-1320: AEN. LN[+1.0]STAQK
CPTAC-1321: PLTP. N[+1.0]WSLPNR	CPTAC-1322: OVGP1. FVPIYGN[+1.0]HSSVNSVTPQTSPLSLK	CPTAC-2612: MMP23B. ALM[+16.0]HLN[+1.0]ATLR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	23.8	21	20.4	31.1	21.7	21.1	39.2	30.2	29.3	15	15	15
y14 (2+)	30.2	24.5	25.3	31.8	27.6	27.1	43.9	36.9	37.1	15	15	15
y12 (2+)	33.5	30.5	26.1	31	35.3	39.6	45.6	46.7	47.4	15	15	15
sum	12.8	12.7	18.7	12.8	17.4	13	18.1	21.5	22.8	15	15	15

IRAK2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1319 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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IRAK2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1319 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments