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Overview Data source: UniProt

Official Gene Symbol Other Aliases
IRAK2 N/A
Sequence Length (AA) Molecular Weight (Da)
625 69433
Protein Name
Interleukin-1 receptor-associated kinase-like 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MACYIYQLPS WVLDDLCRNM DALSEWDWME FASYVITDLT QLRKIKSMER
60 70 80 90 100
VQGVSITREL LWWWGMRQAT VQQLVDLLCR LELYRAAQII LNWKPAPEIR
110 120 130 140 150
CPIPAFPDSV KPEKPLAASV RKAEDEQEEG QPVRMATFPG PGSSPARAHQ
160 170 180 190 200
PAFLQPPEED APHSLRSDLP TSSDSKDFST SIPKQEKLLS LAGDSLFWSE
210 220 230 240 250
ADVVQATDDF NQNRKISQGT FADVYRGHRH GKPFVFKKLR ETACSSPGSI
260 270 280 290 300
ERFFQAELQI CLRCCHPNVL PVLGFCAARQ FHSFIYPYMA NGSLQDRLQG
310 320 330 340 350
QGGSDPLPWP QRVSICSGLL CAVEYLHGLE IIHSNVKSSN VLLDQNLTPK
360 370 380 390 400
LAHPMAHLCP VNKRSKYTMM KTHLLRTSAA YLPEDFIRVG QLTKRVDIFS
410 420 430 440 450
CGIVLAEVLT GIPAMDNNRS PVYLKDLLLS DIPSSTASLC SRKTGVENVM
460 470 480 490 500
AKEICQKYLE KGAGRLPEDC AEALATAACL CLRRRNTSLQ EVCGSVAAVE
510 520 530 540 550
ERLRGRETLL PWSGLSEGTG SSSNTPEETD DVDNSSLDAS SSMSVAPWAG
560 570 580 590 600
AATPLLPTEN GEGRLRVIVG READSSSEAC VGLEPPQDVT ETSWQIEINE
610 620 625
AKRKLMENIL LYKEEKVDSI ELFGP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1319 Collapse assay details

Data source: Panorama

Official Gene Symbol
IRAK2
Peptide Modified Sequence
TILVDN[+1.0]NTWNNTHISR
Modification Type
Deamidated (N)
Protein - Site of Modification
6
Peptide - Site of Modification
6
Peptide Start
1
Peptide End
16
CPTAC ID
CPTAC-1319
Peptide Molecular Mass
1,897.9282
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 23.8 21 20.4 31.1 21.7 21.1 39.2 30.2 29.3 15 15 15
y14 (2+) 30.2 24.5 25.3 31.8 27.6 27.1 43.9 36.9 37.1 15 15 15
y12 (2+) 33.5 30.5 26.1 31 35.3 39.6 45.6 46.7 47.4 15 15 15
sum 12.8 12.7 18.7 12.8 17.4 13 18.1 21.5 22.8 15 15 15

Additional Resources and Comments