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Overview Data source: UniProt

Official Gene Symbol Other Aliases
DSG2 CDHF5
Sequence Length (AA) Molecular Weight (Da)
1118 122294
Protein Name
Desmoglein-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MARSPGRAYA LLLLLICFNV GSGLHLQVLS TRNENKLLPK HPHLVRQKRA
60 70 80 90 100
WITAPVALRE GEDLSKKNPI AKIHSDLAEE RGLKITYKYT GKGITEPPFG
110 120 130 140 150
IFVFNKDTGE LNVTSILDRE ETPFFLLTGY ALDARGNNVE KPLELRIKVL
160 170 180 190 200
DINDNEPVFT QDVFVGSVEE LSAAHTLVMK INATDADEPN TLNSKISYRI
210 220 230 240 250
VSLEPAYPPV FYLNKDTGEI YTTSVTLDRE EHSSYTLTVE ARDGNGEVTD
260 270 280 290 300
KPVKQAQVQI RILDVNDNIP VVENKVLEGM VEENQVNVEV TRIKVFDADE
310 320 330 340 350
IGSDNWLANF TFASGNEGGY FHIETDAQTN EGIVTLIKEV DYEEMKNLDF
360 370 380 390 400
SVIVANKAAF HKSIRSKYKP TPIPIKVKVK NVKEGIHFKS SVISIYVSES
410 420 430 440 450
MDRSSKGQII GNFQAFDEDT GLPAHARYVK LEDRDNWISV DSVTSEIKLA
460 470 480 490 500
KLPDFESRYV QNGTYTVKIV AISEDYPRKT ITGTVLINVE DINDNCPTLI
510 520 530 540 550
EPVQTICHDA EYVNVTAEDL DGHPNSGPFS FSVIDKPPGM AEKWKIARQE
560 570 580 590 600
STSVLLQQSE KKLGRSEIQF LISDNQGFSC PEKQVLTLTV CECLHGSGCR
610 620 630 640 650
EAQHDSYVGL GPAAIALMIL AFLLLLLVPL LLLMCHCGKG AKGFTPIPGT
660 670 680 690 700
IEMLHPWNNE GAPPEDKVVP SFLPVDQGGS LVGRNGVGGM AKEATMKGSS
710 720 730 740 750
SASIVKGQHE MSEMDGRWEE HRSLLSGRAT QFTGATGAIM TTETTKTARA
760 770 780 790 800
TGASRDMAGA QAAAVALNEE FLRNYFTDKA ASYTEEDENH TAKDCLLVYS
810 820 830 840 850
QEETESLNAS IGCCSFIEGE LDDRFLDDLG LKFKTLAEVC LGQKIDINKE
860 870 880 890 900
IEQRQKPATE TSMNTASHSL CEQTMVNSEN TYSSGSSFPV PKSLQEANAE
910 920 930 940 950
KVTQEIVTER SVSSRQAQKV ATPLPDPMAS RNVIATETSY VTGSTMPPTT
960 970 980 990 1000
VILGPSQPQS LIVTERVYAP ASTLVDQPYA NEGTVVVTER VIQPHGGGSN
1010 1020 1030 1040 1050
PLEGTQHLQD VPYVMVRERE SFLAPSSGVQ PTLAMPNIAV GQNVTVTERV
1060 1070 1080 1090 1100
LAPASTLQSS YQIPTENSMT ARNTTVSGAG VPGPLPDFGL EESGHSNSTI
1110 1118
TTSSTRVTKH STVQHSYS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1307 Collapse assay details

Data source: Panorama

Official Gene Symbol
DSG2
Peptide Modified Sequence
ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER
Modification Type
Oxidation (M), Deamidated (N)
Protein - Site of Modification
1035, 1043
Peptide - Site of Modification
16, 24
Peptide Start
1020
Peptide End
1049
CPTAC ID
CPTAC-1307
Peptide Molecular Mass
3,129.5652
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 78.4 20.1 18.7 95.1 32.3 26.3 123.3 38 32.3 15 15 15
y9 (1+) 75.3 24.9 19.4 88.6 30.7 29.8 116.3 39.5 35.6 15 15 15
b5 (1+) 108.6 45.7 22.6 158.4 58.4 26.9 192.1 74.2 35.1 15 15 15
sum 68 15.5 12 68.9 14.6 15.2 96.8 21.3 19.4 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3572 Collapse assay details

Data source: Panorama

Official Gene Symbol
Dsg2
Peptide Sequence
ITHQESTSVLLQQSER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
551
Peptide End
566
CPTAC ID
non-CPTAC-3572
Peptide Molecular Mass
1,854.9436
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b10 (2+) 9.8 5.6 6.6 10.1 6 7.4 14.1 8.2 9.9 15 15 15
b9 (2+) 31.2 10.3 7.8 32.9 11.2 9.8 45.3 15.2 12.5 15 15 15
y4 (1+) 10.8 8 6.4 19 8.3 8.6 21.9 11.5 10.7 15 15 15
y5 (1+) 13.6 6.7 5.8 12.3 7.9 6.7 18.3 10.4 8.9 15 15 15
y6 (1+) 17.9 5.8 5.5 11.6 8.9 7.5 21.3 10.6 9.3 15 15 15
sum 6.8 2.6 2.6 6 5.1 5.5 9.1 5.7 6.1 15 15 15

Additional Resources and Comments