DSG2, CPTAC-1307 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DSG2	CDHF5

Sequence Length (AA)	Molecular Weight (Da)
1118	122294

Protein Name
Desmoglein-2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MARSPGRAYA	LLLLLICFNV	GSGLHLQVLS	TRNENKLLPK	HPHLVRQKRA
60	70	80	90	100
WITAPVALRE	GEDLSKKNPI	AKIHSDLAEE	RGLKITYKYT	GKGITEPPFG
110	120	130	140	150
IFVFNKDTGE	LNVTSILDRE	ETPFFLLTGY	ALDARGNNVE	KPLELRIKVL
160	170	180	190	200
DINDNEPVFT	QDVFVGSVEE	LSAAHTLVMK	INATDADEPN	TLNSKISYRI
210	220	230	240	250
VSLEPAYPPV	FYLNKDTGEI	YTTSVTLDRE	EHSSYTLTVE	ARDGNGEVTD
260	270	280	290	300
KPVKQAQVQI	RILDVNDNIP	VVENKVLEGM	VEENQVNVEV	TRIKVFDADE
310	320	330	340	350
IGSDNWLANF	TFASGNEGGY	FHIETDAQTN	EGIVTLIKEV	DYEEMKNLDF
360	370	380	390	400
SVIVANKAAF	HKSIRSKYKP	TPIPIKVKVK	NVKEGIHFKS	SVISIYVSES
410	420	430	440	450
MDRSSKGQII	GNFQAFDEDT	GLPAHARYVK	LEDRDNWISV	DSVTSEIKLA
460	470	480	490	500
KLPDFESRYV	QNGTYTVKIV	AISEDYPRKT	ITGTVLINVE	DINDNCPTLI
510	520	530	540	550
EPVQTICHDA	EYVNVTAEDL	DGHPNSGPFS	FSVIDKPPGM	AEKWKIARQE
560	570	580	590	600
STSVLLQQSE	KKLGRSEIQF	LISDNQGFSC	PEKQVLTLTV	CECLHGSGCR
610	620	630	640	650
EAQHDSYVGL	GPAAIALMIL	AFLLLLLVPL	LLLMCHCGKG	AKGFTPIPGT
660	670	680	690	700
IEMLHPWNNE	GAPPEDKVVP	SFLPVDQGGS	LVGRNGVGGM	AKEATMKGSS
710	720	730	740	750
SASIVKGQHE	MSEMDGRWEE	HRSLLSGRAT	QFTGATGAIM	TTETTKTARA
760	770	780	790	800
TGASRDMAGA	QAAAVALNEE	FLRNYFTDKA	ASYTEEDENH	TAKDCLLVYS
810	820	830	840	850
QEETESLNAS	IGCCSFIEGE	LDDRFLDDLG	LKFKTLAEVC	LGQKIDINKE
860	870	880	890	900
IEQRQKPATE	TSMNTASHSL	CEQTMVNSEN	TYSSGSSFPV	PKSLQEANAE
910	920	930	940	950
KVTQEIVTER	SVSSRQAQKV	ATPLPDPMAS	RNVIATETSY	VTGSTMPPTT
960	970	980	990	1000
VILGPSQPQS	LIVTERVYAP	ASTLVDQPYA	NEGTVVVTER	VIQPHGGGSN
1010	1020	1030	1040	1050
PLEGTQHLQD	VPYVMVRERE	SFLAPSSGVQ	PTLAMPNIAV	GQNVTVTERV
1060	1070	1080	1090	1100
LAPASTLQSS	YQIPTENSMT	ARNTTVSGAG	VPGPLPDFGL	EESGHSNSTI
1110	1118
TTSSTRVTKH	STVQHSYS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1307 Collapse assay details

Data source: Panorama

Official Gene Symbol: DSG2
Peptide Modified Sequence: ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER
Modification Type: Oxidation (M), Deamidated (N)
Protein - Site of Modification: 1035, 1043
Peptide - Site of Modification: 16, 24
Peptide Start: 1020
Peptide End: 1049

CPTAC ID: CPTAC-1307
Peptide Molecular Mass: 3,129.5652
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

CPTAC-1297: SUN1. TLSPTGN[+1.0]ISSAPK	CPTAC-1298: COLGALT1. TALWVATDHNM[+16.0]DN[+1.0]TSTVLR	CPTAC-1299: APOB. FN[+1.0]SSYLQGTNQITGR
CPTAC-1300: APOB. FVEGSHN[+1.0]STVSLTTK	CPTAC-1301: QPCT. YFQN[+1.0]YSYGGVIQDDHIPFLR	CPTAC-1302: CA1. EIINVGHSFHVNFEDNDN[+1.0]R
CPTAC-1303: VIM. QDVDN[+1.0]ASLAR	CPTAC-1304: DPP7. N[+1.0]LSASVIAVTIQGGAHHLDLR	CPTAC-1305: SERPING1. M[+16.0]LFVEPILEVSSLPTTN[+1.0]STTNSATK
CPTAC-1306: CEACAM8. LFIPN[+1.0]ITTK	CPTAC-1307: DSG2. ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER	CPTAC-1308: M6PR. LKPLFN[+1.0]K
CPTAC-1309: ACP2. FNPN[+1.0]ISWQPIPVHTVPITEDR	CPTAC-1310: PCDH1. AN[+1.0]DSDQGANAEIEYTFHQAPEVVR	CPTAC-1311: ITGB8. NYAIIKPIGFN[+1.0]ETAK
CPTAC-1312: CALCOCO2. SAN[+1.0]STLAQALHWTR	CPTAC-1313: MINPP1. N[+1.0]ATALYHVEAFK	CPTAC-1314: KRT40. TYN[+1.0]ASVLPVR
CPTAC-1315: MMP23B. ALM[+16.0]HLN[+1.0]ATLR	CPTAC-1316: CD6. EGDN[+1.0]ITLK	CPTAC-1317: CD6. N[+1.0]ATVVWM[+16.0]K
CPTAC-1318: CD6. TVNSLN[+1.0]VSAISIPEHDEADEISDENR	CPTAC-1319: IRAK2. TILVDN[+1.0]NTWNNTHISR	CPTAC-1320: AEN. LN[+1.0]STAQK
CPTAC-1321: PLTP. N[+1.0]WSLPNR	CPTAC-1322: OVGP1. FVPIYGN[+1.0]HSSVNSVTPQTSPLSLK	CPTAC-2612: MMP23B. ALM[+16.0]HLN[+1.0]ATLR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	78.4	20.1	18.7	95.1	32.3	26.3	123.3	38	32.3	15	15	15
y9 (1+)	75.3	24.9	19.4	88.6	30.7	29.8	116.3	39.5	35.6	15	15	15
b5 (1+)	108.6	45.7	22.6	158.4	58.4	26.9	192.1	74.2	35.1	15	15	15
sum	68	15.5	12	68.9	14.6	15.2	96.8	21.3	19.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3572 Collapse assay details

Data source: Panorama

Official Gene Symbol: Dsg2
Peptide Sequence: ITHQESTSVLLQQSER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 551
Peptide End: 566

CPTAC ID: non-CPTAC-3572
Peptide Molecular Mass: 1,854.9436
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.