Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
VIM N/A
Sequence Length (AA) Molecular Weight (Da)
466 53652
Protein Name
Vimentin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSTRSVSSSS YRRMFGGPGT ASRPSSSRSY VTTSTRTYSL GSALRPSTSR
60 70 80 90 100
SLYASSPGGV YATRSSAVRL RSSVPGVRLL QDSVDFSLAD AINTEFKNTR
110 120 130 140 150
TNEKVELQEL NDRFANYIDK VRFLEQQNKI LLAELEQLKG QGKSRLGDLY
160 170 180 190 200
EEEMRELRRQ VDQLTNDKAR VEVERDNLAE DIMRLREKLQ EEMLQREEAE
210 220 230 240 250
NTLQSFRQDV DNASLARLDL ERKVESLQEE IAFLKKLHEE EIQELQAQIQ
260 270 280 290 300
EQHVQIDVDV SKPDLTAALR DVRQQYESVA AKNLQEAEEW YKSKFADLSE
310 320 330 340 350
AANRNNDALR QAKQESTEYR RQVQSLTCEV DALKGTNESL ERQMREMEEN
360 370 380 390 400
FAVEAANYQD TIGRLQDEIQ NMKEEMARHL REYQDLLNVK MALDIEIATY
410 420 430 440 450
RKLLEGEESR ISLPLPNFSS LNLRETNLDS LPLVDTHSKR TLLIKTVETR
460 466
DGQVINETSQ HHDDLE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1018 Collapse assay details

Data source: Panorama

Official Gene Symbol
VIM
Peptide Modified Sequence
ETNLDS[+80.0]LPLVDTHSK
Modification Type
Phospho (ST)
Protein - Site of Modification
430
Peptide - Site of Modification
6
Peptide Start
425
Peptide End
439
CPTAC ID
CPTAC-1018
Peptide Molecular Mass
1,747.8030
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10-98 (2+) 32.6 13 9.8 43.3 16 12.2 54.2 20.6 15.6 15 24 15
y11-98 (2+) 61.6 11.6 9.1 31.4 12.3 9.3 69.1 16.9 13 15 24 15
y11 (2+) 48.9 12.3 8.8 25.9 11.4 10.3 55.3 16.8 13.5 15 24 15
sum 48.8 11.8 8.1 27.7 11.9 9.5 56.1 16.8 12.5 15 24 15

Additional Resources and Comments


Assay Details for CPTAC-297 Collapse assay details

Data source: Panorama

Official Gene Symbol
VIM
Peptide Sequence
ILLAELEQLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
130
Peptide End
139
CPTAC ID
CPTAC-297
Peptide Molecular Mass
1,168.7067
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 8 7.8 6.9 28.3 16 15.6 29.4 17.8 17.1 15 25 15
y7 (1+) 9.2 6.6 6.2 28.7 13.3 14.7 30.1 14.8 16 15 25 15
y6 (1+) 9.4 7.4 6.2 26.7 13 13.6 28.3 15 14.9 15 25 15
sum 8 6.9 6.3 28 14.3 14.8 29.1 15.9 16.1 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-298 Collapse assay details

Data source: Panorama

Official Gene Symbol
VIM
Peptide Sequence
ISLPLPNFSSLNLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
411
Peptide End
424
CPTAC ID
CPTAC-298
Peptide Molecular Mass
1,569.8879
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 34.2 9.1 5.8 39.2 18.3 15.1 52 20.4 16.2 15 25 15
y9 (1+) 11.7 9.2 5 26 17.8 14.5 28.5 20 15.3 15 25 15
y7 (1+) 18.4 12.9 5 27.5 16.9 14.4 33.1 21.3 15.2 15 25 15
sum 10.6 9.1 4.9 21.5 17.5 14.5 24 19.7 15.3 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-1036 Collapse assay details

Data source: Panorama

Official Gene Symbol
VIM
Peptide Modified Sequence
IS[+80.0]LPLPNFSSLNLR
Modification Type
Phospho (ST)
Protein - Site of Modification
412
Peptide - Site of Modification
2
Peptide Start
411
Peptide End
424
CPTAC ID
CPTAC-1036
Peptide Molecular Mass
1,649.8542
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 51.5 16.7 20.2 46.4 54.5 38.2 69.3 57 43.2 11 19 13
y11 (2+) 50.3 15.6 22.5 39.7 54.2 37 64.1 56.4 43.3 11 19 13
y5 (1+) 47.1 23.7 42.4 37.2 56.3 53.6 60 61.1 68.3 11 19 13
y9 (1+) 45.2 17.6 18.8 27.3 57.4 36.3 52.8 60 40.9 11 19 13
sum 42.1 15.4 20 30.3 55.7 35.4 51.9 57.8 40.7 11 19 13

Additional Resources and Comments


Assay Details for CPTAC-1017 Collapse assay details

Data source: Panorama

Official Gene Symbol
VIM
Peptide Modified Sequence
SLYASS[+80.0]PGGVYATR
Modification Type
Phospho (ST)
Protein - Site of Modification
56
Peptide - Site of Modification
6
Peptide Start
51
Peptide End
64
CPTAC ID
CPTAC-1017
Peptide Molecular Mass
1,507.6708
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 28.1 6.2 7 15.1 8 9.6 31.9 10.1 11.9 15 24 15
y4 (1+) 27.2 6.4 7.1 23.4 7.3 8.5 35.9 9.7 11.1 15 24 15
y7 (1+) 24.6 8.1 7.7 31.4 11.1 7.8 39.9 13.7 11 15 24 15
y8 (1+) 28.4 6.6 7.7 17.5 8.9 9.6 33.4 11.1 12.3 15 24 15
y9 (1+) 41.2 6.5 3.6 32.6 9.2 10.9 52.5 11.3 11.5 15 24 15
sum 25.8 5.9 6.9 14.1 7.6 8.9 29.4 9.6 11.3 15 24 15

Additional Resources and Comments


Assay Details for CPTAC-1303 Collapse assay details

Data source: Panorama

Official Gene Symbol
VIM
Peptide Modified Sequence
QDVDN[+1.0]ASLAR
Modification Type
Deamidated (N)
Protein - Site of Modification
212
Peptide - Site of Modification
5
Peptide Start
208
Peptide End
217
CPTAC ID
CPTAC-1303
Peptide Molecular Mass
1,088.5098
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 55 33.6 18.4 58 32.2 24.1 79.9 46.5 30.3 15 15 15
y6 (1+) 68 39.7 25.3 69 43.4 27.9 96.9 58.8 37.7 15 15 15
y5 (1+) 48.8 19.8 19.3 82.8 24.1 15.7 96.1 31.2 24.9 15 15 15
sum 48.7 14.7 9.6 31.9 16.7 10.2 58.2 22.2 14 15 15 15

Additional Resources and Comments