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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CD6 N/A
Sequence Length (AA) Molecular Weight (Da)
668 71801
Protein Name
T-cell differentiation antigen CD6
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWLFFGITGL LTAALSGHPS PAPPDQLNTS SAESELWEPG ERLPVRLTNG
60 70 80 90 100
SSSCSGTVEV RLEASWEPAC GALWDSRAAE AVCRALGCGG AEAASQLAPP
110 120 130 140 150
TPELPPPPAA GNTSVAANAT LAGAPALLCS GAEWRLCEVV EHACRSDGRR
160 170 180 190 200
ARVTCAENRA LRLVDGGGAC AGRVEMLEHG EWGSVCDDTW DLEDAHVVCR
210 220 230 240 250
QLGCGWAVQA LPGLHFTPGR GPIHRDQVNC SGAEAYLWDC PGLPGQHYCG
260 270 280 290 300
HKEDAGAVCS EHQSWRLTGG ADRCEGQVEV HFRGVWNTVC DSEWYPSEAK
310 320 330 340 350
VLCQSLGCGT AVERPKGLPH SLSGRMYYSC NGEELTLSNC SWRFNNSNLC
360 370 380 390 400
SQSLAARVLC SASRSLHNLS TPEVPASVQT VTIESSVTVK IENKESRELM
410 420 430 440 450
LLIPSIVLGI LLLGSLIFIA FILLRIKGKY ALPVMVNHQH LPTTIPAGSN
460 470 480 490 500
SYQPVPITIP KEVFMLPIQV QAPPPEDSDS GSDSDYEHYD FSAQPPVALT
510 520 530 540 550
TFYNSQRHRV TDEEVQQSRF QMPPLEEGLE ELHASHIPTA NPGHCITDPP
560 570 580 590 600
SLGPQYHPRS NSESSTSSGE DYCNSPKSKL PPWNPQVFSS ERSSFLEQPP
610 620 630 640 650
NLELAGTQPA FSAGPPADDS SSTSSGEWYQ NFQPPPQPPS EEQFGCPGSP
660 668
SPQPDSTDND DYDDISAA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1316 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD6
Peptide Modified Sequence
EGDN[+1.0]ITLK
Modification Type
Deamidated (N)
Protein - Site of Modification
4
Peptide - Site of Modification
4
Peptide Start
1
Peptide End
8
CPTAC ID
CPTAC-1316
Peptide Molecular Mass
889.4393
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 98.8 41.2 32.4 78.9 48.6 33.8 126.4 63.7 46.8 15 15 15
y3 (1+) 62.6 22.2 12 56.2 27.6 15.3 84.1 35.4 19.4 15 15 15
y5 (1+) 107.3 48.7 40 95.7 46.6 31.8 143.8 67.4 51.1 15 15 15
sum 42.6 16.3 11.6 49.8 20.6 12.8 65.5 26.3 17.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1317 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD6
Peptide Modified Sequence
N[+1.0]ATVVWM[+16.0]K
Modification Type
Deamidated (N), Oxidation (M)
Protein - Site of Modification
1, 7
Peptide - Site of Modification
1, 7
Peptide Start
1
Peptide End
8
CPTAC ID
CPTAC-1317
Peptide Molecular Mass
964.4688
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 20.1 16.5 12.5 30 21 15 36.1 26.7 19.5 15 15 15
y4 (1+) 18.6 12.1 9.1 17.1 11.4 10.6 25.3 16.6 14 15 15 15
y6 (1+) 25.8 18.6 15.9 26.7 13.1 18.7 37.1 22.8 24.5 15 15 15
sum 8.2 8.3 8.5 10.1 8.4 10.2 13 11.8 13.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1318 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD6
Peptide Modified Sequence
TVNSLN[+1.0]VSAISIPEHDEADEISDENR
Modification Type
Deamidated (N)
Protein - Site of Modification
6
Peptide - Site of Modification
6
Peptide Start
1
Peptide End
26
CPTAC ID
CPTAC-1318
Peptide Molecular Mass
2,854.3105
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 24.5 16.7 8.7 23.4 22.9 19.9 33.9 28.3 21.7 15 15 15
y9 (1+) 73.2 53.5 23.1 77.7 51.4 27.9 106.7 74.2 36.2 15 15 15
y14 (2+) 32.5 14.8 17.4 32.2 16.5 17.2 45.8 22.2 24.5 15 15 15
sum 21.2 12.4 10.9 20.6 14.1 12.6 29.6 18.8 16.7 15 15 15


Additional Resources and Comments