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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CEACAM8 CGM6
Sequence Length (AA) Molecular Weight (Da)
349 38154
Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 8
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGPISAPSCR WRIPWQGLLL TASLFTFWNP PTTAQLTIEA VPSNAAEGKE
60 70 80 90 100
VLLLVHNLPQ DPRGYNWYKG ETVDANRRII GYVISNQQIT PGPAYSNRET
110 120 130 140 150
IYPNASLLMR NVTRNDTGSY TLQVIKLNLM SEEVTGQFSV HPETPKPSIS
160 170 180 190 200
SNNSNPVEDK DAVAFTCEPE TQNTTYLWWV NGQSLPVSPR LQLSNGNRTL
210 220 230 240 250
TLLSVTRNDV GPYECEIQNP ASANFSDPVT LNVLYGPDAP TISPSDTYYH
260 270 280 290 300
AGVNLNLSCH AASNPPSQYS WSVNGTFQQY TQKLFIPNIT TKNSGSYACH
310 320 330 340 349
TTNSATGRNR TTVRMITVSD ALVQGSSPGL SARATVSIMI GVLARVALI

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1306 Collapse assay details

Data source: Panorama

Official Gene Symbol
CEACAM8
Peptide Modified Sequence
LFIPN[+1.0]ITTK
Modification Type
Deamidated (N)
Protein - Site of Modification
288
Peptide - Site of Modification
5
Peptide Start
284
Peptide End
292
CPTAC ID
CPTAC-1306
Peptide Molecular Mass
1,046.6012
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 15.2 10.1 6.8 14.2 11 6.8 20.8 14.9 9.6 15 15 15
b3 (1+) 25.6 12.8 13.2 32.1 14.9 16.6 41.1 19.6 21.2 15 15 15
y6 (1+) 8.2 5.5 4.7 9.5 6.6 5 12.5 8.6 6.9 15 15 15
sum 6.7 5.4 3.8 6.9 5.1 4.8 9.6 7.4 6.1 15 15 15

Additional Resources and Comments