CEACAM8, CPTAC-5958 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CEACAM8	CGM6

Sequence Length (AA)	Molecular Weight (Da)
349	38154

Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 8

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGPISAPSCR	WRIPWQGLLL	TASLFTFWNP	PTTAQLTIEA	VPSNAAEGKE
60	70	80	90	100
VLLLVHNLPQ	DPRGYNWYKG	ETVDANRRII	GYVISNQQIT	PGPAYSNRET
110	120	130	140	150
IYPNASLLMR	NVTRNDTGSY	TLQVIKLNLM	SEEVTGQFSV	HPETPKPSIS
160	170	180	190	200
SNNSNPVEDK	DAVAFTCEPE	TQNTTYLWWV	NGQSLPVSPR	LQLSNGNRTL
210	220	230	240	250
TLLSVTRNDV	GPYECEIQNP	ASANFSDPVT	LNVLYGPDAP	TISPSDTYYH
260	270	280	290	300
AGVNLNLSCH	AASNPPSQYS	WSVNGTFQQY	TQKLFIPNIT	TKNSGSYACH
310	320	330	340	349
TTNSATGRNR	TTVRMITVSD	ALVQGSSPGL	SARATVSIMI	GVLARVALI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5958 Collapse assay details

Data source: Panorama

Official Gene Symbol: CEACAM8
Peptide Sequence: IIGYVISNQQITPGPAYSNR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 79
Peptide End: 98

CPTAC ID: CPTAC-5958
Peptide Molecular Mass: 2,190.1433
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	11.7	6.2	7.4	17.1	10.3	11.7	20.7	12	13.8	13	25	14
y7 (1+)	10.8	7.2	7.3	12.2	14.4	9.9	16.3	16.1	12.3	13	25	14
y6 (1+)	9.1	8.6	9.9	11.5	11.6	8.9	14.7	14.4	13.3	13	25	14
b5 (1+)	9.2	8.8	6	11.1	10.7	10.6	14.4	13.9	12.2	13	25	14
b6 (1+)	10.6	9.8	4.9	11.4	13.1	8.6	15.6	16.4	9.9	13	25	14
y8 (1+)	7.9	8.4	5.1	6.9	12.8	11.9	10.5	15.3	12.9	13	25	14
sum	5	6.1	4.6	6.6	11	10.5	8.3	12.6	11.5	13	25	14

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for CPTAC-1306 Collapse assay details

Data source: Panorama

Official Gene Symbol: CEACAM8
Peptide Modified Sequence: LFIPN[+1.0]ITTK
Modification Type: Deamidated (N)
Protein - Site of Modification: 288
Peptide - Site of Modification: 5
Peptide Start: 284
Peptide End: 292

CPTAC ID: CPTAC-1306
Peptide Molecular Mass: 1,046.6012
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang