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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPING1 C1IN, C1NH
Sequence Length (AA) Molecular Weight (Da)
500 55154
Protein Name
Plasma protease C1 inhibitor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASRLTLLTL LLLLLAGDRA SSNPNATSSS SQDPESLQDR GEGKVATTVI
60 70 80 90 100
SKMLFVEPIL EVSSLPTTNS TTNSATKITA NTTDEPTTQP TTEPTTQPTI
110 120 130 140 150
QPTQPTTQLP TDSPTQPTTG SFCPGPVTLC SDLESHSTEA VLGDALVDFS
160 170 180 190 200
LKLYHAFSAM KKVETNMAFS PFSIASLLTQ VLLGAGENTK TNLESILSYP
210 220 230 240 250
KDFTCVHQAL KGFTTKGVTS VSQIFHSPDL AIRDTFVNAS RTLYSSSPRV
260 270 280 290 300
LSNNSDANLE LINTWVAKNT NNKISRLLDS LPSDTRLVLL NAIYLSAKWK
310 320 330 340 350
TTFDPKKTRM EPFHFKNSVI KVPMMNSKKY PVAHFIDQTL KAKVGQLQLS
360 370 380 390 400
HNLSLVILVP QNLKHRLEDM EQALSPSVFK AIMEKLEMSK FQPTLLTLPR
410 420 430 440 450
IKVTTSQDML SIMEKLEFFD FSYDLNLCGL TEDPDLQVSA MQHQTVLELT
460 470 480 490 500
ETGVEAAAAS AISVARTLLV FEVQQPFLFV LWDQQHKFPV FMGRVYDPRA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-684 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPING1
Peptide Modified Sequence
DTFVN[+1.0]ASR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
238
Peptide - Site of Modification
5
Peptide Start
234
Peptide End
241
CPTAC ID
CPTAC-684
Peptide Molecular Mass
909.4192
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.8 5.7 3 4.1 3.9 4.8 7.1 6.9 5.7 15 15 15
y5 (1+) 4.2 3.4 3.4 4.1 3.8 4.5 5.9 5.1 5.6 15 15 15
y4 (1+) 4.3 3.9 3.8 5.5 3.8 4.4 7 5.4 5.8 15 15 15
sum 3.1 3.9 3 2.6 3.3 3.9 4 5.1 4.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1305 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPING1
Peptide Modified Sequence
M[+16.0]LFVEPILEVSSLPTTN[+1.0]STTNSATK
Modification Type
Oxidation (M), Deamidated (N)
Protein - Site of Modification
53, 69
Peptide - Site of Modification
1, 17
Peptide Start
53
Peptide End
77
CPTAC ID
CPTAC-1305
Peptide Molecular Mass
2,696.3466
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 23.9 14.1 16.1 23.9 15 19.2 33.8 20.6 25.1 15 15 15
b7 (1+) 19.3 15.4 11 18.9 22.8 16.6 27 27.5 19.9 15 15 15
b9 (1+) 21 32.6 14.6 24 37.2 11.2 31.9 49.5 18.4 15 15 15
sum 13.3 11.8 8.9 15 10.2 11.8 20 15.6 14.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1147 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPING1
Peptide Sequence
FQPTLLTLPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
391
Peptide End
400
CPTAC ID
non-CPTAC-1147
Peptide Molecular Mass
1,184.6917
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 7.2 4.5 4.8 11.5 6.7 5 13.6 8.1 6.9 15 15 15
y4 (1+) 7.3 4.4 5.5 12.4 6.8 6.2 14.4 8.1 8.3 15 15 15
y5 (1+) 7.5 3.1 6.5 13.6 5.2 6.5 15.5 6.1 9.2 15 15 15
y6 (1+) 9.1 2.9 4.8 11.9 6.5 5.8 15 7.1 7.5 15 15 15
y8 (1+) 6.1 4.2 5.7 11 5.1 5.3 12.6 6.6 7.8 15 15 15
sum 4.7 3.1 3.9 10.2 4.9 4 11.2 5.8 5.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1148 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPING1
Peptide Sequence
GVTSVSQIFHSPDLAIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
222
Peptide End
238
CPTAC ID
non-CPTAC-1148
Peptide Molecular Mass
1,825.9686
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y15 (3+) 23.8 6.4 4.8 18.4 10.5 6.2 30.1 12.3 7.8 15 15 15
y12 (2+) 17.3 6.6 2.9 17 9.9 4.9 24.3 11.9 5.7 15 15 15
y13 (2+) 20.7 4.8 6 19.9 10.5 8.2 28.7 11.5 10.2 15 15 15
y14 (2+) 21 8.5 6.8 15.2 9.7 9.4 25.9 12.9 11.6 15 15 15
y15 (2+) 21.6 5.1 4.7 17.2 11.5 5.3 27.6 12.6 7.1 15 15 15
sum 20 4.5 3 16.4 9 5 25.9 10.1 5.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3370 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serping1
Peptide Sequence
GVTSVSQIFHSPDLAIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
222
Peptide End
238
CPTAC ID
non-CPTAC-3370
Peptide Molecular Mass
1,825.9686
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y15 (3+) 3 3.3 4 11.3 7.9 8.5 11.7 8.6 9.4 15 15 15
y12 (2+) 2.8 5 5.4 14.2 5.8 5.7 14.5 7.7 7.9 15 15 15
y13 (2+) 5.9 3.6 4.9 11.7 7.8 7.9 13.1 8.6 9.3 15 15 15
y14 (2+) 5.2 4.3 4.1 9.4 7.5 7.6 10.7 8.6 8.6 15 15 15
y15 (2+) 4.1 2.5 7.1 13 7.6 8.2 13.6 8 10.8 15 15 15
sum 2.4 1.8 3.1 11.8 6.7 5.2 12 6.9 6.1 15 15 15


Additional Resources and Comments