DPP7, CPTAC-1304 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DPP7	DPP2, QPP

Sequence Length (AA)	Molecular Weight (Da)
492	54341

Protein Name
Dipeptidyl peptidase 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGSAPWAPVL	LLALGLRGLQ	AGARRAPDPG	FQERFFQQRL	DHFNFERFGN
60	70	80	90	100
KTFPQRFLVS	DRFWVRGEGP	IFFYTGNEGD	VWAFANNSAF	VAELAAERGA
110	120	130	140	150
LLVFAEHRYY	GKSLPFGAQS	TQRGHTELLT	VEQALADFAE	LLRALRRDLG
160	170	180	190	200
AQDAPAIAFG	GSYGGMLSAY	LRMKYPHLVA	GALAASAPVL	AVAGLGDSNQ
210	220	230	240	250
FFRDVTADFE	GQSPKCTQGV	REAFRQIKDL	FLQGAYDTVR	WEFGTCQPLS
260	270	280	290	300
DEKDLTQLFM	FARNAFTVLA	MMDYPYPTDF	LGPLPANPVK	VGCDRLLSEA
310	320	330	340	350
QRITGLRALA	GLVYNASGSE	HCYDIYRLYH	SCADPTGCGT	GPDARAWDYQ
360	370	380	390	400
ACTEINLTFA	SNNVTDMFPD	LPFTDELRQR	YCLDTWGVWP	RPDWLLTSFW
410	420	430	440	450
GGDLRAASNI	IFSNGNLDPW	AGGGIRRNLS	ASVIAVTIQG	GAHHLDLRAS
460	470	480	490	492
HPEDPASVVE	ARKLEATIIG	EWVKAARREQ	QPALRGGPRL	SL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1304 Collapse assay details

Data source: Panorama

Official Gene Symbol: DPP7
Peptide Modified Sequence: N[+1.0]LSASVIAVTIQGGAHHLDLR
Modification Type: Deamidated (N)
Protein - Site of Modification: 428
Peptide - Site of Modification: 1
Peptide Start: 428
Peptide End: 448

CPTAC ID: CPTAC-1304
Peptide Molecular Mass: 2,172.1651
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

CPTAC-1297: SUN1. TLSPTGN[+1.0]ISSAPK	CPTAC-1298: COLGALT1. TALWVATDHNM[+16.0]DN[+1.0]TSTVLR	CPTAC-1299: APOB. FN[+1.0]SSYLQGTNQITGR
CPTAC-1300: APOB. FVEGSHN[+1.0]STVSLTTK	CPTAC-1301: QPCT. YFQN[+1.0]YSYGGVIQDDHIPFLR	CPTAC-1302: CA1. EIINVGHSFHVNFEDNDN[+1.0]R
CPTAC-1303: VIM. QDVDN[+1.0]ASLAR	CPTAC-1304: DPP7. N[+1.0]LSASVIAVTIQGGAHHLDLR	CPTAC-1305: SERPING1. M[+16.0]LFVEPILEVSSLPTTN[+1.0]STTNSATK
CPTAC-1306: CEACAM8. LFIPN[+1.0]ITTK	CPTAC-1307: DSG2. ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER	CPTAC-1308: M6PR. LKPLFN[+1.0]K
CPTAC-1309: ACP2. FNPN[+1.0]ISWQPIPVHTVPITEDR	CPTAC-1310: PCDH1. AN[+1.0]DSDQGANAEIEYTFHQAPEVVR	CPTAC-1311: ITGB8. NYAIIKPIGFN[+1.0]ETAK
CPTAC-1312: CALCOCO2. SAN[+1.0]STLAQALHWTR	CPTAC-1313: MINPP1. N[+1.0]ATALYHVEAFK	CPTAC-1314: KRT40. TYN[+1.0]ASVLPVR
CPTAC-1315: MMP23B. ALM[+16.0]HLN[+1.0]ATLR	CPTAC-1316: CD6. EGDN[+1.0]ITLK	CPTAC-1317: CD6. N[+1.0]ATVVWM[+16.0]K
CPTAC-1318: CD6. TVNSLN[+1.0]VSAISIPEHDEADEISDENR	CPTAC-1319: IRAK2. TILVDN[+1.0]NTWNNTHISR	CPTAC-1320: AEN. LN[+1.0]STAQK
CPTAC-1321: PLTP. N[+1.0]WSLPNR	CPTAC-1322: OVGP1. FVPIYGN[+1.0]HSSVNSVTPQTSPLSLK	CPTAC-2612: MMP23B. ALM[+16.0]HLN[+1.0]ATLR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	44	17.7	15.1	50.6	28.4	15.8	67.1	33.5	21.9	15	15	15
y14 (2+)	54.8	12.8	19.7	51.7	13.3	20.2	75.3	18.5	28.2	15	15	15
y12 (2+)	75.8	17.9	26.9	78.2	19.8	25.9	108.9	26.7	37.3	15	15	15
sum	33.5	8.5	11.8	31.2	9	10.9	45.8	12.4	16.1	15	15	15

DPP7

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1304 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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DPP7

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1304 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments