ACP2, CPTAC-1309 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ACP2	N/A

Sequence Length (AA)	Molecular Weight (Da)
423	48344

Protein Name
Lysosomal acid phosphatase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGKRSGWSR	AALLQLLLGV	NLVVMPPTRA	RSLRFVTLLY	RHGDRSPVKT
60	70	80	90	100
YPKDPYQEEE	WPQGFGQLTK	EGMLQHWELG	QALRQRYHGF	LNTSYHRQEV
110	120	130	140	150
YVRSTDFDRT	LMSAEANLAG	LFPPNGMQRF	NPNISWQPIP	VHTVPITEDR
160	170	180	190	200
LLKFPLGPCP	RYEQLQNETR	QTPEYQNESS	RNAQFLDMVA	NETGLTDLTL
210	220	230	240	250
ETVWNVYDTL	FCEQTHGLRL	PPWASPQTMQ	RLSRLKDFSF	RFLFGIYQQA
260	270	280	290	300
EKARLQGGVL	LAQIRKNLTL	MATTSQLPKL	LVYSAHDTTL	VALQMALDVY
310	320	330	340	350
NGEQAPYASC	HIFELYQEDS	GNFSVEMYFR	NESDKAPWPL	SLPGCPHRCP
360	370	380	390	400
LQDFLRLTEP	VVPKDWQQEC	QLASGPADTE	VIVALAVCGS	ILFLLIVLLL
410	420	423
TVLFRMQAQP	PGYRHVADGE	DHA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1309 Collapse assay details

Data source: Panorama

Official Gene Symbol: ACP2
Peptide Modified Sequence: FNPN[+1.0]ISWQPIPVHTVPITEDR
Modification Type: Deamidated (N)
Protein - Site of Modification: 133
Peptide - Site of Modification: 4
Peptide Start: 130
Peptide End: 150

CPTAC ID: CPTAC-1309
Peptide Molecular Mass: 2,460.2438
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

CPTAC-1297: SUN1. TLSPTGN[+1.0]ISSAPK	CPTAC-1298: COLGALT1. TALWVATDHNM[+16.0]DN[+1.0]TSTVLR	CPTAC-1299: APOB. FN[+1.0]SSYLQGTNQITGR
CPTAC-1300: APOB. FVEGSHN[+1.0]STVSLTTK	CPTAC-1301: QPCT. YFQN[+1.0]YSYGGVIQDDHIPFLR	CPTAC-1302: CA1. EIINVGHSFHVNFEDNDN[+1.0]R
CPTAC-1303: VIM. QDVDN[+1.0]ASLAR	CPTAC-1304: DPP7. N[+1.0]LSASVIAVTIQGGAHHLDLR	CPTAC-1305: SERPING1. M[+16.0]LFVEPILEVSSLPTTN[+1.0]STTNSATK
CPTAC-1306: CEACAM8. LFIPN[+1.0]ITTK	CPTAC-1307: DSG2. ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER	CPTAC-1308: M6PR. LKPLFN[+1.0]K
CPTAC-1309: ACP2. FNPN[+1.0]ISWQPIPVHTVPITEDR	CPTAC-1310: PCDH1. AN[+1.0]DSDQGANAEIEYTFHQAPEVVR	CPTAC-1311: ITGB8. NYAIIKPIGFN[+1.0]ETAK
CPTAC-1312: CALCOCO2. SAN[+1.0]STLAQALHWTR	CPTAC-1313: MINPP1. N[+1.0]ATALYHVEAFK	CPTAC-1314: KRT40. TYN[+1.0]ASVLPVR
CPTAC-1315: MMP23B. ALM[+16.0]HLN[+1.0]ATLR	CPTAC-1316: CD6. EGDN[+1.0]ITLK	CPTAC-1317: CD6. N[+1.0]ATVVWM[+16.0]K
CPTAC-1318: CD6. TVNSLN[+1.0]VSAISIPEHDEADEISDENR	CPTAC-1319: IRAK2. TILVDN[+1.0]NTWNNTHISR	CPTAC-1320: AEN. LN[+1.0]STAQK
CPTAC-1321: PLTP. N[+1.0]WSLPNR	CPTAC-1322: OVGP1. FVPIYGN[+1.0]HSSVNSVTPQTSPLSLK	CPTAC-2612: MMP23B. ALM[+16.0]HLN[+1.0]ATLR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	9.6	4.4	4.7	10.9	6.2	7.4	14.5	7.6	8.8	15	15	15
y11 (1+)	6.8	4.3	4.9	7.9	5	5.2	10.4	6.6	7.1	15	15	15
y11 (2+)	13.8	7.5	6.5	8.5	14.2	16.7	16.2	16.1	17.9	15	15	15
sum	7.3	2.5	3.3	6.4	5.7	7.8	9.7	6.2	8.5	15	15	15

ACP2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1309 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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ACP2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1309 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments