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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Dsg2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Dsg2 N/A
Sequence Length (AA) Molecular Weight (Da)
1122 122385
Protein Name
Desmoglein-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MARSPGDRCA LLLLVQLLAV VCLDFGNGLH LEVFSPRNEG KPFPKHTHLV
60 70 80 90 100
RQKRAWITAP VALREGEDLS RKNPIAKIHS DLAEEKGIKI TYKYTGKGIT
110 120 130 140 150
EPPFGIFVFD RNTGELNITS ILDREETPYF LLTGYALDSR GNNLEKPLEL
160 170 180 190 200
RIKVLDINDN EPVFTQEVFV GSIEELSAAH TLVMKITATD ADDPETLNAK
210 220 230 240 250
VSYRIVSQEP ANSHMFYLNK DTGEIYTTSF TLDREEHSSY SLTVEARDGN
260 270 280 290 300
GQITDKPVQQ AQVQIRILDV NDNIPVVENK MYEGTVEENQ VNVEVMRIKV
310 320 330 340 350
TDADEVGSDN WLANFTFASG NEGGYFHIET DTQTNEGIVT LVKEVDYEEM
360 370 380 390 400
KKLDLSIIVT NKAAFHKSIL SKYKATPIPI TVKVKNVVEG IHFKSSVVSF
410 420 430 440 450
RASEAMDRSS LSRSIGNFQV FDEDTGQAAK VTYVKVQDTD NWVSVDSVTS
460 470 480 490 500
EIKLVKIPDF ESRYVQNGTY TAKVVAISKE HPQKTITGTI VITVEDVNDN
510 520 530 540 550
CPVLVDSVRS VCEDEPYVNV TAEDLDGAQN SAPFSFSIID QPPGTAQKWK
560 570 580 590 600
ITHQESTSVL LQQSERKRGR SEIPFLISDS QGFSCPERQV LQLTVCECLK
610 620 630 640 650
GGGCVAAQYD NYVGLGPAAI ALMILALLLL LLVPLLLLIC HCGGGAKGFT
660 670 680 690 700
PIPGTIEMLH PWNNEGAPPE DKVVPSLLVA DHAESSAVRG GVGGAMLKEG
710 720 730 740 750
MMKGSSSASV TKGQHELSEV DGRWEEHRSL LTAGATHHVR TAGTIAANEA
760 770 780 790 800
VRTRATGSSR DMSGARGAVA VNEEFLRSYF TEKAASYNGE DDLHMAKDCL
810 820 830 840 850
LVYSQEDTAS LRGSVGCCSF IEGELDDLFL DDLGLKFKTL AEVCLGRKID
860 870 880 890 900
LDVDIEQRQK PVREASVSAA SGSHYEQAVT SSESAYSSNT GFPAPKPLHE
910 920 930 940 950
VHTEKVTQEI VTESSVSSRQ SQKVVPPPDP VASGNIIVTE TSYAKGSAVP
960 970 980 990 1000
PSTVLLAPRQ PQSLIVTERV YAPTSTLVDQ HYANEEKVLV TERVIQPNGG
1010 1020 1030 1040 1050
IPKPLEVTQH LKDAQYVMVR ERESILAPSS GVQPTLAMPS VAAGGQNVTV
1060 1070 1080 1090 1100
TERILTPAST LQSSYQIPSE TSITARNTVL SSVGSIGPLP NLDLEESDRP
1110 1120 1122
NSTITTSSTR VTKHSTMQHS YS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1307 Collapse assay details

Data source: Panorama

Official Gene Symbol
DSG2
Peptide Modified Sequence
ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER
Modification Type
Oxidation (M), Deamidated (N)
Protein - Site of Modification
1035, 1043
Peptide - Site of Modification
16, 24
Peptide Start
1020
Peptide End
1049
CPTAC ID
CPTAC-1307
Peptide Molecular Mass
3,129.5652
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 78.4 20.1 18.7 95.1 32.3 26.3 123.3 38 32.3 15 15 15
y9 (1+) 75.3 24.9 19.4 88.6 30.7 29.8 116.3 39.5 35.6 15 15 15
b5 (1+) 108.6 45.7 22.6 158.4 58.4 26.9 192.1 74.2 35.1 15 15 15
sum 68 15.5 12 68.9 14.6 15.2 96.8 21.3 19.4 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-3572 Collapse assay details

Data source: Panorama

Official Gene Symbol
Dsg2
Peptide Sequence
ITHQESTSVLLQQSER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
551
Peptide End
566
CPTAC ID
non-CPTAC-3572
Peptide Molecular Mass
1,854.9436
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b10 (2+) 9.8 5.6 6.6 10.1 6 7.4 14.1 8.2 9.9 15 15 15
b9 (2+) 31.2 10.3 7.8 32.9 11.2 9.8 45.3 15.2 12.5 15 15 15
y4 (1+) 10.8 8 6.4 19 8.3 8.6 21.9 11.5 10.7 15 15 15
y5 (1+) 13.6 6.7 5.8 12.3 7.9 6.7 18.3 10.4 8.9 15 15 15
y6 (1+) 17.9 5.8 5.5 11.6 8.9 7.5 21.3 10.6 9.3 15 15 15
sum 6.8 2.6 2.6 6 5.1 5.5 9.1 5.7 6.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments