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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Hba Hba-a1
Sequence Length (AA) Molecular Weight (Da)
142 15085
Protein Name
Hemoglobin subunit alpha
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVLSGEDKSN IKAAWGKIGG HGAEYGAEAL ERMFASFPTT KTYFPHFDVS
60 70 80 90 100
HGSAQVKGHG KKVADALASA AGHLDDLPGA LSALSDLHAH KLRVDPVNFK
110 120 130 140 142
LLSHCLLVTL ASHHPADFTP AVHASLDKFL ASVSTVLTSK YR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3573 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hba
Peptide Sequence
TYFPHFDVSHGSAQVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
42
Peptide End
57
CPTAC ID
non-CPTAC-3573
Peptide Molecular Mass
1,818.8689
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (3+) 6.6 3.2 3.3 9.4 12.4 12.1 11.5 12.8 12.5 15 15 15
y13 (3+) 9.1 3 3.8 8.1 10 13.7 12.2 10.4 14.2 15 15 15
y14 (3+) 6 4.4 4.3 6.4 10.7 12.3 8.8 11.6 13 15 15 15
y15 (3+) 10.4 5.8 3.7 10.4 10.3 12.1 14.7 11.8 12.7 15 15 15
y4 (1+) 35.9 40.2 26.8 47.5 67.5 37.5 59.5 78.6 46.1 15 15 15
sum 4.6 2.8 2.9 2.9 10.2 12.1 5.4 10.6 12.4 15 15 15


Additional Resources and Comments