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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ncan Cspg3
Sequence Length (AA) Molecular Weight (Da)
1268 137200
Protein Name
Neurocan core protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGAGSVWASG LLLLWLLLLV AGDQDTQDTT ATEKGLRMLK SGSGPVRAAL
60 70 80 90 100
AELVALPCFF TLQPRLSSLR DIPRIKWTKV QTASGQRQDL PILVAKDNVV
110 120 130 140 150
RVAKGWQGRV SLPAYPRHRA NATLLLGPLR ASDSGLYRCQ VVKGIEDEQD
160 170 180 190 200
LVTLEVTGVV FHYRAARDRY ALTFAEAQEA CRLSSATIAA PRHLQAAFED
210 220 230 240 250
GFDNCDAGWL SDRTVRYPIT QSRPGCYGDR SSLPGVRSYG RRDPQELYDV
260 270 280 290 300
YCFARELGGE VFYVGPARRL TLAGARAQCQ RQGAALASVG QLHLAWHEGL
310 320 330 340 350
DQCDPGWLAD GSVRYPIQTP RRRCGGPAPG VRTVYRFANR TGFPAPGARF
360 370 380 390 400
DAYCFRAHHH TAQHGDSEIP SSGDEGEIVS AEGPPGRELK PSLGEQEVIA
410 420 430 440 450
PDFQEPLMSS GEGEPPDLTW TQAPEETLGS TPGGPTLASW PSSEKWLFTG
460 470 480 490 500
APSSMGVSSP SDMGVDMEAT TPLGTQVAPT PTMRRGRFKG LNGRHFQQQG
510 520 530 540 550
PEDQLPEVAE PSAQPPTLGA TANHMRPSAA TEASESDQSH SPWAILTNEV
560 570 580 590 600
DEPGAGSLGS RSLPESLMWS PSLISPSVPS TESTPSPKPG AAEAPSVKSA
610 620 630 640 650
IPHLPRLPSE PPAPSPGPSE ALSAVSLQAS SADGSPDFPI VAMLRAPKLW
660 670 680 690 700
LLPRSTLVPN MTPVPLSPAS PLPSWVPEEQ AVRPVSLGAE DLETPFQTTI
710 720 730 740 750
AAPVEASHRS PDADSIEIEG TSSMRATKHP ISGPWASLDS SNVTMNPVPS
760 770 780 790 800
DAGILGTESG VLDLPGSPTS GGQATVEKVL ATWLPLPGQG LDPGSQSTPM
810 820 830 840 850
EAHGVAVSME PTVALEGGAT EGPMEATREV VPSTADATWE SESRSAISST
860 870 880 890 900
HIAVTMARAQ GMPTLTSTSS EGHPEPKGQM VAQESLEPLN TLPSHPWSSL
910 920 930 940 950
VVPMDEVASV SSGEPTGLWD IPSTLIPVSL GLDESVLNVV AESPSVEGFW
960 970 980 990 1000
EEVASGQEDP TDPCENNPCL HGGTCHTNGT VYGCSCDQGY AGENCEIDID
1010 1020 1030 1040 1050
DCLCSPCENG GTCIDEVNGF ICLCLPSYGG SLCEKDTEGC DRGWHKFQGH
1060 1070 1080 1090 1100
CYRYFAHRRA WEDAERDCRR RAGHLTSVHS PEEHKFINSF GHENSWIGLN
1110 1120 1130 1140 1150
DRTVERDFQW TDNTGLQYEN WREKQPDNFF AGGEDCVVMV AHESGRWNDV
1160 1170 1180 1190 1200
PCNYNLPYVC KKGTVLCGPP PAVENASLVG VRKIKYNVHA TVRYQCDEGF
1210 1220 1230 1240 1250
SQHRVATIRC RNNGKWDRPQ IMCIKPRRSH RMRRHHHHPH RHHKPRKEHR
1260 1268
KHKRHPAEDW EKDEGDFC

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3484 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ncan
Peptide Sequence
ELGGEVFYVGPAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
256
Peptide End
268
CPTAC ID
non-CPTAC-3484
Peptide Molecular Mass
1,392.7038
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 26.6 44.1 62.8 43.4 59.6 58.1 50.9 74.1 85.6 15 15 15
y5 (1+) 5.3 4.2 5.6 7.4 7.2 7.8 9.1 8.3 9.6 15 15 15
y6 (1+) 7.1 5 3.4 8 4.8 4.1 10.7 6.9 5.3 15 15 15
y7 (1+) 6.9 3.6 5.7 10.2 7 9.8 12.3 7.9 11.3 15 15 15
y8 (1+) 10.1 4.8 3 12.3 7.9 4.8 15.9 9.2 5.7 15 15 15
sum 3.3 1.6 2 5.8 5.1 4.8 6.7 5.3 5.2 15 15 15


Additional Resources and Comments