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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Notch3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Notch3 N/A
Sequence Length (AA) Molecular Weight (Da)
2318 244248
Protein Name
Neurogenic locus notch homolog protein 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGLGARGRRR RRRLMALPPP PPPMRALPLL LLLAGLGAAA PPCLDGSPCA
60 70 80 90 100
NGGRCTHQQP SLEAACLCLP GWVGERCQLE DPCHSGPCAG RGVCQSSVVA
110 120 130 140 150
GTARFSCRCL RGFQGPDCSQ PDPCVSRPCV HGAPCSVGPD GRFACACPPG
160 170 180 190 200
YQGQSCQSDI DECRSGTTCR HGGTCLNTPG SFRCQCPLGY TGLLCENPVV
210 220 230 240 250
PCAPSPCRNG GTCRQSSDVT YDCACLPGFE GQNCEVNVDD CPGHRCLNGG
260 270 280 290 300
TCVDGVNTYN CQCPPEWTGQ FCTEDVDECQ LQPNACHNGG TCFNLLGGHS
310 320 330 340 350
CVCVNGWTGE SCSQNIDDCA TAVCFHGATC HDRVASFYCA CPMGKTGLLC
360 370 380 390 400
HLDDACVSNP CHEDAICDTN PVSGRAICTC PPGFTGGACD QDVDECSIGA
410 420 430 440 450
NPCEHLGRCV NTQGSFLCQC GRGYTGPRCE TDVNECLSGP CRNQATCLDR
460 470 480 490 500
IGQFTCICMA GFTGTYCEVD IDECQSSPCV NGGVCKDRVN GFSCTCPSGF
510 520 530 540 550
SGSMCQLDVD ECASTPCRNG AKCVDQPDGY ECRCAEGFEG TLCERNVDDC
560 570 580 590 600
SPDPCHHGRC VDGIASFSCA CAPGYTGIRC ESQVDECRSQ PCRYGGKCLD
610 620 630 640 650
LVDKYLCRCP PGTTGVNCEV NIDDCASNPC TFGVCRDGIN RYDCVCQPGF
660 670 680 690 700
TGPLCNVEIN ECASSPCGEG GSCVDGENGF HCLCPPGSLP PLCLPANHPC
710 720 730 740 750
AHKPCSHGVC HDAPGGFRCV CEPGWSGPRC SQSLAPDACE SQPCQAGGTC
760 770 780 790 800
TSDGIGFRCT CAPGFQGHQC EVLSPCTPSL CEHGGHCESD PDRLTVCSCP
810 820 830 840 850
PGWQGPRCQQ DVDECAGASP CGPHGTCTNL PGNFRCICHR GYTGPFCDQD
860 870 880 890 900
IDDCDPNPCL HGGSCQDGVG SFSCSCLDGF AGPRCARDVD ECLSSPCGPG
910 920 930 940 950
TCTDHVASFT CACPPGYGGF HCEIDLPDCS PSSCFNGGTC VDGVSSFSCL
960 970 980 990 1000
CRPGYTGTHC QYEADPCFSR PCLHGGICNP THPGFECTCR EGFTGSQCQN
1010 1020 1030 1040 1050
PVDWCSQAPC QNGGRCVQTG AYCICPPGWS GRLCDIQSLP CTEAAAQMGV
1060 1070 1080 1090 1100
RLEQLCQEGG KCIDKGRSHY CVCPEGRTGS HCEHEVDPCT AQPCQHGGTC
1110 1120 1130 1140 1150
RGYMGGYVCE CPAGYAGDSC EDNIDECASQ PCQNGGSCID LVARYLCSCP
1160 1170 1180 1190 1200
PGTLGVLCEI NEDDCDLGPS LDSGVQCLHN GTCVDLVGGF RCNCPPGYTG
1210 1220 1230 1240 1250
LHCEADINEC RPGACHAAHT RDCLQDPGGH FRCVCHPGFT GPRCQIALSP
1260 1270 1280 1290 1300
CESQPCQHGG QCRHSLGRGG GLTFTCHCVP PFWGLRCERV ARSCRELQCP
1310 1320 1330 1340 1350
VGIPCQQTAR GPRCACPPGL SGPSCRVSRA SPSGATNASC ASAPCLHGGS
1360 1370 1380 1390 1400
CLPVQSVPFF RCVCAPGWGG PRCETPSAAP EVPEEPRCPR AACQAKRGDQ
1410 1420 1430 1440 1450
NCDRECNTPG CGWDGGDCSL NVDDPWRQCE ALQCWRLFNN SRCDPACSSP
1460 1470 1480 1490 1500
ACLYDNFDCY SGGRDRTCNP VYEKYCADHF ADGRCDQGCN TEECGWDGLD
1510 1520 1530 1540 1550
CASEVPALLA RGVLVLTVLL PPEELLRSSA DFLQRLSAIL RTSLRFRLDA
1560 1570 1580 1590 1600
RGQAMVFPYH RPSPGSESRV RRELGPEVIG SVVMLEIDNR LCLQSAENDH
1610 1620 1630 1640 1650
CFPDAQSAAD YLGALSAVER LDFPYPLRDV RGEPLEAPEQ SVPLLPLLVA
1660 1670 1680 1690 1700
GAVFLLIIFI LGVMVARRKR EHSTLWFPEG FALHKDIAAG HKGRREPVGQ
1710 1720 1730 1740 1750
DALGMKNMAK GESLMGEVVT DLNDSECPEA KRLKVEEPGM GAEEPEDCRQ
1760 1770 1780 1790 1800
WTQHHLVAAD IRVAPATALT PPQGDADADG VDVNVRGPDG FTPLMLASFC
1810 1820 1830 1840 1850
GGALEPMPAE EDEADDTSAS IISDLICQGA QLGARTDRTG ETALHLAARY
1860 1870 1880 1890 1900
ARADAAKRLL DAGADTNAQD HSGRTPLHTA VTADAQGVFQ ILIRNRSTDL
1910 1920 1930 1940 1950
DARMADGSTA LILAARLAVE GMVEELIASH ADVNAVDELG KSALHWAAAV
1960 1970 1980 1990 2000
NNVEATLALL KNGANKDMQD SKEETPLFLA AREGSYEAAK LLLDHLANRE
2010 2020 2030 2040 2050
ITDHLDRLPR DVAQERLHQD IVRLLDQPSG PRSPSGPHGL GPLLCPPGAF
2060 2070 2080 2090 2100
LPGLKAVQSG TKKSRRPPGK TGLGPQGTRG RGKKLTLACP GPLADSSVTL
2110 2120 2130 2140 2150
SPVDSLDSPR PFSGPPASPG GFPLEGPYAT TATAVSLAQL GASRAGPLGR
2160 2170 2180 2190 2200
QPPGGCVLSF GLLNPVAVPL DWARLPPPAP PGPSFLLPLA PGPQLLNPGA
2210 2220 2230 2240 2250
PVSPQERPPP YLAAPGHGEE YPAAGTRSSP TKARFLRVPS EHPYLTPSPE
2260 2270 2280 2290 2300
SPEHWASPSP PSLSDWSDST PSPATATNAT ASGALPAQPH PISVPSLPQS
2310 2318
QTQLGPQPEV TPKRQVMA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3485 Collapse assay details

Data source: Panorama

Official Gene Symbol
Notch3
Peptide Sequence
LLDAGADTNAQDHSGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1859
Peptide End
1874
CPTAC ID
non-CPTAC-3485
Peptide Molecular Mass
1,639.7550
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 15.9 5.6 3.2 14 9.1 7.6 21.2 10.7 8.2 15 15 15
y14 (2+) 8.4 4.3 6.1 7.1 4.5 6 11 6.2 8.6 15 15 15
y12 (2+) 9.1 3.9 4.6 9.5 4.1 5.5 13.2 5.7 7.2 15 15 15
b2 (1+) 8 3.5 5.4 9.7 5.3 5.4 12.6 6.4 7.6 15 15 15
b3 (1+) 7.8 4.8 6.2 11.8 7.9 7.5 14.1 9.2 9.7 15 15 15
sum 4.3 2.8 4.3 5.4 4 4.9 6.9 4.9 6.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments