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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Nrp2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Nrp2 N/A
Sequence Length (AA) Molecular Weight (Da)
931 104631
Protein Name
Neuropilin-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDMFPLTWVF LALYFSGHEV RSQQDPPCGG RLNSKDAGYI TSPGYPQDYP
60 70 80 90 100
SHQNCEWIVY APEPNQKIVL NFNPHFEIEK HDCKYDFIEI RDGDSESADL
110 120 130 140 150
LGKHCGNIAP PTIISSGSVL YIKFTSDYAR QGAGFSLRYE IFKTGSEDCS
160 170 180 190 200
KNFTSPNGTI ESPGFPEKYP HNLDCTFTIL AKPRMEIILQ FLTFDLEHDP
210 220 230 240 250
LQVGEGDCKY DWLDIWDGIP HVGPLIGKYC GTKTPSKLRS STGILSLTFH
260 270 280 290 300
TDMAVAKDGF SARYYLIHQE PPENFQCNVP LGMESGRIAN EQISASSTFS
310 320 330 340 350
DGRWTPQQSR LHGDDNGWTP NLDSNKEYLQ VDLRFLTMLT AIATQGAISR
360 370 380 390 400
ETQKGYYVKS YKLEVSTNGE DWMVYRHGKN HKIFQANNDA TEVVLNKLHM
410 420 430 440 450
PLLTRFIRIR PQTWHLGIAL RLELFGCRVT DAPCSNMLGM LSGLIADTQI
460 470 480 490 500
SASSTREYLW SPSAARLVSS RSGWFPRNPQ AQPGEEWLQV DLGTPKTVKG
510 520 530 540 550
VIIQGARGGD SITAVEARAF VRKFKVSYSL NGKDWEYIQD PRTQQTKLFE
560 570 580 590 600
GNMHYDTPDI RRFDPVPAQY VRVYPERWSP AGIGMRLEVL GCDWTDSKPT
610 620 630 640 650
VETLGPTVKS EETTTPYPMD EDATECGENC SFEDDKDLQL PSGFNCNFDF
660 670 680 690 700
PEETCGWVYD HAKWLRSTWI SSANPNDRTF PDDKNFLKLQ SDGRREGQYG
710 720 730 740 750
RLISPPVHLP RSPVCMEFQY QAMGGHGVAL QVVREASQES KLLWVIREDQ
760 770 780 790 800
GSEWKHGRII LPSYDMEYQI VFEGVIGKGR SGEISIDDIR ISTDVPLENC
810 820 830 840 850
MEPISAFAGE DFKVDIPETH GGEGYEDEID DEYEGDWSNS SSSTSGAGDP
860 870 880 890 900
SSGKEKSWLY TLDPILITII AMSSLGVLLG ATCAGLLLYC TCSYSGLSSR
910 920 930 931
SCTTLENYNF ELYDGLKHKV KINHQKCCSE A

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3486 Collapse assay details

Data source: Panorama

Official Gene Symbol
Nrp2
Peptide Sequence
GGDSITAVEAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
508
Peptide End
518
CPTAC ID
non-CPTAC-3486
Peptide Molecular Mass
1,074.5306
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 33.6 8.9 18.3 32 11 19.5 46.4 14.1 26.7 15 15 15
y2 (1+) 27.8 8.5 8.2 26.6 9.2 8.7 38.5 12.5 12 15 15 15
y4 (1+) 24.8 6.2 8.6 26.8 7.6 6.5 36.5 9.8 10.8 15 15 15
y5 (1+) 12.5 5.6 3.6 18.8 8.2 5.8 22.6 9.9 6.8 15 15 15
y6 (1+) 6 3.1 5.8 9.9 5.4 7 11.6 6.2 9.1 15 15 15
sum 5 2.3 3.6 11.3 5.2 4.7 12.4 5.7 5.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments