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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Npy N/A
Sequence Length (AA) Molecular Weight (Da)
97 10874
Protein Name
Pro-neuropeptide Y
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLGNKRMGLC GLTLALSLLV CLGILAEGYP SKPDNPGEDA PAEDMARYYS
60 70 80 90 97
ALRHYINLIT RQRYGKRSSP ETLISDLLMK ESTENAPRTR LEDPSMW

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1290 Collapse assay details

Data source: Panorama

Official Gene Symbol
NPY
Peptide Sequence
HYINLITR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
54
Peptide End
61
CPTAC ID
CPTAC-1290
Peptide Molecular Mass
1,028.5767
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 50 12.8 16.2 68 13.7 18.4 84.4 18.7 24.5 15 15 15
y4 (1+) 46.3 12.3 14.6 49 14 15.9 67.4 18.6 21.6 15 15 15
y6 (1+) 12.5 3.2 2.3 16.1 3.6 5.3 20.4 4.8 5.8 15 15 15
sum 10.9 3 1.9 15.6 3.6 4.8 19 4.7 5.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1288 Collapse assay details

Data source: Panorama

Official Gene Symbol
NPY
Peptide Sequence
SKPDNPGEDAPAEDMAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
31
Peptide End
47
CPTAC ID
CPTAC-1288
Peptide Molecular Mass
1,798.7792
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 29.1 13.9 13.1 25.7 12.4 12.5 38.8 18.6 18.1 15 15 15
y6 (1+) 21.1 8.5 8.9 23.1 8.6 10.6 31.3 12.1 13.8 15 15 15
y7 (1+) 17.3 5.3 6 24.9 5.7 5.5 30.3 7.8 8.1 15 15 15
sum 16 3.7 5.7 21.8 3.8 4.9 27 5.3 7.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1289 Collapse assay details

Data source: Panorama

Official Gene Symbol
NPY
Peptide Modified Sequence
SKPDNPGEDAPAEDM[+16.0]AR
Modification Type
Oxidation (M)
Protein - Site of Modification
45
Peptide - Site of Modification
15
Peptide Start
31
Peptide End
47
CPTAC ID
CPTAC-1289
Peptide Molecular Mass
1,814.7741
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 24.3 7.7 7.2 35.6 7 7.9 43.1 10.4 10.7 15 15 15
y6 (1+) 13.3 7.8 8.6 17.3 10.8 11.7 21.8 13.3 14.5 15 15 15
y7 (1+) 19.3 7.8 7.6 24.6 7.1 7.5 31.3 10.5 10.7 15 15 15
sum 15.8 3.7 5.8 21.7 5.6 6 26.8 6.7 8.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1291 Collapse assay details

Data source: Panorama

Official Gene Symbol
NPY
Peptide Sequence
SSPETLISDLLMR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
68
Peptide End
80
CPTAC ID
CPTAC-1291
Peptide Molecular Mass
1,460.7545
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 49.8 7.9 4.8 44.6 6.8 4.6 66.9 10.4 6.6 15 15 15
y7 (1+) 46.2 8 7.8 50.1 8.3 7.8 68.2 11.5 11 15 15 15
y9 (1+) 65 25.8 21.5 87.4 23.7 22.9 108.9 35 31.4 15 15 15
sum 37.5 5.2 5.2 33.9 5.2 4.9 50.6 7.4 7.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1287 Collapse assay details

Data source: Panorama

Official Gene Symbol
NPY
Peptide Sequence
YPSKPDNPGEDAPAEDMAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
29
Peptide End
47
CPTAC ID
CPTAC-1287
Peptide Molecular Mass
2,058.8953
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 13.8 7.8 6.7 19.5 9 8 23.9 11.9 10.4 15 15 15
y6 (1+) 77.7 21 16.8 91 20.1 16.5 119.7 29.1 23.5 15 15 15
y7 (1+) 16.9 7.4 8.4 18.3 7.7 8 24.9 10.7 11.6 15 15 15
sum 14.5 5.7 4.2 19.8 5.6 4.3 24.5 8 6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3487 Collapse assay details

Data source: Panorama

Official Gene Symbol
Npy
Peptide Sequence
HYINLITR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
54
Peptide End
61
CPTAC ID
non-CPTAC-3487
Peptide Molecular Mass
1,028.5767
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4.1 4.5 2.7 6.2 6.1 6.7 7.4 7.6 7.2 15 15 15
y3 (2+) 47.2 39.9 68.1 54.4 39.4 84.3 72 56.1 108.4 15 15 15
y5 (1+) 6.9 3 5.1 9 6.9 5.3 11.3 7.5 7.4 15 15 15
y6 (1+) 5.2 3.1 2.9 9.5 6.1 5.5 10.8 6.8 6.2 15 15 15
y7 (1+) 7.1 3.9 4.2 12.9 9.5 10 14.7 10.3 10.8 15 15 15
sum 3.4 2.1 1.7 8 6.6 4.7 8.7 6.9 5 15 15 15


Additional Resources and Comments