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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Epx

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Epx Eper
Sequence Length (AA) Molecular Weight (Da)
716 81380
Protein Name
Eosinophil peroxidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMQQLLALVG ALATLILTQH AEGTAPASPS PVEISVLRDC IAEAKLLVDT
60 70 80 90 100
AYNHTQKSIM QRLRSGSASP MDLLAYFKQP VAATRRVVQA ADYMHVALGL
110 120 130 140 150
LEERLQPRGS RPFNATDVLT EPQLRLLSQA SGCALQDQAE RCSNKYRTIT
160 170 180 190 200
GRCNNKKHPW LGASNQALAR WLPAEYEDHR SLPFGWTPGK RRNGFLLPLV
210 220 230 240 250
RDVSNQIVRF PSKKLTSDRG RALMFMQWGQ FIDHDLDFSP ESPARVAFSM
260 270 280 290 300
GVDCEKTCAQ LPPCFPIKIP RNDPRIKNQR DCIPFFRSAP ACPQNRNKVR
310 320 330 340 350
NQINALTSFV DASMVYGSEV TLALRLRNRT NFLGLLATNQ RFQDNGRALL
360 370 380 390 400
PFDNLHEDPC LLTNRSARIP CFLAGDTRSS ETPKLTALHT LFVREHNRLA
410 420 430 440 450
AELRRLNPHW SGDKLYNEAR KIVGAMVQII TYRDFLPLVL GRARIRRTLG
460 470 480 490 500
PYRGYCSNVD PRVANVFTLA FRFGHTMLQP FMFRLDSQYR ASAPNSHVPL
510 520 530 540 550
SSVFFASWRI IHEGGIDPIL RGLMATPAKL NRQDSMLVDE LRDKLFQQVR
560 570 580 590 600
RIGLDLAALN MQRSRDHGLP GYNAWRRFCG LSQPRNLAQL SRVLKNQDLA
610 620 630 640 650
RKFLRLYKTP DNIDIWVGAI AEPLLPGARV GPLLACLFEN QFRRARDGDR
660 670 680 690 700
FWWQKWGVFT KRQRKALRRI SLSRIVCDNT GITTVSRDIF RANIYPQGFV
710 716
SCSRIPKLNL SAWRGK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3488 Collapse assay details

Data source: Panorama

Official Gene Symbol
Epx
Peptide Sequence
DVSNQIVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
202
Peptide End
209
CPTAC ID
non-CPTAC-3488
Peptide Molecular Mass
929.4930
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.6 5.5 4 16.8 15.7 12.1 17.7 16.6 12.7 15 15 15
y4 (1+) 19.7 6.7 6.1 30.8 17.4 14.2 36.6 18.6 15.5 15 15 15
y6 (2+) 14.3 8.6 7.6 26.4 17.3 14.8 30 19.3 16.6 15 15 15
b2 (1+) 11.5 7.2 5.7 22.3 15.3 15.3 25.1 16.9 16.3 15 15 15
b4 (2+) 136.4 136.4 87.8 113.1 179.9 102.6 177.2 225.8 135 15 15 15
sum 4.4 3.3 2 18.8 15.1 12.6 19.3 15.5 12.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments