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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Penk Penk1
Sequence Length (AA) Molecular Weight (Da)
268 31004
Protein Name
Proenkephalin-A
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MARFLRLCTW LLALGSCLLA TVQAECSQDC AKCSYRLVRP GDINFLACTL
60 70 80 90 100
ECEGQLPSFK IWETCKDLLQ VSRPEFPWDN IDMYKDSSKQ DESHLLAKKY
110 120 130 140 150
GGFMKRYGGF MKKMDELYPM EPEEEANGGE ILAKRYGGFM KKDADEGDTL
160 170 180 190 200
ANSSDLLKEL LGTGDNRAKD SHQQESTNND EDMSKRYGGF MRSLKRSPQL
210 220 230 240 250
EDEAKELQKR YGGFMRRVGR PEWWMDYQKR YGGFLKRFAE SLPSDEEGEN
260 268
YSKEVPEIEK RYGGFMRF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3489 Collapse assay details

Data source: Panorama

Official Gene Symbol
Penk
Peptide Sequence
ELLGTGDNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
159
Peptide End
167
CPTAC ID
non-CPTAC-3489
Peptide Molecular Mass
973.4829
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 8.6 7.4 5.8 14.5 9 6.2 16.9 11.7 8.5 15 15 15
y6 (1+) 6.9 4.7 5.7 11.5 7.6 5.6 13.4 8.9 8 15 15 15
y4 (1+) 32.9 4.4 2.1 70.9 10.3 3.3 78.2 11.2 3.9 15 15 15
y2 (1+) 8.4 5.3 6.8 12.2 7.1 6.2 14.8 8.9 9.2 15 15 15
b2 (1+) 7.2 5.3 7.6 16.5 8.4 7 18 9.9 10.3 15 15 15
sum 14.1 2.4 1.9 29.1 6.4 1.8 32.3 6.8 2.6 15 15 15

Additional Resources and Comments