Prtn3, CPTAC-3494 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Prtn3	N/A

Sequence Length (AA)	Molecular Weight (Da)
254	27626

Protein Name
Myeloblastin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSGSYPSPKG	IHPFLLLALV	VGGAVQASKI	VGGHEARPHS	RPYVASLQLS
60	70	80	90	100
RFPGSHFCGG	TLIHPRFVLT	AAHCLQDISW	QLVTVVLGAH	DLLSSEPEQQ
110	120	130	140	150
KFTISQVFQN	NYNPEENLND	VLLLQLNRTA	SLGKEVAVAS	LPQQDQTLSQ
160	170	180	190	200
GTQCLAMGWG	RLGTQAPTPR	VLQELNVTVV	TFLCREHNVC	TLVPRRAAGI
210	220	230	240	250
CFGDSGGPLI	CNGILHGVDS	FVIRECASLQ	FPDFFARVSM	YVDWIQNVLR
254
GAEP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3494 Collapse assay details

Data source: Panorama

Official Gene Symbol: Prtn3
Peptide Sequence: LGTQAPTPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 162
Peptide End: 170

CPTAC ID: non-CPTAC-3494
Peptide Molecular Mass: 939.5138
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (2+)	20.1	7.8	8.6	19.1	11.3	10.4	27.7	13.7	13.5	15	15	15
y2 (1+)	38.2	10.9	6.4	37.9	13	6.4	53.8	17	9.1	15	15	15
y5 (1+)	9.4	4.6	6.3	11.5	5.7	5.1	14.9	7.3	8.1	15	15	15
y6 (1+)	22.2	2.8	8.2	25.5	9	8	33.8	9.4	11.5	15	15	15
y8 (1+)	20.7	5	7.2	21.5	9.2	10.5	29.8	10.5	12.7	15	15	15
sum	6.3	3.1	3.9	7.7	5.1	3.6	9.9	6	5.3	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-1142 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRTN3
Peptide Sequence: LVNVVLGAHNVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 80
Peptide End: 91

CPTAC ID: non-CPTAC-1142
Peptide Molecular Mass: 1,289.7568
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers