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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Wdr81

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Wdr81 N/A
Sequence Length (AA) Molecular Weight (Da)
1934 211945
Protein Name
WD repeat-containing protein 81
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAQGSRRRKV VLTAGSEGWS PSSGPDMEEL LRSVERDLNI DARQLALAPG
60 70 80 90 100
GTHVVALVST RWLASLRERR LGPCPRAEGL GEAEVRTLLQ RSVQRLPPGW
110 120 130 140 150
TRVEVHGLRK RRLSYPLGGG VPFEEGSCSP ETLTRFMQEV AAQNYRNLWR
160 170 180 190 200
HAYHTYGQPY SHSTAPSALP ALDSIRQALQ RVYGCTFLPV GESIPCLSNV
210 220 230 240 250
RDGPCPSRGS PACPSLLRAE ALLESPEMLY VVHPYVQFSL HDVVTFSPAK
260 270 280 290 300
LTNSQAKVLF LLFRVLRAMD ACHRQGLACG ALSLHHIAVD EKLCSELRLD
310 320 330 340 350
LSAYEMPSED ENQEGSEEKN GTGIKSEKEG EGRTECPTCQ KELRGLVLDW
360 370 380 390 400
VHGRISNFHY LMQLNRLAGR RQGDPNYHPV LPWVVDFTTP YGRFRDLRKS
410 420 430 440 450
KFRLNKGDKQ LDFTYEMTRQ AFVAGGAGSG EPPHVPHHIS DVLSDITYYV
460 470 480 490 500
YKARRTPRSV LCGHVRAQWE PHEYPATMER MQTWTPDECI PEFYTDPSIF
510 520 530 540 550
CSIHPDMPDL DVPAWCSSNQ EFVAAHRALL ESWEVSQDLH HWIDLTFGYK
560 570 580 590 600
LQGKEAVKEK NVCLHLVDAH THLTSYGVVQ LFDQPHPQRL AGSPALAPEP
610 620 630 640 650
PLIPRLLVQP IREATGQEDI SGQLINGAGR LVVEATPCET GWTRDRPGTG
660 670 680 690 700
EDDLEQATEA LDSISLPGKA GDQPGSSSSQ ASPGLLSFSA PSGSRPGRRS
710 720 730 740 750
KAAGLDPGEG EEGKIVLPEG FSPIQALEEL EKVGNFLAKG LGSQLEEPEK
760 770 780 790 800
PHAQPPVHLQ SLFHRDMQVL GVLLAEMVFA TRVRILQPDA PLWVRFEAVR
810 820 830 840 850
GLCIRHSKDI PVSLQPVLDT LLQLSGPKSP MVSKKGKLDP LFEYRPVSQG
860 870 880 890 900
LPPPSPAQLL SPFSSVVPFP PYFPALHKFI LLYQARRVED EVQGRELAFA
910 920 930 940 950
LWQQLGAVLN DITPEGLEIL LPFVLSLMSE EHTAVYTAWY LFEPVAKALG
960 970 980 990 1000
PKNANKYLLK PLIGAYESPC RLHGRFYLYT DCFVAQLVVR LGLQAFLTHL
1010 1020 1030 1040 1050
LPHVLQVLAG VEASQEEGKG LVGTTEDEES ELPVSGPGSC AFGEEIQMDG
1060 1070 1080 1090 1100
QPAASSGLGL PDYRSGVSFH DQADLPDTED FQAGLYVAES PQPQEAEAVS
1110 1120 1130 1140 1150
LGQLSDKSST SEASQGEERG GDDGGAPADK NSVKSGDSSQ DLKQSEGSEE
1160 1170 1180 1190 1200
EEEEEGCVVL EEDQEDEVTG TSELTLSDTM LSMETVVAPG DGRDREEEEE
1210 1220 1230 1240 1250
PLTEQTEGKE QKILLDTACK MVRWLSAKLG PTVASRHVAR NLLRLLTSCY
1260 1270 1280 1290 1300
VGPTRQQFTV SSDDTPPLNA GNIYQKRPVL GDIVSGPVLS CLLHIAYLYG
1310 1320 1330 1340 1350
EPVLTYQYLP YISYLVAPGS NSNPSRLNSR KEAGLLAAVT LTQKIIVYLS
1360 1370 1380 1390 1400
DTTLMDILPR ISHEVLLPVL GFLTSFVTGF PSGAQARTVL CVKTISLIAL
1410 1420 1430 1440 1450
ICLRIGQEMV QQHLSEPVAT FFQVFSHLHE LRQQDLPLDP KGCTEGQLPE
1460 1470 1480 1490 1500
ATFSDGQRRP VDPTLLEELQ KVFTLEMAYT IYVPFSCLLG DIIRKIIPNH
1510 1520 1530 1540 1550
ELVGELAGLY LESMSPSSRN PASMEPTMAS AGPEWDPQSG SCLQDDGHSG
1560 1570 1580 1590 1600
TFGSVLVGNR IQIPDSQPQS PGPLGSLSGV GSSGGLSNRN EDNALKRELP
1610 1620 1630 1640 1650
RSAHGLSGNW LAYWQYEIGV SQQDAHFHFH QIRLQSFPGH TGAVKCVAAL
1660 1670 1680 1690 1700
SSEDFFLSGS KDRTVRLWPL YNYGDGTNET ASRLIYAQHR KSVFYVGQLE
1710 1720 1730 1740 1750
APQYVVSCDG AVHVWDPFTG KTLRTVDPSD SRVPLTAVAV MPAPHTSITM
1760 1770 1780 1790 1800
ASSDSTLRFV DCRKPGLQHE FRLGGGLNPG LVRSLAVSPS GRSVVAGFSS
1810 1820 1830 1840 1850
GFMVLLDTRT GLVLRGWPAH EGDILQIKAV EGSVLISSSS DHSLTVWKEL
1860 1870 1880 1890 1900
EQKPTHHYKS ASDPIHTFDL YGSEVVTGTV ANKIGVCSLL EPPSQATTKL
1910 1920 1930 1934
SSENFRGTLT SLALLPTKRH LLLGSDNGII RLLA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3495 Collapse assay details

Data source: Panorama

Official Gene Symbol
Wdr81
Peptide Sequence
LGPTVASR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1229
Peptide End
1236
CPTAC ID
non-CPTAC-3495
Peptide Molecular Mass
799.4552
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 5.2 4 2.4 10.7 4.3 3.1 11.9 5.9 3.9 15 15 15
y3 (1+) 16.5 5.1 4.8 26.7 8.2 5.8 31.4 9.7 7.5 15 15 15
y4 (1+) 9.1 4.7 2.6 14.4 8.4 6.9 17 9.6 7.4 15 15 15
y5 (1+) 8.8 6 6.4 13 9.5 7.2 15.7 11.2 9.6 15 15 15
y7 (1+) 5 6.6 11.7 6.8 7 73.6 8.4 9.6 74.5 15 15 15
sum 4.3 3.6 1.8 9 4.6 5.7 10 5.8 6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments