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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Chgb Scg-1, Scg1
Sequence Length (AA) Molecular Weight (Da)
677 77969
Protein Name
Secretogranin-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQPAMLLGLL GAAALAAVSS APVDNRDHNE EMVTRCIIEV LSNALSKSSV
60 70 80 90 100
PTITPECRQV LKKSGKEVKG EEKGENQNSK FEVRLLRDPA DASGTRWASS
110 120 130 140 150
REDAGAPVED SQGQTKVGNE KWTEGGGHSR EGVDDQESLR PSNQQASKEA
160 170 180 190 200
KIYHSEERVG KEREKEEGKI YPMGEHREDA GEEKKHIEDS GEKPNTFSNK
210 220 230 240 250
RSEASAKKKD ESVARADAHS MELEEKTHSR EQSSQESGEE TRRQEKPQEL
260 270 280 290 300
TDQDQSQEES QEGEEGEEGE EGEEGEEDSA SEVTKRRPRH HHGRSGSNKS
310 320 330 340 350
SYEGHPLSEE RRPSPKESKE ADVATVRLGE KRSHHLAHYR ASEEEPEYGE
360 370 380 390 400
ESRSYRGLQY RGRGSEEDRA PRPRSEESQE REYKRNHPDS ELESTANRHG
410 420 430 440 450
EETEEERSYE GANGRQHRGR GREPGAHSAL DTREEKRLLD EGHYPVRESP
460 470 480 490 500
IDTAKRYPQS KWQEQEKNYL NYGEEGDQGR WWQQEEQLGP EESREEVRFP
510 520 530 540 550
DRQYEPYPIT EKRKRLGALF NPYFDPLQWK NSDFEKRGNP DDSFLEDEGE
560 570 580 590 600
DRNGVTLTEK NSFPEYNYDW WERRPFSEDV NWGYEKRSFA RAPQLDLKRQ
610 620 630 640 650
YDGVAELDQL LHYRKKADEF PDFYDSEEQM GPHQEANDEK ARADQRVLTA
660 670 677
EEKKELENLA AMDLELQKIA EKFSQRG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3496 Collapse assay details

Data source: Panorama

Official Gene Symbol
Chgb
Peptide Sequence
LLDEGHYPVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
438
Peptide End
447
CPTAC ID
non-CPTAC-3496
Peptide Molecular Mass
1,197.6142
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) NA NA NA NA NA NA NA NA NA 0 0 0
y8 (2+) 3.8 3.3 2.9 7 4.7 4.3 8 5.7 5.2 15 15 15
y9 (2+) 4.8 4.1 4.7 8.4 5.4 4.8 9.7 6.8 6.7 15 15 15
y3 (1+) 8.9 3.5 4.3 9.9 5.7 4.7 13.3 6.7 6.4 15 15 15
y4 (1+) 7.5 3.1 4.1 9.7 5.9 5.5 12.3 6.7 6.9 15 15 15
sum 2.3 2.2 1.6 6.6 4.5 3.5 7 5 3.8 15 15 15


Additional Resources and Comments