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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Sipa1l2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Sipa1l2 Kiaa0545
Sequence Length (AA) Molecular Weight (Da)
1722 189413
Protein Name
Signal-induced proliferation-associated 1-like protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSDPRPSQAE KHKLGRAAAK LKDPSRTMQA DDYFARKFKA INGSMGPATL
60 70 80 90 100
NTSSSSEGGG GGGGPANGTP AVPKMGVRAR VSEWPPKKDC SKDLACKTLW
110 120 130 140 150
ESRSQSSYES VTSIIQNGQN DQGDRQPEEQ LDLDFVEAKY TIGDIFVHSP
160 170 180 190 200
QRGLHPIRQR SNSDITISDI DTEDVLDQHA VNPNTGAALH REYGSTSSID
210 220 230 240 250
RQGLSGENVF AMLRGYRIES YDPKVTGSFG FPDFFPCDTA ISPSLHAAAQ
260 270 280 290 300
ISRGEFVRIS GLDYMDGGLL MGRDRDKPFK RRLKSESVET SLFRKLRAVK
310 320 330 340 350
SEHETFKFTS DLEEGRLDRG IRPWSCQRCF AHYDVQSILF NINEAMATRA
360 370 380 390 400
SVGKRKNITT GASAASQTPV PVGPAGGCES PLGSKEDLNS KENPDADEGD
410 420 430 440 450
GKSNDLVLSC PYFRNETGGE GDRRIALSRA NSASFSSGES CSFESSLSSH
460 470 480 490 500
CTNAGVSVLE VPRESQPIHR EKVKRYIIEH VDLGAYYYRK FFYGKEHQNY
510 520 530 540 550
FGIDENLGPV AVSIRREKVE DPREKEGSQF NYRVAFRTSE LTTLRGAILE
560 570 580 590 600
DAVPSTARHG TARGLPLKEV LEYVIPELSI QCLRQAANSP KVPEQLLKLD
610 620 630 640 650
EQGLSFQHKI GILYCRAGQS TEEEMYNNET AGPAFEEFLD LLGQRVRLKG
660 670 680 690 700
FSKYRAQLDN KTDSTGTHSL YTTYKDFELM FHVSTLLPYM PNNRQQLLRK
710 720 730 740 750
RHIGNDIVTI VFQEPGALPF TPKNIRSHFQ HVFVIVKVHN PCTENVCYSV
760 770 780 790 800
GVSRSKDVPP FGPPIPKGVT FPKSAVFRDF LLAKVINAEN AAHKSEKFRA
810 820 830 840 850
MATRTRQEYL KDLAENFVTT ATVDTSAKFS FITLGAKKKE RVKPRKDAHL
860 870 880 890 900
FSIGAIMWHV VARDFGQSAD IECLLGISNE FIMLIEKDSK NVVFNCSCRD
910 920 930 940 950
VIGWTSGLVS IKAFYERGEC LLLSSVDNRS EDIREIVQRL LIVTRGCETV
960 970 980 990 1000
EMTLRRNGLG QLGFHVNFEG IVADVEPFGF AWKAGLRQGS RLVEICKVAV
1010 1020 1030 1040 1050
ATLTHEQMID LLRTSVTVKV VIIQPHEDGS PRRGCSELCR IPMVEYKLDS
1060 1070 1080 1090 1100
EGTPCEYKTP FRRNTTWHRV PTPALQPVSR ASPVPGTPDR LQCQPLLQQA
1110 1120 1130 1140 1150
QAAIPRSTSF DRKLPDGTRS SPSNQSSSSD PGPGGSGPWR PQVGYDGCPS
1160 1170 1180 1190 1200
PLLLEHQGPG SVECDGTGEQ EDLLEGGRLP ETKWHGPPSK VLSSYKERVL
1210 1220 1230 1240 1250
QKDGSCKESP NKLSHIGDKS CSSHSSSNTL SSNTSSNSDD KHFGSGDLMD
1260 1270 1280 1290 1300
PELLGLTYIK GASTDSGIDT TPCMPATILG PVHLTGSRSL MHSRAEQWAD
1310 1320 1330 1340 1350
AADVSVADDD PAKMYALHGY ASAISSSAAD GSMGDLSEVS SHSSGSQHSG
1360 1370 1380 1390 1400
SPSAHCSKST GSLDSSKVYI VTHGGGQQAP GAVTKPYHRQ GAANKYVIGW
1410 1420 1430 1440 1450
KKSEGSPPPE EPEVTECPRI YGEMDIMSTA TQHPAVVGDS VSETQHVLSK
1460 1470 1480 1490 1500
DDFLKLMLPD SPLVEEGRRK FSFYGNVSPR RSLYRTLSDE SVCSNRRGSS
1510 1520 1530 1540 1550
FASSRSSILE QALPNDILFS TTPPYHSTLP PRTHPAPSMG SLRNEFWFSD
1560 1570 1580 1590 1600
GSLSDKSKCA DPGLMPLPDT AAGLDWSHLV DAARAFEGLD SDEELGLLCH
1610 1620 1630 1640 1650
HASYLDQRVA SFCTLTDLQH GQELEGAPEL SLCVDPTSGK EFMDTPGERS
1660 1670 1680 1690 1700
PSTLTGKVNQ LELILRQLQT DLRKEKQDKA VLQAEVQHLR QDNMRLQEES
1710 1720 1722
QTATAQLRKF TEWFFSTIDK KA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3497 Collapse assay details

Data source: Panorama

Official Gene Symbol
Sipa1l2
Peptide Sequence
GAILEDAVPSTAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
546
Peptide End
558
CPTAC ID
non-CPTAC-3497
Peptide Molecular Mass
1,298.6830
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b8 (1+) 4.6 4.4 4.9 9.3 4.5 5.6 10.4 6.3 7.4 15 15 15
y5 (1+) 4.4 2.9 2.6 11 3.9 3.1 11.8 4.9 4 15 15 15
y7 (1+) 11.1 6.1 4.7 15.9 6 6 19.4 8.6 7.6 15 15 15
y8 (1+) 13.5 3.6 3.2 17.9 6.8 3.6 22.4 7.7 4.8 15 15 15
y9 (1+) 8.4 2.7 5 14.7 7.4 5.8 16.9 7.9 7.7 15 15 15
sum 3.7 1.9 2.1 9.8 3.6 3.2 10.5 4.1 3.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments