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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Scarf2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Scarf2 Srec2
Sequence Length (AA) Molecular Weight (Da)
833 87871
Protein Name
Scavenger receptor class F member 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEGAGSRGAG PARRQGARGL GLLLLLWLLP GLAAPQDLNP RGRNVCRTPG
60 70 80 90 100
SQVLTCCAGW RQLGDECGIA VCEGNSTCSE NEVCVRPGEC RCRHGYFGAN
110 120 130 140 150
CDTKCPRQFW GPDCKERCSC HPHGQCEDVT GQCTCHARRW GARCEHACQC
160 170 180 190 200
QHGTCHPRSG ACRCEPGWWG AQCASACYCS ATSRCDPQTG ACLCHVGWWG
210 220 230 240 250
RSCNNQCACN SSPCEQQSGR CQCRERMFGA RCDRYCQCSH GRCHPVDGTC
260 270 280 290 300
ACDPGYRGKY CREPCPAGFY GPGCRRRCGQ CKGQQPCTVV EGRCLTCEPG
310 320 330 340 350
WNGTKCDQPC ATGFYGEGCG HRCPPCRDGH ACNHVTGKCT HCNAGWIGDR
360 370 380 390 400
CETKCSNGTY GEDCAFVCSD CGSGHCDFQS GRCLCSPGVH GPHCNVTCPA
410 420 430 440 450
GLHGVDCAQA CSCHEESCDP VTGACHLETN QRKGVMGAGA LLTLLLGLLL
460 470 480 490 500
SLLGCCCACR GKDSARRELT LGRKKAPQRF CGSFSRISMK LPRIPLRRQK
510 520 530 540 550
LPKVVVAHHD LDNTLNCSFL DPPSGLEQPS PSWSSRASFS SFDTTDEGPV
560 570 580 590 600
YCVPHEEATA DSRDLEATAA LTEVAAVSLE PTGTSTPGEE AAVLPASSDS
610 620 630 640 650
ERSASSVEGP SGALYARVAR REARPARTRN EAGGLSLSPS PERRKPPPPD
660 670 680 690 700
PATKPKVSWI HGKHSAAAAA PSPPPAGRKA APSPSGRKRT PSNSSVQPPG
710 720 730 740 750
LTEEAPGPAS PTPPRARARG RGLGLSEPTD AGGPPRSAPE AASMLAAELR
760 770 780 790 800
DKTRSLGRAE KPPPPQKAKR SVLPAATVRT ASASEASGSE KAAASAPAPE
810 820 830 833
TPRKKTPIQK PPRKKSREAA GEPSRAGTAP GAS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3498 Collapse assay details

Data source: Panorama

Official Gene Symbol
Scarf2
Peptide Sequence
SVLPAATVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
771
Peptide End
779
CPTAC ID
non-CPTAC-3498
Peptide Molecular Mass
912.5393
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 16.8 7.8 5.9 17.6 8.1 5.4 24.3 11.2 8 15 15 15
y6 (1+) 7.8 3.6 3.6 6.6 5.6 4.4 10.2 6.7 5.7 15 15 15
y5 (1+) 24 12.1 11.6 27.2 13.8 10.5 36.3 18.4 15.6 15 15 15
y6 (2+) 10.1 5.9 3.6 12.5 5.8 3.7 16.1 8.3 5.2 15 15 15
b3 (1+) 12.7 5.3 5.8 11.5 6.4 6.9 17.1 8.3 9 15 15 15
sum 4.4 2.2 2.2 6.6 3.5 3.3 7.9 4.1 4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments