Vnn1, CPTAC-3504 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Vnn1	N/A

Sequence Length (AA)	Molecular Weight (Da)
512	57091

Protein Name
Pantetheinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGMSWWLACA	AAFSALCVLK	ASSLDTFLAA	VYEHAVILPK	DTLLPVSHSE
60	70	80	90	100
ALALMNQNLD	LLEGAIVSAA	KQGAHIIVTP	EDGIYGVRFT	RDTIYPYLEE
110	120	130	140	150
IPDPQVNWIP	CDNPKRFGST	PVQERLSCLA	KNNSIYVVAN	MGDKKPCNTS
160	170	180	190	200
DSHCPPDGRF	QYNTDVVFDS	QGKLVARYHK	QNIFMGEDQF	NVPMEPEFVT
210	220	230	240	250
FDTPFGKFGV	FTCFDILFHD	PAVTLVTEFQ	VDTILFPTAW	MDVLPHLAAI
260	270	280	290	300
EFHSAWAMGM	GVNFLAANLH	NPSRRMTGSG	IYAPDSPRVF	HYDRKTQEGK
310	320	330	340	350
LLFAQLKSHP	IHSPVNWTSY	ASSVESTPTK	TQEFQSIVFF	DEFTFVELKG
360	370	380	390	400
IKGNYTVCQN	DLCCHLSYQM	SEKRADEVYA	FGAFDGLHTV	EGQYYLQICI
410	420	430	440	450
LLKCKTTNLR	TCGSSVDTAF	TRFEMFSLSG	TFGTRYVFPE	VLLSEVKLAP
460	470	480	490	500
GEFQVSSDGR	LVSLKPTSGP	VLTIGLFGRL	YGKDWASNAS	SDFIAHSLII
510	512
MLIVTPIIHY	LC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-3504 Collapse assay details

Data source: Panorama

Official Gene Symbol: Vnn1
Peptide Sequence: LAPGEFQVSSDGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 448
Peptide End: 460

CPTAC ID: non-CPTAC-3504
Peptide Molecular Mass: 1,361.6575
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (2+)	4.6	6.2	3.5	9.6	7.4	8.6	10.6	9.7	9.3	15	15	15
y12 (2+)	10.2	13.9	7.7	17.5	14.5	6.3	20.3	20.1	9.9	15	15	15
y2 (1+)	12.1	5.8	3.6	14.6	11.9	7.7	19	13.2	8.5	15	15	15
y5 (1+)	8.7	11.3	6.2	19.9	8.3	9.8	21.7	14	11.6	15	15	15
y8 (1+)	19.1	6.4	8.4	24.8	14.3	9	31.3	15.7	12.3	15	15	15
sum	4.1	5.7	3.1	10	7.2	8.2	10.8	9.2	8.8	15	15	15

Vnn1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3504 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

Vnn1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3504 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments