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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Vnn1 N/A
Sequence Length (AA) Molecular Weight (Da)
512 57091
Protein Name
Pantetheinase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGMSWWLACA AAFSALCVLK ASSLDTFLAA VYEHAVILPK DTLLPVSHSE
60 70 80 90 100
ALALMNQNLD LLEGAIVSAA KQGAHIIVTP EDGIYGVRFT RDTIYPYLEE
110 120 130 140 150
IPDPQVNWIP CDNPKRFGST PVQERLSCLA KNNSIYVVAN MGDKKPCNTS
160 170 180 190 200
DSHCPPDGRF QYNTDVVFDS QGKLVARYHK QNIFMGEDQF NVPMEPEFVT
210 220 230 240 250
FDTPFGKFGV FTCFDILFHD PAVTLVTEFQ VDTILFPTAW MDVLPHLAAI
260 270 280 290 300
EFHSAWAMGM GVNFLAANLH NPSRRMTGSG IYAPDSPRVF HYDRKTQEGK
310 320 330 340 350
LLFAQLKSHP IHSPVNWTSY ASSVESTPTK TQEFQSIVFF DEFTFVELKG
360 370 380 390 400
IKGNYTVCQN DLCCHLSYQM SEKRADEVYA FGAFDGLHTV EGQYYLQICI
410 420 430 440 450
LLKCKTTNLR TCGSSVDTAF TRFEMFSLSG TFGTRYVFPE VLLSEVKLAP
460 470 480 490 500
GEFQVSSDGR LVSLKPTSGP VLTIGLFGRL YGKDWASNAS SDFIAHSLII
510 512
MLIVTPIIHY LC

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3504 Collapse assay details

Data source: Panorama

Official Gene Symbol
Vnn1
Peptide Sequence
LAPGEFQVSSDGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
448
Peptide End
460
CPTAC ID
non-CPTAC-3504
Peptide Molecular Mass
1,361.6575
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 4.6 6.2 3.5 9.6 7.4 8.6 10.6 9.7 9.3 15 15 15
y12 (2+) 10.2 13.9 7.7 17.5 14.5 6.3 20.3 20.1 9.9 15 15 15
y2 (1+) 12.1 5.8 3.6 14.6 11.9 7.7 19 13.2 8.5 15 15 15
y5 (1+) 8.7 11.3 6.2 19.9 8.3 9.8 21.7 14 11.6 15 15 15
y8 (1+) 19.1 6.4 8.4 24.8 14.3 9 31.3 15.7 12.3 15 15 15
sum 4.1 5.7 3.1 10 7.2 8.2 10.8 9.2 8.8 15 15 15


Additional Resources and Comments