Timp1, CPTAC-3503 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Timp1	Timp, Timp-1

Sequence Length (AA)	Molecular Weight (Da)
205	22628

Protein Name
Metalloproteinase inhibitor 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MMAPFASLAS	GILLLLSLIA	SSKACSCAPP	HPQTAFCNSD	LVIRAKFMGS
60	70	80	90	100
PEINETTLYQ	RYKIKMTKML	KGFKAVGNAA	DIRYAYTPVM	ESLCGYAHKS
110	120	130	140	150
QNRSEEFLIT	GRLRNGNLHI	SACSFLVPWR	TLSPAQQRAF	SKTYSAGCGV
160	170	180	190	200
CTVFPCLSIP	CKLESDTHCL	WTDQVLVGSE	DYQSRHFACL	PRNPGLCTWR
205
SLGAR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-680 Collapse assay details

Data source: Panorama

Official Gene Symbol: TIMP1
Peptide Modified Sequence: FVGTPEVN[+1.0]QTTLYQR
Modification Type: Deamidated (NQ)
Protein - Site of Modification: 53
Peptide - Site of Modification: 8
Peptide Start: 46
Peptide End: 60

CPTAC ID: CPTAC-680
Peptide Molecular Mass: 1,752.8683
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive (ThermoFisher)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing: Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions: 300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	7.7	6.6	9.1	8.2	9.5	9.6	11.2	11.6	13.2	15	15	15
y9 (1+)	13.1	12.8	8.4	14	11.6	11.6	19.2	17.3	14.3	15	15	15
y8 (1+)	7.9	13.1	9.5	7.7	12.5	10.3	11	18.1	14	15	15	15
sum	6.1	6.8	7.1	6.8	7.7	7.3	9.1	10.3	10.2	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3503 Collapse assay details

Data source: Panorama

Official Gene Symbol: Timp1
Peptide Sequence: AVGNAADIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 75
Peptide End: 83

CPTAC ID: non-CPTAC-3503
Peptide Molecular Mass: 885.4668
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers