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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Kng1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Kng1 Kng
Sequence Length (AA) Molecular Weight (Da)
661 73102
Protein Name
Kininogen-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKLITTLLLC SGLLLTLTQG EEAQEIDCND EAVFQAVDFS LKQFNPGVKS
60 70 80 90 100
GNQYMLHRVI EGTKTDGSPT FYSFKYLIKE GNCSAQSGLA WQDCDFKDAE
110 120 130 140 150
EAATGECTAT VGKRENEFFI VTQTCKIAPS KAPILKAYFP CIGCVHAIST
160 170 180 190 200
DSPDLEPVLK HSIEHFNNNT DHSHLFTLRK VKSAHRQVVA GLNFDITYTI
210 220 230 240 250
VQTNCSKERF PSLHGDCVAL PNGDDGECRG NLFMDINNKI ANFSQSCTLY
260 270 280 290 300
SGDDLVEALP KPCPGCPRDI PVDSPELKEV LGHSIAQLNA ENDHPFYYKI
310 320 330 340 350
DTVKKATSQV VAGTKYVIEF IARETKCSKE SNTELAEDCE IKHLGQSLDC
360 370 380 390 400
NANVYMRPWE NKVVPTVKCQ ALDMTEMARR PPGFSPFRSV TVQETKEGRT
410 420 430 440 450
VSPPYIAREQ EERDAETEQG PTHGHGWLHE KQIKANKNHR GHKHGHDHGH
460 470 480 490 500
WSPRRHGLGH GHQKPHGLGH GHQLKLDYLR HQREDGDDHT HTVGHGHGHG
510 520 530 540 550
HGHGHGHGHG HGHGHGHGHG HGHGKHTNKD KNSVKQTTQR TESLASSSEY
560 570 580 590 600
STTSTQMQGR TEGPTLTPPR AQPTVTSSGF QDSDFIEDVV ATTPPYDTGA
610 620 630 640 650
HDDLIPDIHV QPDSLSFKLI SDFPEATSPK CPGRPWKPAS WEDPNTETTE
660 661
FSDFDLLDAL S

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-687 Collapse assay details

Data source: Panorama

Official Gene Symbol
KNG1
Peptide Modified Sequence
LNAENN[+1.0]ATFYFK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
294
Peptide - Site of Modification
6
Peptide Start
289
Peptide End
300
CPTAC ID
CPTAC-687
Peptide Molecular Mass
1,431.6670
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 6.7 7.2 8 6.4 6.6 6.5 9.3 9.8 10.3 15 15 15
y8 (1+) 8.4 6.2 5.7 6.8 7.3 5.1 10.8 9.6 7.6 15 15 15
y6 (1+) 6.8 7.4 4.4 5.8 7.8 5.2 8.9 10.8 6.8 15 15 15
sum 6.7 5.8 5.2 4.6 6 4.5 8.1 8.3 6.9 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-3506 Collapse assay details

Data source: Panorama

Official Gene Symbol
Kng1
Peptide Sequence
ATSQVVAGTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
306
Peptide End
315
CPTAC ID
non-CPTAC-3506
Peptide Molecular Mass
960.5240
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 16.7 11.7 10.8 24 7.2 15.6 29.2 13.7 19 15 15 15
y4 (1+) 15.8 12.1 8.4 16.7 11.8 11.4 23 16.9 14.2 15 15 15
y5 (1+) 8.5 8.8 10.1 13.9 11.2 11.2 16.3 14.2 15.1 15 15 15
y6 (1+) 12.9 7 12.8 16.9 9.4 8.8 21.3 11.7 15.5 15 15 15
y8 (1+) 9.3 12.6 14.4 17.5 13.1 12.2 19.8 18.2 18.9 15 15 15
sum 5.5 4.6 3.7 14.1 6.3 5.9 15.1 7.8 7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-1138 Collapse assay details

Data source: Panorama

Official Gene Symbol
KNG1
Peptide Sequence
TVGSDTFYSFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
65
Peptide End
75
CPTAC ID
non-CPTAC-1138
Peptide Molecular Mass
1,250.5819
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.2 6 2.5 8.1 7.1 3.2 9.6 9.3 4.1 15 15 15
y2 (1+) 17.2 4.9 4.7 21.8 8.9 4.7 27.8 10.2 6.6 14 15 15
y3 (1+) 7.3 2.1 5.5 12.2 6.7 5.8 14.2 7 8 15 15 15
y4 (1+) 12.7 7.6 5.6 11.7 6.6 6.8 17.3 10.1 8.8 15 15 15
y9 (1+) 8.2 6.7 7.6 9.6 9.6 8.8 12.6 11.7 11.6 15 15 15
sum 4.8 3.1 4.8 8.4 7.2 5.7 9.7 7.8 7.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments