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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Coro1a Coro1
Sequence Length (AA) Molecular Weight (Da)
461 50989
Protein Name
Coronin-1A
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSRQVVRSSK FRHVFGQPAK ADQCYEDVRV SQTTWDSGFC AVNPKFMALI
60 70 80 90 100
CEASGGGAFL VLPLGKTGRV DKNVPLVCGH TAPVLDIAWC PHNDNVIASG
110 120 130 140 150
SEDCTVMVWE IPDGGLVLPL REPVITLEGH TKRVGIVAWH PTAQNVLLSA
160 170 180 190 200
GCDNVILVWD VGTGAAVLTL GPDVHPDTIY SVDWSRDGAL ICTSCRDKRV
210 220 230 240 250
RVIEPRKGTV VAEKDRPHEG TRPVHAVFVS EGKILTTGFS RMSERQVALW
260 270 280 290 300
DTKHLEEPLS LQELDTSSGV LLPFFDPDTN IVYLCGKGDS SIRYFEITSE
310 320 330 340 350
APFLHYLSMF SSKESQRGMG YMPKRGLEVN KCEIARFYKL HERKCEPIAM
360 370 380 390 400
TVPRKSDLFQ EDLYPPTAGP DPALTAEEWL GGRDAGPLLI SLKDGYVPPK
410 420 430 440 450
SRELRVNRGL DSARRRATPE PSGTPSSDTV SRLEEDVRNL NAIVQKLQER
460 461
LDRLEETVQA K

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3507 Collapse assay details

Data source: Panorama

Official Gene Symbol
Coro1a
Peptide Sequence
DAGPLLISLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
384
Peptide End
393
CPTAC ID
non-CPTAC-3507
Peptide Molecular Mass
1,025.6121
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 15.7 7.6 15.2 21.4 11.6 16.4 26.5 13.9 22.4 15 15 15
y8 (2+) 16.6 17.8 11.9 20.8 21 15.8 26.6 27.5 19.8 15 15 15
y4 (1+) 14.3 24 37.6 44.4 42.1 56.5 46.6 48.5 67.9 15 15 15
y5 (1+) 27 18.7 23 38.6 28.9 29.1 47.1 34.4 37.1 15 15 15
y8 (1+) 9.2 13.8 8.9 16.8 12.6 14.5 19.2 18.7 17 15 15 15
sum 6.6 9.7 6.7 17.3 12.8 12.2 18.5 16.1 13.9 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3905 Collapse assay details

Data source: Panorama

Official Gene Symbol
Coro1a
Peptide Sequence
HVFGQPAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
13
Peptide End
20
CPTAC ID
non-CPTAC-3905
Peptide Molecular Mass
882.4712
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 18.6 9.2 10.2 22.7 8.2 10.8 29.3 12.3 14.9 15 15 15
b2 (1+) 9.3 6.6 3.6 14.3 6 6.9 17.1 8.9 7.8 15 15 15
y3 (1+) 31.3 6.4 4.7 30.1 7.8 7.2 43.4 10.1 8.6 15 15 15
y6 (1+) 25.5 9.9 8 26.7 9.6 9.8 36.9 13.8 12.7 15 15 15
y7 (1+) 20 20.9 11 24.8 39.2 24.6 31.9 44.4 26.9 15 15 15
sum 4.6 3.9 4.3 4.6 10.2 6.6 6.5 10.9 7.9 15 15 15


Additional Resources and Comments