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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ambp Itil
Sequence Length (AA) Molecular Weight (Da)
349 39029
Protein Name
Protein AMBP
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQGLRTLFLL LTACLASRAD PASTLPDIQV QENFSESRIY GKWYNLAVGS
60 70 80 90 100
TCPWLSRIKD KMSVSTLVLQ EGATETEISM TSTRWRRGVC EEITGAYQKT
110 120 130 140 150
DIDGKFLYHK SKWNITLESY VVHTNYDEYA IFLTKKSSHH HGLTITAKLY
160 170 180 190 200
GREPQLRDSL LQEFKDVALN VGISENSIIF MPDRGECVPG DREVEPTSIA
210 220 230 240 250
RARRAVLPQE SEGSGTEPLI TGTLKKEDSC QLNYSEGPCL GMQERYYYNG
260 270 280 290 300
ASMACETFQY GGCLGNGNNF ISEKDCLQTC RTIAACNLPI VQGPCRAFIK
310 320 330 340 349
LWAFDAAQGK CIQFHYGGCK GNGNKFYSEK ECKEYCGVPG DGYEELIRS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5576 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ambp
Peptide Sequence
DSLLQEFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
158
Peptide End
165
CPTAC ID
non-CPTAC-5576
Peptide Molecular Mass
978.5022
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.3 5.4 6.6 8.9 9.5 6.5 10.4 10.9 9.3 15 15 15
y6 (2+) 6.8 8.4 4.1 14.2 9.9 6.6 15.7 13 7.8 15 15 15
y4 (1+) 7.8 5.3 3.4 11.3 6.5 4.7 13.7 8.4 5.8 15 15 15
y5 (1+) 8 5.3 4.7 8.7 8.3 4.3 11.8 9.8 6.4 15 15 15
y6 (1+) 7.6 4.1 2.8 9.7 6.3 4.8 12.3 7.5 5.6 15 15 15
sum 2.8 2.3 2 5.1 6.5 3 5.8 6.9 3.6 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1153 Collapse assay details

Data source: Panorama

Official Gene Symbol
AMBP
Peptide Sequence
ETLLQDFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
159
Peptide End
166
CPTAC ID
non-CPTAC-1153
Peptide Molecular Mass
1,020.5240
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 12.2 7.3 4.3 12.8 6.2 4 17.7 9.6 5.9 15 15 15
y6 (2+) 8.8 5.7 7.1 12.1 3.5 6.5 15 6.7 9.6 15 15 15
y3 (1+) 7.1 5.1 5.9 11.1 7 5.9 13.2 8.7 8.3 15 15 15
y4 (1+) 4 5.2 5.1 8.6 6.3 6.1 9.5 8.2 8 15 15 15
y5 (1+) 7.5 4.6 3.7 6 5.4 3.8 9.6 7.1 5.3 15 15 15
sum 3.8 3.8 2.5 7.1 4.7 3.4 8.1 6 4.2 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3510 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ambp
Peptide Sequence
EVEPTSIAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
193
Peptide End
201
CPTAC ID
non-CPTAC-3510
Peptide Molecular Mass
1,000.5189
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 8.4 10.2 5.5 14.1 9.7 10 16.4 14.1 11.4 15 15 15
y7 (2+) 37.6 28.8 37 30.9 23.4 39.7 48.7 37.1 54.3 15 15 15
y2 (1+) 15.8 14.6 15.9 20.7 18.2 16.5 26 23.3 22.9 15 15 15
y6 (1+) 13.9 10.9 9.3 17.1 11.3 8.1 22 15.7 12.3 15 15 15
y7 (1+) 18.7 27.4 16 18.4 26.3 23.7 26.2 38 28.6 15 15 15
sum 8.4 7.5 5.1 11.8 6.9 8 14.5 10.2 9.5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1152 Collapse assay details

Data source: Panorama

Official Gene Symbol
AMBP
Peptide Sequence
HHGPTITAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
141
Peptide End
149
CPTAC ID
non-CPTAC-1152
Peptide Molecular Mass
960.5141
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 8.2 5.3 5.8 11.8 10.3 14.6 14.4 11.6 15.7 15 15 15
b2 (1+) 7.5 7.3 6.9 12 10.4 11.2 14.2 12.7 13.2 15 15 15
y6 (3+) NA NA NA NA NA NA NA NA NA 0 0 0
y2 (1+) 10.2 2.7 3.6 13.4 4.6 5.6 16.8 5.3 6.7 15 15 15
y3 (1+) 6.4 4.8 6.1 8.7 6.1 7.6 10.8 7.8 9.7 15 15 15
sum 3.6 3.7 2.9 6.3 4.9 3.6 7.3 6.1 4.6 15 15 15

Additional Resources and Comments