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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Vasn

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Vasn Slitl2
Sequence Length (AA) Molecular Weight (Da)
673 72261
Protein Name
Vasorin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MHSRSCLPPL LLLLLVLLGS GVQGCPSGCQ CNQPQTVFCT ARQGTTVPRD
60 70 80 90 100
VPPDTVGLYI FENGITTLDV GCFAGLPGLQ LLDLSQNQIT SLPGGIFQPL
110 120 130 140 150
VNLSNLDLTA NKLHEISNET FRGLRRLERL YLGKNRIRHI QPGAFDALDR
160 170 180 190 200
LLELKLPDNE LRVLPPLHLP RLLLLDLSHN SIPALEAGIL DTANVEALRL
210 220 230 240 250
AGLGLRQLDE GLFGRLLNLH DLDVSDNQLE HMPSVIQGLR GLTRLRLAGN
260 270 280 290 300
TRIAQIRPED LAGLTALQEL DVSNLSLQAL PSDLSSLFPR LRLLAAARNP
310 320 330 340 350
FNCLCPLSWF GPWVRENHVV LASPEETRCH FPPKNAGRLL LDLDYADFGC
360 370 380 390 400
PVTTTTATVP TIRSTIREPT LSTSSQAPTW PSLTEPTTQA STVLSTAPPT
410 420 430 440 450
MRPAPQPQDC PASICLNGGS CRLGARHHWE CLCPEGFIGL YCESPVEQGM
460 470 480 490 500
KPSSIPDTPR PPPLLPLSIE PVSPTSLRVK LQRYLQGNTV QLRSLRLTYR
510 520 530 540 550
NLSGPDKRLV TLRLPASLAE YTVTQLRPNA TYSICVTPLG AGRTPEGEEA
560 570 580 590 600
CGEANTSQAV RSNHAPVTQA REGNLPLLIA PALAAVLLAV LAAAGAAYCV
610 620 630 640 650
RRARATSTAQ DKGQVGPGTG PLELEGVKAP LEPGSKATEG GGEALSGGPE
660 670 673
CEVPLMGYPG PSLQGVLPAK HYI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3511 Collapse assay details

Data source: Panorama

Official Gene Symbol
Vasn
Peptide Sequence
ENHVVLASPEETR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
316
Peptide End
328
CPTAC ID
non-CPTAC-3511
Peptide Molecular Mass
1,479.7318
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b8 (2+) 10.5 10.5 9.1 19.3 15.2 12.5 22 18.5 15.5 15 15 15
y5 (2+) 5.7 4.2 8.1 13.5 6.8 7.9 14.7 8 11.3 15 15 15
y6 (2+) 13.9 9 10.5 16.7 11.5 25.5 21.7 14.6 27.6 15 15 15
y4 (1+) 4.7 14.4 25.2 20.7 10.5 29 21.2 17.8 38.4 15 15 15
y5 (1+) 7.9 5 8.1 15.8 5.8 8.8 17.7 7.7 12 15 15 15
sum 5.7 3.2 6.6 14 4.5 7.6 15.1 5.5 10.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-1166 Collapse assay details

Data source: Panorama

Official Gene Symbol
VASN
Peptide Sequence
ESHVTLASPEETR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
315
Peptide End
327
CPTAC ID
non-CPTAC-1166
Peptide Molecular Mass
1,454.7001
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 14.4 8.2 8.4 18.9 11.1 9.7 23.8 13.8 12.8 12 15 15
y5 (2+) 15.3 4.3 7.5 19.8 5.5 7.6 25 7 10.7 14 15 15
y6 (2+) 36.5 17.8 6.7 37.6 16.5 7.7 52.4 24.3 10.2 12 15 15
y2 (1+) 16.9 8.7 6.6 16.9 11 7.7 23.9 14 10.1 10 15 15
y5 (1+) 11.5 8.7 6.7 15 8.6 7.1 18.9 12.2 9.8 12 15 15
sum 22.2 5.2 5.7 25.2 6 5.6 33.6 7.9 8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments