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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Vtn N/A
Sequence Length (AA) Molecular Weight (Da)
478 54849
Protein Name
Vitronectin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPLRPFFIL ALVAWVSLAD QESCKGRCTQ GFMASKKCQC DELCTYYQSC
60 70 80 90 100
CADYMEQCKP QVTRGDVFTM PEDDYWSYDY VEEPKNNTNT GVQPENTSPP
110 120 130 140 150
GDLNPRTDGT LKPTAFLDPE EQPSTPAPKV EQQEEILRPD TTDQGTPEFP
160 170 180 190 200
EEELCSGKPF DAFTDLKNGS LFAFRGQYCY ELDETAVRPG YPKLIQDVWG
210 220 230 240 250
IEGPIDAAFT RINCQGKTYL FKGSQYWRFE DGVLDPGYPR NISEGFSGIP
260 270 280 290 300
DNVDAAFALP AHRYSGRERV YFFKGKQYWE YEFQQQPSQE ECEGSSLSAV
310 320 330 340 350
FEHFALLQRD SWENIFELLF WGRSSDGARE PQFISRNWHG VPGKVDAAMA
360 370 380 390 400
GRIYVTGSLS HSAQAKKQKS KRRSRKRYRS RRGRGHRRSQ SSNSRRSSRS
410 420 430 440 450
IWFSLFSSEE SGLGTYNNYD YDMDWLVPAT CEPIQSVYFF SGDKYYRVNL
460 470 478
RTRRVDSVNP PYPRSIAQYW LGCPTSEK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-690 Collapse assay details

Data source: Panorama

Official Gene Symbol
VTN
Peptide Modified Sequence
N[+1.0]GSLFAFR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
169
Peptide - Site of Modification
1
Peptide Start
169
Peptide End
176
CPTAC ID
CPTAC-690
Peptide Molecular Mass
911.4501
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 8.7 7.7 9 8.3 8.1 9.5 12 11.2 13.1 15 15 15
y5 (1+) 4 6.1 7.1 4.8 5.7 7.2 6.2 8.3 10.1 15 15 15
y4 (1+) 4.9 5.3 6.2 4.8 5.6 6.3 6.9 7.7 8.8 15 15 15
sum 4.2 5.2 6.2 4.4 5.4 6.2 6.1 7.5 8.8 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3512 Collapse assay details

Data source: Panorama

Official Gene Symbol
Vtn
Peptide Sequence
EPQFISR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
330
Peptide End
336
CPTAC ID
non-CPTAC-3512
Peptide Molecular Mass
875.4501
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (2+) 15.1 4.2 2.8 19.7 4.6 6.5 24.8 6.2 7.1 15 15 15
y2 (1+) 5.4 2.3 3.8 15.9 3.8 6.1 16.8 4.4 7.2 15 15 15
y3 (1+) 7.3 4.1 2.9 14.9 6.1 5.2 16.6 7.3 6 15 15 15
y4 (1+) 7.7 3.4 3.9 17 3.6 7.5 18.7 5 8.5 15 15 15
y5 (1+) 10.3 4 4 13.2 8.9 7 16.7 9.8 8.1 15 15 15
sum 4.4 1.3 1.9 13.9 3.5 5.3 14.6 3.7 5.6 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3513 Collapse assay details

Data source: Panorama

Official Gene Symbol
Vtn
Peptide Sequence
IYVTGSLSHSAQAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
353
Peptide End
366
CPTAC ID
non-CPTAC-3513
Peptide Molecular Mass
1,460.7623
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6 6 5.1 14.8 6.8 6.9 16 9.1 8.6 15 15 15
y10 (2+) 11.4 5.4 5.8 18.5 8.5 6.8 21.7 10.1 8.9 15 15 15
y11 (2+) 8.1 3.9 4.7 14.5 6 6.8 16.6 7.2 8.3 15 15 15
y12 (2+) 6.7 3.8 4.8 16.5 9.4 9.1 17.8 10.1 10.3 15 15 15
y13 (2+) 4.7 4.9 6 14.2 5.3 8.9 15 7.2 10.7 15 15 15
sum 2.8 1.9 2.8 13.9 5.2 6.2 14.2 5.5 6.8 15 15 15

Additional Resources and Comments