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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Gsn Gsb
Sequence Length (AA) Molecular Weight (Da)
780 85942
Protein Name
Gelsolin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPYRSSLLC ALLLLALCAL SPSHAATTSR GRAQERAPQS RVSEARPSTM
60 70 80 90 100
VVEHPEFLKA GKEPGLQIWR VEKFDLVPVP PNLYGDFFTG DAYVILKTVQ
110 120 130 140 150
LRNGNLQYDL HYWLGNECSQ DESGAAAIFT VQLDDYLNGR AVQHREVQGF
160 170 180 190 200
ESSTFSGYFK SGLKYKKGGV ASGFKHVVPN EVVVQRLFQV KGRRVVRATE
210 220 230 240 250
VPVSWDSFNN GDCFILDLGN NIYQWCGSGS NKFERLKATQ VSKGIRDNER
260 270 280 290 300
SGRAQVHVSE EGGEPEAMLQ VLGPKPALPE GTEDTAKEDA ANRRLAKLYK
310 320 330 340 350
VSNGAGSMSV SLVADENPFA QGALRSEDCF ILDHGRDGKI FVWKGKQANM
360 370 380 390 400
EERKAALKTA SDFISKMQYP RQTQVSVLPE GGETPLFKQF FKNWRDPDQT
410 420 430 440 450
DGPGLGYLSS HIANVERVPF DAATLHTSTA MAAQHGMDDD GTGQKQIWRI
460 470 480 490 500
EGSNKVPVDP ATYGQFYGGD SYIILYNYRH GGRQGQIIYN WQGAQSTQDE
510 520 530 540 550
VAASAILTAQ LDEELGGTPV QSRVVQGKEP AHLMSLFGGK PMIIYKGGTS
560 570 580 590 600
RDGGQTAPAS IRLFQVRASS SGATRAVEVM PKSGALNSND AFVLKTPSAA
610 620 630 640 650
YLWVGAGASE AEKTGAQELL KVLRSQHVQV EEGSEPDAFW EALGGKTAYR
660 670 680 690 700
TSPRLKDKKM DAHPPRLFAC SNRIGRFVIE EVPGELMQED LATDDVMLLD
710 720 730 740 750
TWDQVFVWVG KDSQEEEKTE ALTSAKRYIE TDPANRDRRT PITVVRQGFE
760 770 780
PPSFVGWFLG WDDNYWSVDP LDRALAELAA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1127 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSN
Peptide Sequence
AGALNSNDAFVLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
585
Peptide End
597
CPTAC ID
non-CPTAC-1127
Peptide Molecular Mass
1,318.6881
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 4.1 2.7 5 5.1 5.4 4.9 6.5 6 7 15 15 15
b3 (1+) 6 4.3 3.6 7.5 4.3 4 9.6 6.1 5.4 15 15 15
y2 (1+) 5.8 8.7 6.4 11.2 8.9 6.1 12.6 12.4 8.8 15 15 15
y8 (1+) 5 6.5 5.4 11.7 6.9 5.4 12.7 9.5 7.6 15 15 15
y9 (1+) 4.7 4.5 3.9 9.7 5.4 4.8 10.8 7 6.2 15 15 15
sum 2.8 3 2.8 7.1 3.7 2.5 7.6 4.8 3.8 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3366 Collapse assay details

Data source: Panorama

Official Gene Symbol
Gsn
Peptide Sequence
GGVASGFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
168
Peptide End
175
CPTAC ID
non-CPTAC-3366
Peptide Molecular Mass
721.3759
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 7.1 4.5 4.8 15.9 7 7.7 17.4 8.3 9.1 15 15 15
y2 (1+) 12.3 9.5 7.4 12.4 9.9 7.8 17.5 13.7 10.8 15 15 15
y4 (1+) 5.6 6.4 4.8 12.1 8.7 7 13.3 10.8 8.5 15 15 15
y5 (1+) 6.2 6.8 5.2 12.9 3.5 7.2 14.3 7.6 8.9 15 15 15
y6 (1+) 7.3 7.8 6.9 16.6 6.5 6.7 18.1 10.2 9.6 15 15 15
sum 4.5 3.5 3.9 12.7 2.9 5.2 13.5 4.5 6.5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3514 Collapse assay details

Data source: Panorama

Official Gene Symbol
Gsn
Peptide Sequence
HVVPNEVVVQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
176
Peptide End
186
CPTAC ID
non-CPTAC-3514
Peptide Molecular Mass
1,274.7095
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.8 5.9 3.9 14.4 7.8 8.2 15.5 9.8 9.1 15 15 15
b6 (1+) 4.1 5.3 4.7 14.3 5.9 10.5 14.9 7.9 11.5 15 15 15
b2 (1+) 7 6.7 6.7 16 6.5 8.8 17.5 9.3 11.1 15 15 15
y3 (1+) 6.2 6.7 8.3 28.2 6.9 8.5 28.9 9.6 11.9 15 15 15
y4 (1+) 3.8 4.9 2.5 11.1 5.6 7 11.7 7.4 7.4 15 15 15
sum 2.6 2.4 1.9 12.1 4 5.9 12.4 4.7 6.2 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1126 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSN
Peptide Sequence
TGAQELLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
616
Peptide End
623
CPTAC ID
non-CPTAC-1126
Peptide Molecular Mass
886.4872
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 13.6 7.7 5.9 17.1 8.6 6.5 21.8 11.5 8.8 15 15 15
y3 (1+) 15.2 11.5 9 21.5 11.4 9.3 26.3 16.2 12.9 15 15 15
y4 (1+) 14.9 9 6.8 15.1 10.2 9.8 21.2 13.6 11.9 15 15 15
y5 (1+) 8.2 10.6 6.1 11.3 11.2 6 14 15.4 8.6 15 15 15
y7 (1+) 17.4 9.6 8.8 16.1 11.6 6.9 23.7 15.1 11.2 14 15 15
sum 7.7 5.4 4.5 8.3 6.5 5 11.3 8.5 6.7 15 15 15

Additional Resources and Comments