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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Lifr N/A
Sequence Length (AA) Molecular Weight (Da)
1092 122574
Protein Name
Leukemia inhibitory factor receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAYSWWRQP SWMVDNKRSR MTPNLPWLLS ALTLLHLTMH ANGLKRGVQD
60 70 80 90 100
LKCTTNNMRV WDCTWPAPLG VSPGTVKDIC IKDRFHSCHP LETTNVKIPA
110 120 130 140 150
LSPGDHEVTI NYLNGFQSKF TLNEKDVSLI PETPEILDLS ADFFTSSLLL
160 170 180 190 200
KWNDRGSALP HPSNATWEIK VLQNPRTEPV ALVLLNTMLS GKDTVQHWNW
210 220 230 240 250
TSDLPLQCAT HSVSIRWHID SPHFSGYKEW SDWSPLKNIS WIRNTETNVF
260 270 280 290 300
PQDKVVLAGS NMTICCMSPT KVLSGQIGNT LRPLIHLYGQ TVAIHILNIP
310 320 330 340 350
VSENSGTNII FITDDDVYGT VVFAGYPPDV PQKLSCETHD LKEIICSWNP
360 370 380 390 400
GRITGLVGPR NTEYTLFESI SGKSAVFHRI EGLTNETYRL GVQMHPGQEI
410 420 430 440 450
HNFTLTGRNP LGQAQSAVVI NVTERVAPHD PTSLKVKDIN STVVTFSWYL
460 470 480 490 500
PGNFTKINLL CQIEICKANS KKEVRNATIR GAEDSTYHVA VDKLNPYTAY
510 520 530 540 550
TFRVRCSSKT FWKWSRWSDE KRHLTTEATP SKGPDTWREW SSDGKNLIVY
560 570 580 590 600
WKPLPINEAN GKILSYNVSC SLNEETQSVL EIFDPQHRAE IQLSKNDYII
610 620 630 640 650
SVVARNSAGS SPPSKIASME IPNDDITVEQ AVGLGNRIFL TWRHDPNMTC
660 670 680 690 700
DYVIKWCNSS RSEPCLLDWR KVPSNSTETV IESDQFQPGV RYNFYLYGCT
710 720 730 740 750
NQGYQLLRSI IGYVEELAPI VAPNFTVEDT SADSILVKWD DIPVEELRGF
760 770 780 790 800
LRGYLFYFQK GERDTPKTRS LEPHHSDIKL KNITDISQKT LRIADLQGKT
810 820 830 840 850
SYHLVLRAYT HGGLGPEKSM FVVTKENSVG LIIAILIPVA VAVIVGVVTS
860 870 880 890 900
ILCYRKREWI KETFYPDIPN PENCKALQFQ KSVCEGSNAL KTLEMNPCTP
910 920 930 940 950
NNVEVLESRS IVPKIEDTEI ISPVAERPGE RSEVDPENHV VVSYCPPIIE
960 970 980 990 1000
EEITNPAADE VGGASQVVYI DVQSMYQPQA KAEEEQDVDP VVVAGYKPQM
1010 1020 1030 1040 1050
RLPISPAVED TAAEDEEGKT AGYRPQANVN TWNLVSPDSP RSTDSNNEVV
1060 1070 1080 1090 1092
SFGSPCSINS RQFLIPPKDE DSPKSNGGGW SFTNFFQNKP ND

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3515 Collapse assay details

Data source: Panorama

Official Gene Symbol
Lifr
Peptide Sequence
NTETNVFPQDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
244
Peptide End
254
CPTAC ID
non-CPTAC-3515
Peptide Molecular Mass
1,291.6044
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5 7.9 5 14 8.2 7.7 14.9 11.4 9.2 15 15 15
y9 (1+) 8.7 6 6.2 14.3 7.5 9 16.7 9.6 10.9 15 15 15
b2 (1+) 7.3 4.3 4.2 18.1 6.9 7.3 19.5 8.1 8.4 15 15 15
y4 (1+) 4.5 5.4 4.1 16 6.9 7.2 16.6 8.8 8.3 15 15 15
y5 (1+) 15.8 7.2 5.7 16 8.6 7.8 22.5 11.2 9.7 15 15 15
sum 5.9 1.6 2.7 12.5 4.1 5.9 13.8 4.4 6.5 15 15 15


Additional Resources and Comments