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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ighg1 Igh-4
Sequence Length (AA) Molecular Weight (Da)
393 43387
Protein Name
Ig gamma-1 chain C region, membrane-bound form
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
AKTTPPSVYP LAPGSAAQTN SMVTLGCLVK GYFPEPVTVT WNSGSLSSGV
60 70 80 90 100
HTFPAVLQSD LYTLSSSVTV PSSPRPSETV TCNVAHPASS TKVDKKIVPR
110 120 130 140 150
DCGCKPCICT VPEVSSVFIF PPKPKDVLTI TLTPKVTCVV VDISKDDPEV
160 170 180 190 200
QFSWFVDDVE VHTAQTQPRE EQFNSTFRSV SELPIMHQDW LNGKEFKCRV
210 220 230 240 250
NSAAFPAPIE KTISKTKGRP KAPQVYTIPP PKEQMAKDKV SLTCMITDFF
260 270 280 290 300
PEDITVEWQW NGQPAENYKN TQPIMNTNGS YFVYSKLNVQ KSNWEAGNTF
310 320 330 340 350
TCSVLHEGLH NHHTEKSLSH SPGLQLDETC AEAQDGELDG LWTTITIFIS
360 370 380 390 393
LFLLSVCYSA AVTLFKVKWI FSSVVELKQT LVPEYKNMIG QAP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-669 Collapse assay details

Data source: Panorama

Official Gene Symbol
IGHG1
Peptide Modified Sequence
EEQYN[+1.0]STYR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
180
Peptide - Site of Modification
5
Peptide Start
176
Peptide End
184
CPTAC ID
CPTAC-669
Peptide Molecular Mass
1,189.4888
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 3.1 3.2 1.9 3.9 4.2 2.6 5 5.3 3.2 15 15 15
y6 (1+) 2 2.7 2.1 3.2 3.5 3.2 3.8 4.4 3.8 15 15 15
y5 (1+) 3.5 2.4 2.6 3.2 3.1 3.5 4.7 3.9 4.4 15 15 15
sum 2.6 2.3 2 2.7 3.2 3 3.7 3.9 3.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3532 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ighg1
Peptide Sequence
DVLTITLTPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
126
Peptide End
135
CPTAC ID
non-CPTAC-3532
Peptide Molecular Mass
1,099.6489
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5.3 4.3 5.4 17.4 7 5.8 18.2 8.2 7.9 15 15 15
b4 (2+) 5.2 5.9 3.6 12.3 7 6.3 13.4 9.2 7.3 15 15 15
y2 (1+) 4.8 5.2 5.8 12.8 6.4 6.7 13.7 8.2 8.9 15 15 15
y3 (1+) 2.8 3.8 6.6 11 4.4 6.9 11.4 5.8 9.5 15 15 15
y7 (1+) 5.8 4.5 3.5 11.1 6.5 8.3 12.5 7.9 9 15 15 15
sum 1.6 1.6 2.5 12.5 4 5.2 12.6 4.3 5.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3533 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ighg1
Peptide Sequence
VNSAAFPAPIEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
200
Peptide End
211
CPTAC ID
non-CPTAC-3533
Peptide Molecular Mass
1,242.6608
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7.1 6.4 5.5 13.3 6.6 6.8 15.1 9.2 8.7 15 15 15
b2 (1+) 6.7 5.2 8.8 13.9 6.2 9.4 15.4 8.1 12.9 15 15 15
y4 (1+) 3.8 3.6 6 12.2 5.4 9.6 12.8 6.5 11.3 15 15 15
y6 (1+) 6.7 5.4 6.8 15.9 9.5 6.6 17.3 10.9 9.5 15 15 15
y7 (1+) 4.5 8.4 4.9 14.1 8.5 9 14.8 12 10.2 15 15 15
sum 2.9 1.8 2.9 13.5 5.3 6 13.8 5.6 6.7 15 15 15


Additional Resources and Comments