Itih1, CPTAC-3534 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Itih1	N/A

Sequence Length (AA)	Molecular Weight (Da)
907	101067

Protein Name
Inter-alpha-trypsin inhibitor heavy chain H1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEGATGLRVL	LCLLPLLTLQ	ARPALGLATG	RPRGSEKRHA	VDTSVNGVSI
60	70	80	90	100
KSLKVNCKVT	SRFAHYVITS	QVVNNADKAR	EVAFDVEIPK	TAFISDFAIT
110	120	130	140	150
SDGKAFIGDI	KDKVTAWKQY	RKAAVLGESA	GLVRASGRNM	EQFTIHITVG
160	170	180	190	200
AQSKATFRLT	YEEVLKRRLM	QYDITIKVRP	KQLVQHFEID	VDIFEPQGIS
210	220	230	240	250
KLDAQASFLS	EELAAQTIKK	SFSGKKGHVL	FRPTVSQQQS	CPTCSTSLLN
260	270	280	290	300
GEFKVTYDVN	RDKLCDLLVA	NNYFTHFFAP	KNLTNMSKNL	VFVIDISGSM
310	320	330	340	350
EGQKVRQTKE	ALLKILEDMR	PVDNFDLVLF	GSKVQSWKGS	LVPASNANLQ
360	370	380	390	400
AAQDFVRRFS	LAGATNLNGG	LLRGIEILNK	AQGSHPELSS	PASILIMLTD
410	420	430	440	450
GEPTEGETDR	SQILKNVRNA	IRGRFPLYNL	GFGHDLDFSF	LEVMSTENNG
460	470	480	490	500
WAQRIYEDHD	ATQQLQGFYN	QVANPLLTDV	ELQYPQDAVL	ALTQHRHKQY
510	520	530	540	550
YDGSEIVVAG	RIANHKLNTF	KADVRARGEK	QEFRATCLVD	EEEMKKLLRE
560	570	580	590	600
RGHVLENHVE	RLWAYLTIQE	LLAKRMKTEG	EERANLSSQV	LKMSLDYHFV
610	620	630	640	650
TPLTSLTIRG	LTDEDGLEPT	IDKPLEDSQP	LEMVGPRRTF	VLSAIQPSPT
660	670	680	690	700
AHPIDSKLPL	RVTGVDTDPH	FIIYVPSKED	SLCFNINEEP	GVILNLVQDP
710	720	730	740	750
DTGFTVNGQL	IGNKASSPGQ	HESTYFGRLG	ISSPTSDFQL	EVTPQNITLN
760	770	780	790	800
PSSSGSMFSW	RDQAVLQKDG	VVVTINKKRN	LVVSVDDGAT	FEIVLHRTWK
810	820	830	840	850
GSAVHQDFLG	FYVLDSFRMS	ARTKGLLGQF	FSPLDFEVFD	LHPGSDPTKT
860	870	880	890	900
DATMVVKNRQ	LTVTRGLQKD	YSKDPRHGAE	VPCWFVHDNG	AGLIDGVHTD
907
YVVSDIF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3832 Collapse assay details

Data source: Panorama

Official Gene Symbol: Itih1
Peptide Sequence: AAVLGESAGLVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 123
Peptide End: 134

CPTAC ID: non-CPTAC-3832
Peptide Molecular Mass: 1,141.6455
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	6	6.6	3.9	11.4	8.2	5.2	12.9	10.5	6.5	15	15	15
b4 (1+)	11.4	6.5	6.4	15.5	7	5.6	19.2	9.6	8.5	15	15	15
y9 (1+)	8.7	3.9	6	12	5.3	7.3	14.8	6.6	9.4	15	15	15
y8 (1+)	6.1	6.8	6	7	7.5	6.6	9.3	10.1	8.9	15	15	15
y10 (2+)	16.1	7.7	3	15	8.6	4.1	22	11.5	5.1	15	15	15
sum	4.9	3.7	2.2	10.8	4.4	3.3	11.9	5.7	4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3534 Collapse assay details

Data source: Panorama

Official Gene Symbol: Itih1
Peptide Sequence: FAHYVITSQVVNNADK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 63
Peptide End: 78

CPTAC ID: non-CPTAC-3534
Peptide Molecular Mass: 1,804.9108
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.