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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Itih1 N/A
Sequence Length (AA) Molecular Weight (Da)
907 101067
Protein Name
Inter-alpha-trypsin inhibitor heavy chain H1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEGATGLRVL LCLLPLLTLQ ARPALGLATG RPRGSEKRHA VDTSVNGVSI
60 70 80 90 100
KSLKVNCKVT SRFAHYVITS QVVNNADKAR EVAFDVEIPK TAFISDFAIT
110 120 130 140 150
SDGKAFIGDI KDKVTAWKQY RKAAVLGESA GLVRASGRNM EQFTIHITVG
160 170 180 190 200
AQSKATFRLT YEEVLKRRLM QYDITIKVRP KQLVQHFEID VDIFEPQGIS
210 220 230 240 250
KLDAQASFLS EELAAQTIKK SFSGKKGHVL FRPTVSQQQS CPTCSTSLLN
260 270 280 290 300
GEFKVTYDVN RDKLCDLLVA NNYFTHFFAP KNLTNMSKNL VFVIDISGSM
310 320 330 340 350
EGQKVRQTKE ALLKILEDMR PVDNFDLVLF GSKVQSWKGS LVPASNANLQ
360 370 380 390 400
AAQDFVRRFS LAGATNLNGG LLRGIEILNK AQGSHPELSS PASILIMLTD
410 420 430 440 450
GEPTEGETDR SQILKNVRNA IRGRFPLYNL GFGHDLDFSF LEVMSTENNG
460 470 480 490 500
WAQRIYEDHD ATQQLQGFYN QVANPLLTDV ELQYPQDAVL ALTQHRHKQY
510 520 530 540 550
YDGSEIVVAG RIANHKLNTF KADVRARGEK QEFRATCLVD EEEMKKLLRE
560 570 580 590 600
RGHVLENHVE RLWAYLTIQE LLAKRMKTEG EERANLSSQV LKMSLDYHFV
610 620 630 640 650
TPLTSLTIRG LTDEDGLEPT IDKPLEDSQP LEMVGPRRTF VLSAIQPSPT
660 670 680 690 700
AHPIDSKLPL RVTGVDTDPH FIIYVPSKED SLCFNINEEP GVILNLVQDP
710 720 730 740 750
DTGFTVNGQL IGNKASSPGQ HESTYFGRLG ISSPTSDFQL EVTPQNITLN
760 770 780 790 800
PSSSGSMFSW RDQAVLQKDG VVVTINKKRN LVVSVDDGAT FEIVLHRTWK
810 820 830 840 850
GSAVHQDFLG FYVLDSFRMS ARTKGLLGQF FSPLDFEVFD LHPGSDPTKT
860 870 880 890 900
DATMVVKNRQ LTVTRGLQKD YSKDPRHGAE VPCWFVHDNG AGLIDGVHTD
907
YVVSDIF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3832 Collapse assay details

Data source: Panorama

Official Gene Symbol
Itih1
Peptide Sequence
AAVLGESAGLVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
123
Peptide End
134
CPTAC ID
non-CPTAC-3832
Peptide Molecular Mass
1,141.6455
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 6 6.6 3.9 11.4 8.2 5.2 12.9 10.5 6.5 15 15 15
b4 (1+) 11.4 6.5 6.4 15.5 7 5.6 19.2 9.6 8.5 15 15 15
y9 (1+) 8.7 3.9 6 12 5.3 7.3 14.8 6.6 9.4 15 15 15
y8 (1+) 6.1 6.8 6 7 7.5 6.6 9.3 10.1 8.9 15 15 15
y10 (2+) 16.1 7.7 3 15 8.6 4.1 22 11.5 5.1 15 15 15
sum 4.9 3.7 2.2 10.8 4.4 3.3 11.9 5.7 4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3534 Collapse assay details

Data source: Panorama

Official Gene Symbol
Itih1
Peptide Sequence
FAHYVITSQVVNNADK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
63
Peptide End
78
CPTAC ID
non-CPTAC-3534
Peptide Molecular Mass
1,804.9108
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.6 7.9 11.3 16.2 10.9 10.6 17.5 13.5 15.5 15 15 15
y7 (1+) 10 8.1 5.2 14.7 10.7 7.8 17.8 13.4 9.4 15 15 15
b10 (2+) 7.3 8.9 9.3 14.6 7.5 8.6 16.3 11.6 12.7 15 15 15
b9 (2+) 8.2 10.3 10.2 22.1 10.5 7.8 23.6 14.7 12.8 15 15 15
b3 (1+) 8.1 6.4 4.6 17 5.7 10 18.8 8.6 11 15 15 15
sum 3.8 4.7 4.7 13.9 5.2 5.6 14.4 7 7.3 15 15 15


Additional Resources and Comments