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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Bmp1 N/A
Sequence Length (AA) Molecular Weight (Da)
991 111666
Protein Name
Bone morphogenetic protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPGVARPPLP LLSLPLLLLL LLLPRAGRPL DLADYTYDLG EEDAPELLNY
60 70 80 90 100
KDPCKAAAFL GDIALDEEDL RAFQVQQAAV LRQQTARRPS IKAAGNSSAL
110 120 130 140 150
GGQGTSGQPQ RESRGRWRGR PRSRRAATSR PERVWPDGVI PFVIGGNFTG
160 170 180 190 200
SQRAVFRQAM RHWEKHTCVT FLERTDEDSY IVFTYRPCGC CSYVGRRGGG
210 220 230 240 250
PQAISIGKNC DKFGIVVHEL GHVIGFWHEH TRPDRDRHVS IVRENIQPGQ
260 270 280 290 300
EYNFLKMEVQ EVESLGETYD FDSIMHYARN TFSRGIFLDT IVPKYEVNGV
310 320 330 340 350
KPSIGQRTRL SKGDIAQARK LYKCPACGET LQDSTGNFSS PEYPNGYSAH
360 370 380 390 400
MHCVWRISVT PGEKIILNFT SMDLYRSRLC WYDYVEVRDG FWRKAPLRGR
410 420 430 440 450
FCGGKLPEPI VSTDSRLWVE FRSSSNWVGK GFFAVYEAIC GGDVKKDNGH
460 470 480 490 500
IQSPNYPDDY RPSKVCIWRI QVSEGFHVGL TFQSFEIERH DSCAYDYLEV
510 520 530 540 550
RDGHSESSNL IGRYCGYEKP DDIKSTSSRL WLKFVSDGSI NKAGFAVNFF
560 570 580 590 600
KEVDECSRPN RGGCEQRCLN TLGSYKCSCD PGYELAPDKR RCEAACGGFL
610 620 630 640 650
TKLNGSITSP GWPKEYPPNK NCIWQLVAPT QYRISLQFDF FETEGNDVCK
660 670 680 690 700
YDFVEVRSGL TADSKLHGKF CGSEKPEVIT SQYNNMRVEF KSDNTVSKKG
710 720 730 740 750
FKAHFFSDKD ECSKDNGGCQ QDCVNTFGSY ECQCRSGFVL HDNKHDCKEA
760 770 780 790 800
GCEHKVTSTS GTITSPNWPD KYPSKKECTW AISSTPGHRV KLTFVEMDIE
810 820 830 840 850
SQPECAYDHL EVFDGRDAKA PVLGRFCGSK KPEPVLATGN RMFLRFYSDN
860 870 880 890 900
SVQRKGFQAS HSTECGGQVR ADVKTKDLYS HAQFGDNNYP GGVDCEWVIV
910 920 930 940 950
AEEGYGVELV FQTFEVEEET DCGYDYIELF DGYDSTAPRL GRYCGSGPPE
960 970 980 990 991
EVYSAGDSVL VKFHSDDTIS KKGFHLRYTS TKFQDTLHSR K

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3562 Collapse assay details

Data source: Panorama

Official Gene Symbol
Bmp1
Peptide Sequence
FYSDNSVQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
846
Peptide End
854
CPTAC ID
non-CPTAC-3562
Peptide Molecular Mass
1,114.5043
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 12.7 3.1 5.1 14 10.2 7.5 18.9 10.7 9.1 15 15 15
y5 (1+) 16.4 10.8 9 28 14.5 9.3 32.4 18.1 12.9 15 15 15
y6 (1+) 22.6 14.1 9.4 25.5 15.7 13.7 34.1 21.1 16.6 15 15 15
y7 (1+) 10.4 6.3 7.8 10.9 8.5 11.3 15.1 10.6 13.7 15 15 15
y8 (1+) 27.7 8.7 7 26.7 11.3 8 38.5 14.3 10.6 15 15 15
sum 5.5 2.9 5 8.9 7.7 7.3 10.5 8.2 8.8 15 15 15


Additional Resources and Comments