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Overview Data source: UniProt

Official Gene Symbol Other Aliases
B4galt1 Ggtb, Ggtb2
Sequence Length (AA) Molecular Weight (Da)
399 44411
Protein Name
Beta-1,4-galactosyltransferase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRFREQFLGG SAAMPGATLQ RACRLLVAVC ALHLGVTLVY YLSGRDLSRL
60 70 80 90 100
PQLVGVSSTL QGGTNGAAAS KQPPGEQRPR GARPPPPLGV SPKPRPGLDS
110 120 130 140 150
SPGAASGPGL KSNLSSLPVP TTTGLLSLPA CPEESPLLVG PMLIDFNIAV
160 170 180 190 200
DLELLAKKNP EIKTGGRYSP KDCVSPHKVA IIIPFRNRQE HLKYWLYYLH
210 220 230 240 250
PILQRQQLDY GIYVINQAGD TMFNRAKLLN IGFQEALKDY DYNCFVFSDV
260 270 280 290 300
DLIPMDDRNA YRCFSQPRHI SVAMDKFGFS LPYVQYFGGV SALSKQQFLA
310 320 330 340 350
INGFPNNYWG WGGEDDDIFN RLVHKGMSIS RPNAVVGRCR MIRHSRDKKN
360 370 380 390 399
EPNPQRFDRI AHTKETMRFD GLNSLTYKVL DVQRYPLYTQ ITVDIGTPR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3561 Collapse assay details

Data source: Panorama

Official Gene Symbol
B4galt1
Peptide Sequence
FDGLNSLTYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
369
Peptide End
378
CPTAC ID
non-CPTAC-3561
Peptide Molecular Mass
1,156.5764
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (2+) 21.4 7.5 4.6 32 8.5 6.8 38.5 11.3 8.2 15 15 15
b3 (1+) 13 6.7 3.6 19 8.6 7.3 23 10.9 8.1 15 15 15
b2 (1+) 15.6 5 3.7 16.9 11.4 5.8 23 12.4 6.9 15 15 15
y6 (1+) 22 7.7 5.1 23.3 8 8.1 32 11.1 9.6 15 15 15
y8 (1+) 7.9 5 4.3 12.3 8.5 7.6 14.6 9.9 8.7 15 15 15
sum 4.6 3 2.4 4.9 6.7 6 6.7 7.3 6.5 15 15 15


Additional Resources and Comments