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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Adamtsl4 Tsrc1
Sequence Length (AA) Molecular Weight (Da)
1036 113225
Protein Name
ADAMTS-like protein 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MESWLGRLWL CMMLLLPLPQ PCQDQELFGP SHQLPSEEGQ VPEGLWGPWG
60 70 80 90 100
RWASCSQPCG VGVQRRSRTC ELHPALPLPP RPPRHPEAHR PRGQGSRPQT
110 120 130 140 150
PRDPQSLYRP QPRGRGGPLR APASQVGREE TQEPQGAQRF RVRDPIKPGM
160 170 180 190 200
FGYGRVPFAL PLHRSRRHPH RPGQPKNSST GEGMVPSQPP STELASEKHG
210 220 230 240 250
PHMQPPEPRS HSAETPRSGT AQTEVLPRTS SAPSYTGTPA PTSSFGDSRS
260 270 280 290 300
FQGSLGPRMP PSPGSWSSPQ GAERRHPPPF SPVPRSQQSR RHWRPPGPHR
310 320 330 340 350
SPDGWLPLTR DSSPLWSIFA PSIPAPNCSG ESEQMRACSQ EPCPPEQPDP
360 370 380 390 400
RALQCAAFDS QEFMGQLYQW EPFTEVQGSQ RCELNCRPRG FRFYVRHTEK
410 420 430 440 450
VQDGTLCQPG SLDICVAGRC LSPGCDGVLG SGRRPDGCGV CGGDGSTCRL
460 470 480 490 500
VSGNLTDRGG PLGYQKILWI PAGASHLHIS QLRPSSNYLA LRGPGGRSII
510 520 530 540 550
NGNWAVDPPG SYTAIGTVFQ YNRPPREEGK GESLSAEGPT TQPVDVYMIF
560 570 580 590 600
QEDNPGVFYQ YVISSPPAVL ESPSTKPPAL QPQPEMLRGE PLLPSAPRPV
610 620 630 640 650
RAPGTLQRQV RIPQVPPPTR VRTAMGSSAG YWKQVGHSEC SASCGKGVWH
660 670 680 690 700
PIFLCISRES GEELDEQSCA VGARPPASPE PCHGPPCPPY WEAGEWTSCS
710 720 730 740 750
RSCGPGTQHR QLLCRQEFGG GGSSVPPERC GHLPRPNITQ PCQLHLCGHW
760 770 780 790 800
EISSPWSQCS VRCGRGQRSR QVRCVGSNGD EVDKQECASG PPPPPSREAC
810 820 830 840 850
DMGPCTTAWF YSDWSSKCSA ECGTGIQRRA VVCLRSGETL QGDPEAGSTE
860 870 880 890 900
QGCPLRSRPP DMRACSLGPC ERTWRWFTGP WSECSSECGS GTQHRDIICV
910 920 930 940 950
SKLGAEFNVT SPSNCSHLPR PPALQPCQGQ ACEDKWFSTL WSPCSRSCQG
960 970 980 990 1000
GMQTREVQCL SGNQTLSSRC PPHLRPSRKR PCNSQPCNQR PDDQCKDSSP
1010 1020 1030 1036
HCPLVVQARL CVYPYYTTTC CRSCAHVLEQ SQLEPA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3560 Collapse assay details

Data source: Panorama

Official Gene Symbol
Adamtsl4
Peptide Sequence
GGPLGYQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
459
Peptide End
466
CPTAC ID
non-CPTAC-3560
Peptide Molecular Mass
818.4287
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 11.1 6.4 9.3 16.2 8.2 10.2 19.6 10.4 13.8 15 15 15
y6 (2+) 6.4 3.8 8 10.5 12.4 8.5 12.3 13 11.7 15 15 15
y4 (1+) 11.6 7.5 9.1 11.1 10 12.1 16.1 12.5 15.1 15 15 15
y5 (1+) 15.8 7 11.3 21 11.6 12.3 26.3 13.5 16.7 15 15 15
y6 (1+) 20.1 19.6 7.1 20.6 22.7 12.9 28.8 30 14.7 15 15 15
sum 5.7 2.7 4.7 9.5 10 6.6 11.1 10.4 8.1 15 15 15


Additional Resources and Comments