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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Kiaa0319l Aavr
Sequence Length (AA) Molecular Weight (Da)
1048 115312
Protein Name
Dyslexia-associated protein KIAA0319-like protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEKRLGVKPS PASWVLPGYC WQTSVKLPRS LYLLYSFFCF SVLWLSTDAD
60 70 80 90 100
ESRCQQGKTL YGAGLRTEGE NHLRLLAGSL PFHACRAACC RDSACHALWW
110 120 130 140 150
LEGMCFQADC SKPQSCQPFR TDSSNSMLII FQKSQTTDDL GLLPEDDEPH
160 170 180 190 200
LLRLGWGRTS WRRQSLLGAP LTLSVPSSHH QSLLRDRQKR DLSVVPTHGA
210 220 230 240 250
MQHSKVNHSE EAGALSPTSA EVRKTITVAG SFTSNHTTQT PEWPKNVSIH
260 270 280 290 300
PEPSEHSSPV SGTPQVKSTE HSPTDAPLPV APSYSYATPT PQASSQSTSA
310 320 330 340 350
PHPVVKELVV SAGKSVQITL PKNEVQLNAF VLPEAEPGET YTYDWQLITH
360 370 380 390 400
PTDYSGEVER KHSQSLQLSK LTPGLYEFKV TVDGQNAHGE GYVNVTVKPE
410 420 430 440 450
PRKNRPPVAV VSPQFQEISL PTTSTIIDGS QSTDDDKIVQ YHWEELKGPL
460 470 480 490 500
REEKISEDTA ILKLSKLVPG NYTFSLTVVD SDGATNSTTA SLTVNKAVDY
510 520 530 540 550
PPVANAGPNQ VITLPQNSIT LFGNQSTDDH GITSYEWSLS PSSKGKVVEM
560 570 580 590 600
QGVRTPALQL SAMQEGDYTY QLTVTDTAGQ QATAQVTVIV QPENNKPPQA
610 620 630 640 650
DAGPDKELTL PVDSTTLDGS KSTDDQRVVS YLWEQSRGPD GVQLENANSS
660 670 680 690 700
VATVTGLQVG TYVFTLTVKD ERNLQSQSSV NVIVKEEINK PPVAKIAGNV
710 720 730 740 750
VVTLPTSTAE LDGSRSSDDK GIVSYLWTRD ETSPAAGEVL NHSDHHPVLF
760 770 780 790 800
LSNLVEGTYT FHLKVTDAKG ESDTDRTTVE VKPDPRKSNL VEIILDVNVS
810 820 830 840 850
QLTERLKGML IRQIGVLLGV LDSDIIVQKI QPYTEQSTKM LFFVQNDPPH
860 870 880 890 900
QLFKGHEVAA MLKSELQKQK ADFLIFRALE ISTVTCQLNC SDHGHCDSFT
910 920 930 940 950
KRCVCDPFWM ENFIKVQLRD GDSNCEWSVL YVIIASFVIV VALGILSWTT
960 970 980 990 1000
ICCCKRQKGK PKRKSRYKIL DATDQESLEL KPTSRAGSKQ KGPTLSSSLM
1010 1020 1030 1040 1048
HSESELDSDD AIFTWPDREK GKLLYGQNGS VPNGQTPLKS RSAREEIL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3586 Collapse assay details

Data source: Panorama

Official Gene Symbol
Kiaa0319l
Peptide Sequence
ISEDTAILK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
455
Peptide End
463
CPTAC ID
non-CPTAC-3586
Peptide Molecular Mass
988.5441
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 17.1 4.2 5 18.4 6.2 6.6 25.1 7.5 8.3 15 15 15
b2 (1+) 43.7 4.2 4 49.4 8.8 5.9 66 9.8 7.1 15 15 15
y2 (1+) 20.9 4.7 4 29.8 7.2 4.9 36.4 8.6 6.3 15 15 15
y6 (1+) 33 7.2 5.8 35.6 7.4 7.1 48.5 10.3 9.2 15 15 15
y7 (1+) 10.5 5.4 4.2 13 8.1 5.4 16.7 9.7 6.8 15 15 15
sum 16.8 2.1 2.1 19.4 6.2 4.5 25.7 6.5 5 15 15 15


Additional Resources and Comments