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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Selp Grmp
Sequence Length (AA) Molecular Weight (Da)
768 83099
Protein Name
P-selectin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGCPKGSWT PRLRSVILGG AQLIWFSALI SELVNQKEVA AWTYNYSTKA
60 70 80 90 100
YSWNNSRVFC RRHFTDLVAI QNKNEIAHLN DVIPFFNSYY WIGIRKINNK
110 120 130 140 150
WTWVGTNKTL TEEAENWADN EPNNKKNNQD CVEIYIKSNS APGKWNDEPC
160 170 180 190 200
FKRKRALCYT ASCQDMSCSN QGECIETIGS YTCSCYPGFY GPECEYVKEC
210 220 230 240 250
GKVNIPQHVL MNCSHPLGEF SFNSQCTFSC AEGYELDGPG ELQCLASGIW
260 270 280 290 300
TNNPPKCDAV QCQSLEAPPH GTMACMHPIA AFAYDSSCKF ECQPGYRARG
310 320 330 340 350
SNTLHCTGSG QWSEPLPTCE AIACEPPEIP IHGSMDCVPS TGTFGYNSSC
360 370 380 390 400
TFLCAEGFVL KGNDAIQCAD SGQWTAPAPF CEALQCPEFP VPSKAQVNCS
410 420 430 440 450
DPFGTLTYQS VCSFSCDEGS LLVGASVIRC LATGHWNGAP PECQAVSCAP
460 470 480 490 500
MLSPENGSMT CVQPLGNSTY KSTCQFMCDE GFYLSGPERL DCSPSGHWTG
510 520 530 540 550
TPPTCEAIKC PGIFAPEQGN LDCSHVHGEF GVGSICHFSC NEDFELLGSE
560 570 580 590 600
NVECTVSGRW SAPPPTCKGI TSLPAPAVRC PALTTPGQGT MSCQHHLGSF
610 620 630 640 650
GPNTTCYFGC KTGFTLRGAN SLRCRASGQW TAVTPMCRAV KCSELHMDTA
660 670 680 690 700
VAMNCSNPWG NFSYGSTCTF QCPEGQSLNG SVRATCREDG HWSDAMPTCQ
710 720 730 740 750
AGTLTIQEAL TYLGGAVAST TGLAVGGTLL ALLRKRLRKK DDGKCPLNPH
760 768
SHLGTYGVFT NAAYDPTP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3590 Collapse assay details

Data source: Panorama

Official Gene Symbol
Selp
Peptide Sequence
GITSLPAPAVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
569
Peptide End
579
CPTAC ID
non-CPTAC-3590
Peptide Molecular Mass
1,080.6291
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b10 (2+) 27.6 7.7 5.1 29.1 14 8.4 40.1 16 9.8 15 15 15
b5 (1+) 18.5 10.2 6.1 20.9 11.7 7.2 27.9 15.5 9.4 15 15 15
y9 (2+) 50.2 25 8.2 48.6 23.5 15.9 69.9 34.3 17.9 15 15 15
y4 (1+) 28 16.9 7 28.8 31 9.8 40.2 35.3 12 15 15 15
y6 (1+) 13.9 4.1 4.1 10.1 6.7 7.4 17.2 7.9 8.5 15 15 15
sum 8.2 3 3.8 11 6.9 7.2 13.7 7.5 8.1 15 15 15


Additional Resources and Comments