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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Man2b1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Man2b1 Laman, Man2b, Manb
Sequence Length (AA) Molecular Weight (Da)
1013 114648
Protein Name
Lysosomal alpha-mannosidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGTGPLTSGV RAGGGNTGWL WMSSCNLGSP VLPISFLFWL LLAAPGARAA
60 70 80 90 100
GYKTCPPTKP GMLNVHLLPH THDDVGWLKT VDQYYYGILS DVQHASVQYI
110 120 130 140 150
LDSVVSSLLE KPTRRFIYVE MAFFSRWWKQ QTSATQDAVR NLVRQGRLEF
160 170 180 190 200
VNGGWVMNDE AATHYGAIVD QMTLGLRFLQ DTFGSDGLPR VAWHIDPFGH
210 220 230 240 250
SREQASLFAQ MGFDGFFLGR IDYQDKLNRK KKLRMEELWR ASDSLEPPAA
260 270 280 290 300
DLFTGVLPNN YNPPKYLCWD VLCTDPPVVD NPRSPEFNAK TLVNYFLKLA
310 320 330 340 350
SSQKGFYRTN HTVMTMGSDF HYENANMWFK NMDKLIRLVN AQQVNGSLVH
360 370 380 390 400
VLYSTPTCYL WELNKANLTW TVKEDDFFPY ADGPHMFWTG YFSSRPALKR
410 420 430 440 450
YERLSYNFLQ VCNQLEALVG PEANVGPYGS GDSAPLQEAM AVLQHHDAVS
460 470 480 490 500
GTARQNVVND YARQLAAGWG PCEVLVSNAL ARLSHYKQNF SFCRELNISI
510 520 530 540 550
CPVSQTSERF QVTLYNPLGR KVDQMVRLPV YEGNFIVKDP HDKNISSNVV
560 570 580 590 600
MVPSYYSETY QWELLFPASV PALGFSTYSV AKMSDLNHQA HNLLSRPRKH
610 620 630 640 650
KSHHVLVIEN KYMRATFDSG TGLLMKIENL EQNLSLPVSQ GFFWYNASVG
660 670 680 690 700
DEESSQASGA YIFRPNVGKP IPVSRWAQIS LVKTALVQEV HQNFSAWCSQ
710 720 730 740 750
VIRLYKGQRH LELEWTVGPI PVRDDWGKEV ISRFDTPMKT KGQFFTDSNG
760 770 780 790 800
REILKRRDDY RPTWTLNQTE PVAGNYYPVN TRIYITDGQM QLTVLTDRSQ
810 820 830 840 850
GGSSLQDGSL ELMVHRRLLV DDDRGVSEPL LETDTGDKVR GRHLVLLSSV
860 870 880 890 900
SDAAARHRLL AEQEVLAPQV VLSLGGSSPY HSRATPKTQF SGLRQELPPQ
910 920 930 940 950
VHLLTLARWG PKMLLLRLEH QFALKEDSDR NLSSPVTLNV QNLFQTFTIN
960 970 980 990 1000
YLQETTLAAN QPLSRASRLK WMTNTGPTSY PEPSKLDPTS VTLKPMEIRT
1010 1013
FLASVQWQEH RPA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2225 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAN2B1
Peptide Modified Sequence
TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK
Modification Type
Deamidated (N), Oxidation (M), Oxidation (M), Oxidation (M)
Protein - Site of Modification
310, 314, 316, 327
Peptide - Site of Modification
2, 6, 8, 19
Peptide Start
309
Peptide End
330
CPTAC ID
CPTAC-2225
Peptide Molecular Mass
2,700.0931
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 26.3 8.2 10.3 24.9 9.3 11 36.2 12.4 15.1 15 15 15
y8 (1+) 22 11.6 7.3 19.8 9.5 6.3 29.6 15 9.6 15 15 15
y9 (1+) 19.4 8.1 12.2 22.2 7.5 8.4 29.5 11 14.8 15 15 15
sum 15.5 6.2 7.4 14.4 5.2 6.1 21.2 8.1 9.6 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-3591 Collapse assay details

Data source: Panorama

Official Gene Symbol
Man2b1
Peptide Sequence
GVSEPLLETDTGDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
825
Peptide End
838
CPTAC ID
non-CPTAC-3591
Peptide Molecular Mass
1,459.7042
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 19 5.7 5 17.5 8.1 9.9 25.8 9.9 11.1 15 15 15
b3 (1+) 20.1 5.3 3.7 24.5 8.1 13 31.7 9.7 13.5 15 15 15
y10 (2+) 10.7 6 4.7 12.7 8.2 6.7 16.6 10.2 8.2 15 15 15
y8 (1+) 9.1 6.1 7.4 8.2 8.7 7.5 12.2 10.6 10.5 15 15 15
y10 (1+) 5.3 5 5.2 7.2 9.2 8.2 8.9 10.5 9.7 15 15 15
sum 4.3 1.5 2.6 6.5 6.6 6.9 7.8 6.8 7.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments