Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Spp1 Eta-1, Op, Spp-1
Sequence Length (AA) Molecular Weight (Da)
294 32459
Protein Name
Osteopontin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLAVICFCL FGIASSLPVK VTDSGSSEEK LYSLHPDPIA TWLVPDPSQK
60 70 80 90 100
QNLLAPQNAV SSEEKDDFKQ ETLPSNSNES HDHMDDDDDD DDDDGDHAES
110 120 130 140 150
EDSVDSDESD ESHHSDESDE TVTASTQADT FTPIVPTVDV PNGRGDSLAY
160 170 180 190 200
GLRSKSRSFQ VSDEQYPDAT DEDLTSHMKS GESKESLDVI PVAQLLSMPS
210 220 230 240 250
DQDNNGKGSH ESSQLDEPSL ETHRLEHSKE SQESADQSDV IDSQASSKAS
260 270 280 290 294
LEHQSHKFHS HKDKLVLDPK SKEDDRYLKF RISHELESSS SEVN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-3592 Collapse assay details

Data source: Panorama

Official Gene Symbol
Spp1
Peptide Sequence
GDSLAYGLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
145
Peptide End
153
CPTAC ID
non-CPTAC-3592
Peptide Molecular Mass
950.4821
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.2 4 4.4 15 4.2 8.3 15.9 5.8 9.4 15 15 15
b5 (1+) 10.7 16 14 15.2 16.9 17.3 18.6 23.3 22.3 15 15 15
y7 (2+) 12.4 15 12.5 18.1 15.8 13.9 21.9 21.8 18.7 15 15 15
y3 (1+) 9.5 7.8 5.4 12.1 11.1 31.6 15.4 13.6 32.1 15 15 15
y4 (1+) 4.4 4.4 6.5 12.4 8 11.2 13.2 9.1 12.9 15 15 15
sum 3.4 2.5 4 11.2 3.9 12.8 11.7 4.6 13.4 15 15 15

Additional Resources and Comments