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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Psma1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Psma1 N/A
Sequence Length (AA) Molecular Weight (Da)
263 29547
Protein Name
Proteasome subunit alpha type-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MFRNQYDNDV TVWSPQGRIH QIEYAMEAVK QGSATVGLKS KTHAVLVALK
60 70 80 90 100
RAQSELAAHQ KKILHVDNHI GISIAGLTAD ARLLCNFMRQ ECLDSRFVFD
110 120 130 140 150
RPLPVSRLVS LIGSKTQIPT QRYGRRPYGV GLLIAGYDDM GPHIFQTCPS
160 170 180 190 200
ANYFDCRAMS IGARSQSART YLERHMSEFM ECNLDELVKH GLRALRETLP
210 220 230 240 250
AEQDLTTKNV SIGIVGKDLE FTIYDDDDVS PFLDGLEERP QRKAQPSQAA
260 263
EEPAEKADEP MEH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5955 Collapse assay details

Data source: Panorama

Official Gene Symbol
PSMA1
Peptide Sequence
ETLPAEQDLTTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
4
Peptide Start
197
Peptide End
208
CPTAC ID
CPTAC-5955
Peptide Molecular Mass
1,344.6773
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
FFPE tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 12.7 7.4 4.1 14.9 11.6 10.2 19.6 13.8 11 13 25 14
b3 (1+) 10.8 5.7 4.4 12.4 10.5 10.3 16.4 11.9 11.2 13 25 14
y7 (1+) 6.1 6.5 3.2 10.3 9.2 10 12 11.3 10.5 13 25 14
y8 (1+) 8.9 5.8 2.8 10 11.1 8.8 13.4 12.5 9.2 13 25 14
y9 (1+) 6.5 5.7 1.6 11 9.5 9.8 12.8 11.1 9.9 13 25 14
y6 (1+) 5.7 5.9 5.2 12.4 11.9 10.3 13.6 13.3 11.5 13 25 14
sum 5.9 4.8 2 10.3 9.5 9.7 11.9 10.6 9.9 13 25 14

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for non-CPTAC-3602 Collapse assay details

Data source: Panorama

Official Gene Symbol
Psma1
Peptide Sequence
ETLPAEQDLTTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
197
Peptide End
208
CPTAC ID
non-CPTAC-3602
Peptide Molecular Mass
1,344.6773
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 17.2 8.1 3.9 23.7 8.8 5.1 29.3 12 6.4 15 15 15
b2 (1+) 36.1 7.7 5.9 53.1 8.8 6.1 64.2 11.7 8.5 15 15 15
y9 (2+) 10.2 4.4 2.7 11.3 5.3 5 15.2 6.9 5.7 15 15 15
y10 (2+) 48.8 7.9 7.6 52.9 13.4 8.2 72 15.6 11.2 15 15 15
y9 (1+) 8.4 5.1 2.3 9.3 6.6 4.7 12.5 8.3 5.2 15 15 15
sum 7.1 2.2 1.4 9.3 4.6 3.8 11.7 5.1 4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments