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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Pzp

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Pzp A2m
Sequence Length (AA) Molecular Weight (Da)
1495 165853
Protein Name
Pregnancy zone protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRRNQLPTPA FLLLFLLLPR DATTATAKPQ YVVLVPSEVY SGVPEKACVS
60 70 80 90 100
LNHVNETVML SLTLEYAMQQ TKLLTDQAVD KDSFYCSPFT ISGSPLPYTF
110 120 130 140 150
ITVEIKGPTQ RFIKKKSIQI IKAESPVFVQ TDKPIYKPGQ IVKFRVVSVD
160 170 180 190 200
ISFRPLNETF PVVYIETPKR NRIFQWQNIH LAGGLHQLSF PLSVEPALGI
210 220 230 240 250
YKVVVQKDSG KKIEHSFEVK EYVLPKFEVI IKMQKTMAFL EEELPITACG
260 270 280 290 300
VYTYGKPVPG LVTLRVCRKY SRYRSTCHNQ NSMSICEEFS QQADDKGCFR
310 320 330 340 350
QVVKTKVFQL RQKGHDMKIE VEAKIKEEGT GIELTGIGSC EIANALSKLK
360 370 380 390 400
FTKVNTNYRP GLPFSGQVLL VDEKGKPIPN KNITSVVSPL GYLSIFTTDE
410 420 430 440 450
HGLANISIDT SNFTAPFLRV VVTYKQNHVC YDNWWLDEFH TQADHSATLV
460 470 480 490 500
FSPSQSYIQL ELVFGTLACG QTQEIRIHYL LNEDIMKNEK DLTFYYLIKA
510 520 530 540 550
RGSIFNLGSH VLSLEQGNMK GVFSLPIQVE PGMAPEAQLL IYAILPNEEL
560 570 580 590 600
VADAQNFEIE KCFANKVNLS FPSAQSLPAS DTHLKVKAAP LSLCALTAVD
610 620 630 640 650
QSVLLLKPEA KLSPQSIYNL LPGKTVQGAF FGVPVYKDHE NCISGEDITH
660 670 680 690 700
NGIVYTPKHS LGDNDAHSIF QSVGINIFTN SKIHKPRFCQ EFQHYPAMGG
710 720 730 740 750
VAPQALAVAA SGPGSSFRAM GVPMMGLDYS DEINQVVEVR ETVRKYFPET
760 770 780 790 800
WIWDLVPLDV SGDGELAVKV PDTITEWKAS AFCLSGTTGL GLSSTISLQA
810 820 830 840 850
FQPFFLELTL PYSVVRGEAF TLKATVLNYM SHCIQIRVDL EISPDFLAVP
860 870 880 890 900
VGGHENSHCI CGNERKTVSW AVTPKSLGEV NFTATAEALQ SPELCGNKLT
910 920 930 940 950
EVPALVHKDT VVKSVIVEPE GIEKEQTYNT LLCPQDTELQ DNWSLELPPN
960 970 980 990 1000
VVEGSARATH SVLGDILGSA MQNLQNLLQM PYGCGEQNMV LFVPNIYVLN
1010 1020 1030 1040 1050
YLNETQQLTE AIKSKAINYL ISGYQRQLNY QHSDGSYSTF GNHGGGNTPG
1060 1070 1080 1090 1100
NTWLTAFVLK AFAQAQSHIF IEKTHITNAF NWLSMKQKEN GCFQQSGYLL
1110 1120 1130 1140 1150
NNAMKGGVDD EVTLSAYITI ALLEMPLPVT HSAVRNALFC LETAWASISQ
1160 1170 1180 1190 1200
SQESHVYTKA LLAYAFALAG NKAKRSELLE SLNKDAVKEE DSLHWQRPGD
1210 1220 1230 1240 1250
VQKVKALSFY QPRAPSAEVE MTAYVLLAYL TSESSRPTRD LSSSDLSTAS
1260 1270 1280 1290 1300
KIVKWISKQQ NSHGGFSSTQ DTVVALQALS KYGAATFTRS QKEVLVTIES
1310 1320 1330 1340 1350
SGTFSKTFHV NSGNRLLLQE VRLPDLPGNY VTKGSGSGCV YLQTSLKYNI
1360 1370 1380 1390 1400
LPVADGKAPF ALQVNTLPLN FDKAGDHRTF QIRINVSYTG ERPSSNMVIV
1410 1420 1430 1440 1450
DVKMVSGFIP MKPSVKKLQD QPNIQRTEVN TNHVLIYIEK LTNQTLGFSF
1460 1470 1480 1490 1495
AVEQDIPVKN LKPAPIKVYD YYETDEFTVE EYSAPFSDGS EQGNA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2689 Collapse assay details

Data source: Panorama

Official Gene Symbol
PZP
Peptide Sequence
AVGYLITGYQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1010
Peptide End
1020
CPTAC ID
non-CPTAC-2689
Peptide Molecular Mass
1,239.6612
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 10.6 7.2 8.3 10.9 5.9 9.4 15.2 9.3 12.5 15 15 15
y4 (1+) 19.2 6.1 7.8 25.2 11.4 9.4 31.7 12.9 12.2 15 15 15
y6 (1+) 9 4.7 6.7 16.2 6.7 8.9 18.5 8.2 11.1 15 15 15
y5 (1+) 9.8 4.9 6.4 19.8 13.8 10.1 22.1 14.6 12 15 15 15
sum 4.6 1.5 5.9 11.7 8 8.4 12.6 8.1 10.3 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-2688 Collapse assay details

Data source: Panorama

Official Gene Symbol
PZP
Peptide Sequence
ISEITNIVSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
336
Peptide End
345
CPTAC ID
non-CPTAC-2688
Peptide Molecular Mass
1,102.6234
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 14.4 5.6 5 16.8 6.7 7.6 22.1 8.7 9.1 15 15 15
b4 (1+) 17.3 9.2 4.5 21.2 10.6 8.7 27.4 14 9.8 15 15 15
y6 (1+) 11.9 4.9 7.2 14.9 10.9 6.8 19.1 12 9.9 15 15 15
y3 (1+) 21.7 6.3 6.6 38.3 11.7 7.1 44 13.3 9.7 15 15 15
b3 (1+) 18.3 5.5 5 16.2 7.4 7 24.4 9.2 8.6 15 15 15
sum 8 3.2 5.1 11.8 7.5 6.4 14.3 8.2 8.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3606 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pzp
Peptide Sequence
LSPQSIYNLLPGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
612
Peptide End
624
CPTAC ID
non-CPTAC-3606
Peptide Molecular Mass
1,428.7977
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 1.6 2.2 1.5 7.2 12.3 17.5 7.4 12.5 17.6 15 15 15
y3 (1+) 1.1 2.6 1.2 4.8 13.1 15.7 4.9 13.4 15.7 15 15 15
y4 (1+) 3.6 1.2 1.2 19.3 13.6 15.1 19.6 13.7 15.1 15 15 15
y7 (1+) 2.5 1.4 1.6 4.9 13.3 15.2 5.5 13.4 15.3 15 15 15
b10 (1+) 2.4 1.8 2.3 6 12.6 15 6.5 12.7 15.2 15 15 15
sum 0.9 1.4 1 6.3 12.4 16.4 6.4 12.5 16.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments