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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PZP

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PZP CPAMD6
Sequence Length (AA) Molecular Weight (Da)
1482 163863
Protein Name
Pregnancy zone protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRKDRLLHLC LVLLLILLSA SDSNSTEPQY MVLVPSLLHT EAPKKGCVLL
60 70 80 90 100
SHLNETVTVS ASLESGRENR SLFTDLVAEK DLFHCVSFTL PRISASSEVA
110 120 130 140 150
FLSIQIKGPT QDFRKRNTVL VLNTQSLVFV QTDKPMYKPG QTVRFRVVSV
160 170 180 190 200
DENFRPRNEL IPLIYLENPR RNRIAQWQSL KLEAGINQLS FPLSSEPIQG
210 220 230 240 250
SYRVVVQTES GGRIQHPFTV EEFVLPKFEV KVQVPKIISI MDEKVNITVC
260 270 280 290 300
GEYTYGKPVP GLATVSLCRK LSRVLNCDKQ EVCEEFSQQL NSNGCITQQV
310 320 330 340 350
HTKMLQITNT GFEMKLRVEA RIREEGTDLE VTANRISEIT NIVSKLKFVK
360 370 380 390 400
VDSHFRQGIP FFAQVLLVDG KGVPIPNKLF FISVNDANYY SNATTNEQGL
410 420 430 440 450
AQFSINTTSI SVNKLFVRVF TVHPNLCFHY SWVAEDHQGA QHTANRVFSL
460 470 480 490 500
SGSYIHLEPV AGTLPCGHTE TITAHYTLNR QAMGELSELS FHYLIMAKGV
510 520 530 540 550
IVRSGTHTLP VESGDMKGSF ALSFPVESDV APIARMFIFA ILPDGEVVGD
560 570 580 590 600
SEKFEIENCL ANKVDLSFSP AQSPPASHAH LQVAAAPQSL CALRAVDQSV
610 620 630 640 650
LLMKPEAELS VSSVYNLLTV KDLTNFPDNV DQQEEEQGHC PRPFFIHNGA
660 670 680 690 700
IYVPLSSNEA DIYSFLKGMG LKVFTNSKIR KPKSCSVIPS VSAGAVGQGY
710 720 730 740 750
YGAGLGVVER PYVPQLGTYN VIPLNNEQSS GPVPETVRSY FPETWIWELV
760 770 780 790 800
AVNSSGVAEV GVTVPDTITE WKAGAFCLSE DAGLGISSTA SLRAFQPFFV
810 820 830 840 850
ELTMPYSVIR GEVFTLKATV LNYLPKCIRV SVQLKASPAF LASQNTKGEE
860 870 880 890 900
SYCICGNERQ TLSWTVTPKT LGNVNFSVSA EAMQSLELCG NEVVEVPEIK
910 920 930 940 950
RKDTVIKTLL VEAEGIEQEK TFSSMTCASG ANVSEQLSLK LPSNVVKESA
960 970 980 990 1000
RASFSVLGDI LGSAMQNIQN LLQMPYGCGE QNMVLFAPNI YVLNYLNETQ
1010 1020 1030 1040 1050
QLTQEIKAKA VGYLITGYQR QLNYKHQDGS YSTFGERYGR NQGNTWLTAF
1060 1070 1080 1090 1100
VLKTFAQARS YIFIDEAHIT QSLTWLSQMQ KDNGCFRSSG SLLNNAIKGG
1110 1120 1130 1140 1150
VEDEATLSAY VTIALLEIPL PVTNPIVRNA LFCLESAWNV AKEGTHGSHV
1160 1170 1180 1190 1200
YTKALLAYAF SLLGKQNQNR EILNSLDKEA VKEDNLVHWE RPQRPKAPVG
1210 1220 1230 1240 1250
HLYQTQAPSA EVEMTSYVLL AYLTAQPAPT SGDLTSATNI VKWIMKQQNA
1260 1270 1280 1290 1300
QGGFSSTQDT VVALHALSRY GAATFTRTEK TAQVTVQDSQ TFSTNFQVDN
1310 1320 1330 1340 1350
NNLLLLQQIS LPELPGEYVI TVTGERCVYL QTSMKYNILP EKEDSPFALK
1360 1370 1380 1390 1400
VQTVPQTCDG HKAHTSFQIS LTISYTGNRP ASNMVIVDVK MVSGFIPLKP
1410 1420 1430 1440 1450
TVKMLERSSS VSRTEVSNNH VLIYVEQVTN QTLSFSFMVL QDIPVGDLKP
1460 1470 1480 1482
AIVKVYDYYE TDESVVAEYI APCSTDTEHG NV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2689 Collapse assay details

Data source: Panorama

Official Gene Symbol
PZP
Peptide Sequence
AVGYLITGYQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1010
Peptide End
1020
CPTAC ID
non-CPTAC-2689
Peptide Molecular Mass
1,239.6612
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 10.6 7.2 8.3 10.9 5.9 9.4 15.2 9.3 12.5 15 15 15
y4 (1+) 19.2 6.1 7.8 25.2 11.4 9.4 31.7 12.9 12.2 15 15 15
y6 (1+) 9 4.7 6.7 16.2 6.7 8.9 18.5 8.2 11.1 15 15 15
y5 (1+) 9.8 4.9 6.4 19.8 13.8 10.1 22.1 14.6 12 15 15 15
sum 4.6 1.5 5.9 11.7 8 8.4 12.6 8.1 10.3 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-2688 Collapse assay details

Data source: Panorama

Official Gene Symbol
PZP
Peptide Sequence
ISEITNIVSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
336
Peptide End
345
CPTAC ID
non-CPTAC-2688
Peptide Molecular Mass
1,102.6234
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 14.4 5.6 5 16.8 6.7 7.6 22.1 8.7 9.1 15 15 15
b4 (1+) 17.3 9.2 4.5 21.2 10.6 8.7 27.4 14 9.8 15 15 15
y6 (1+) 11.9 4.9 7.2 14.9 10.9 6.8 19.1 12 9.9 15 15 15
y3 (1+) 21.7 6.3 6.6 38.3 11.7 7.1 44 13.3 9.7 15 15 15
b3 (1+) 18.3 5.5 5 16.2 7.4 7 24.4 9.2 8.6 15 15 15
sum 8 3.2 5.1 11.8 7.5 6.4 14.3 8.2 8.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3606 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pzp
Peptide Sequence
LSPQSIYNLLPGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
612
Peptide End
624
CPTAC ID
non-CPTAC-3606
Peptide Molecular Mass
1,428.7977
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 1.6 2.2 1.5 7.2 12.3 17.5 7.4 12.5 17.6 15 15 15
y3 (1+) 1.1 2.6 1.2 4.8 13.1 15.7 4.9 13.4 15.7 15 15 15
y4 (1+) 3.6 1.2 1.2 19.3 13.6 15.1 19.6 13.7 15.1 15 15 15
y7 (1+) 2.5 1.4 1.6 4.9 13.3 15.2 5.5 13.4 15.3 15 15 15
b10 (1+) 2.4 1.8 2.3 6 12.6 15 6.5 12.7 15.2 15 15 15
sum 0.9 1.4 1 6.3 12.4 16.4 6.4 12.5 16.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments