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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

SERPINA10

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPINA10 ZPI
Sequence Length (AA) Molecular Weight (Da)
444 50707
Protein Name
Protein Z-dependent protease inhibitor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKVVPSLLLS VLLAQVWLVP GLAPSPQSPE TPAPQNQTSR VVQAPKEEEE
60 70 80 90 100
DEQEASEEKA SEEEKAWLMA SRQQLAKETS NFGFSLLRKI SMRHDGNMVF
110 120 130 140 150
SPFGMSLAMT GLMLGATGPT ETQIKRGLHL QALKPTKPGL LPSLFKGLRE
160 170 180 190 200
TLSRNLELGL TQGSFAFIHK DFDVKETFFN LSKRYFDTEC VPMNFRNASQ
210 220 230 240 250
AKRLMNHYIN KETRGKIPKL FDEINPETKL ILVDYILFKG KWLTPFDPVF
260 270 280 290 300
TEVDTFHLDK YKTIKVPMMY GAGKFASTFD KNFRCHVLKL PYQGNATMLV
310 320 330 340 350
VLMEKMGDHL ALEDYLTTDL VETWLRNMKT RNMEVFFPKF KLDQKYEMHE
360 370 380 390 400
LLRQMGIRRI FSPFADLSEL SATGRNLQVS RVLQRTVIEV DERGTEAVAG
410 420 430 440 444
ILSEITAYSM PPVIKVDRPF HFMIYEETSG MLLFLGRVVN PTLL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2690 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINA10
Peptide Sequence
ETSNFGFSLLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
78
Peptide End
88
CPTAC ID
non-CPTAC-2690
Peptide Molecular Mass
1,269.6354
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 15.3 4.8 11 15.5 4.9 11.8 21.8 6.9 16.1 15 15 15
y7 (1+) 7.6 6.2 9.9 11 9.1 13.6 13.4 11 16.8 15 15 15
y6 (1+) 6.5 6 9.2 10.1 7.6 10.8 12 9.7 14.2 15 15 15
y4 (1+) 6.8 6.1 8.3 11.1 8.8 11.5 13 10.7 14.2 15 15 15
y3 (1+) 9.8 9.3 7.5 16.7 10.6 8.6 19.4 14.1 11.4 15 15 15
sum 4.8 3.6 8.1 8.3 6.1 10.3 9.6 7.1 13.1 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-5623 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpina10
Peptide Sequence
LFDEINPETK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
224
Peptide End
233
CPTAC ID
non-CPTAC-5623
Peptide Molecular Mass
1,204.5976
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 10.4 4.3 4.3 10.6 6.6 4.8 14.8 7.9 6.4 15 15 15
b2 (1+) 7.4 2.5 4.5 10 5 4.1 12.4 5.6 6.1 15 15 15
y2 (1+) 5.3 5.2 4.9 4.9 8.2 3.9 7.2 9.7 6.3 15 15 15
y4 (1+) 3.3 3.2 2.7 7.8 6.6 3 8.5 7.3 4 15 15 15
y5 (1+) 7 3.8 4.8 6.1 4.9 5.1 9.3 6.2 7 15 15 15
sum 2.8 1.6 1.5 4.9 5.5 2.2 5.6 5.7 2.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3888 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpina10
Peptide Sequence
VLQQSVLEVDER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
386
Peptide End
397
CPTAC ID
non-CPTAC-3888
Peptide Molecular Mass
1,413.7464
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 14.7 6.7 3.9 15.3 9 7.7 21.2 11.2 8.6 15 15 15
y6 (1+) 7.5 7.3 6.2 11.3 9.4 9.9 13.6 11.9 11.7 15 15 15
y2 (1+) 9.7 6.9 3.6 13.6 8 9.6 16.7 10.6 10.3 15 15 15
y10 (2+) 15.4 8.4 6.6 17.1 11.4 9.7 23 14.2 11.7 15 15 15
b2 (1+) 15.8 4.6 5.4 14.1 9.8 9.9 21.2 10.8 11.3 15 15 15
sum 8.1 3.2 2 8.2 6.8 7.7 11.5 7.5 8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments