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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Acin1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Acin1 Acinus
Sequence Length (AA) Molecular Weight (Da)
1338 150719
Protein Name
Apoptotic chromatin condensation inducer in the nucleus
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWGRKRPNSS GETRGILSGN RGVDYGSGRG QSGPFEGRWR KLPKMPEAVG
60 70 80 90 100
TDPSTSRKMA ELEEVTLDGK PLQALRVTDL KAALEQRGLA KSGQKSALVK
110 120 130 140 150
RLKGALMLEN LQKHSTPHAA FQPNSQIGEE MSQNSFIKQY LEKQQELLRQ
160 170 180 190 200
RLEREAREAA ELEEASAESE DEMTHPEGVA SLLPPDFQSS LNRPELELST
210 220 230 240 250
HSPRKSSSFS EEKGESDDEK PRKGERRSSR VRQAKSKLPE YSQTAEEEED
260 270 280 290 300
QETPSRNLRV RADRNLKIEE EEEEEEEEED DDDEEEEEVD EAQKSREAEA
310 320 330 340 350
PTLKQFEDEE GEERTRAKPE KVVDEKPLNI RSQEKGELEK GGRVTRSQEE
360 370 380 390 400
ARRSHLARQQ QEKETQIVSL PQEENEVKSS QSLEEKSQSP SPPPLPEDLE
410 420 430 440 450
KAPVVLQPEQ IVSEEETPPP LLTKEASSPP THIQLQEEME PVEGPAPPVL
460 470 480 490 500
IQLSPPNTDA GAREPLASPH PAQLLRSLSP LSGTTDTKAE SPAGRVSDES
510 520 530 540 550
VLPLAQKSSL PECSTQKGVE SEREKSAPLP LTVEEFAPAK GITEEPMKKQ
560 570 580 590 600
SLEQKEGRRA SHALFPEHSG KQSADSSSSR SSSPSSSSSP SRSPSPDSVA
610 620 630 640 650
SRPQSSPGSK QRDGAQARVH ANPHERPKMG SRSTSESRSR SRSRSRSASS
660 670 680 690 700
SSRKSLSPGV SRDSNTSYTE TKDPSCGQEA AAPSGPQLQV LEPKEKAPTF
710 720 730 740 750
SASVRGRHLS HPEPEQQHVI QRLQPEQGSP KKCEAEEAEP PAATQPQTSE
760 770 780 790 800
TQISHLLESE RTHHTVEEKE EVTMDTSENR PENEVPEPPL PVADQVSNDE
810 820 830 840 850
RPEGGAEEEE KKESSMPKSF KRKISVVSAT KGVQAGNSDT EGGQPGRKRR
860 870 880 890 900
WGASTAATQK KPSISITTES LKSLIPDIKP LAGQEAVVDL HADDSRISED
910 920 930 940 950
ETERNGDDGT HDKGLKICRT VTQVVPAEGQ ENGQREEEEE KEPEAELPAP
960 970 980 990 1000
PQVSVEVALP PPVEHEVKKV TLGDTLTRRS ISQQKSGVSI TIDDPVRTAQ
1010 1020 1030 1040 1050
VPSPPRGKIS NIVHISNLVR PFTLGQLKEL LGRTGTLVEE AFWIDKIKSH
1060 1070 1080 1090 1100
CFVTYSTVEE AVATRTALHG VKWPQSNPKF LCADYAEQDE LDYHRGLLVD
1110 1120 1130 1140 1150
RPSETKAEEQ GAPRPLHPPP PPPVQPPPHP RAEQREQERA VREQWAERER
1160 1170 1180 1190 1200
EMERRERTRS EREWDRDKVR EGPRSRSRSR DRRRKERAKS KEKKSEKKEK
1210 1220 1230 1240 1250
AQEEPPAKLL DDLFRKTKAA PCIYWLPLTE SQIVQKEAEQ AERAKEREKR
1260 1270 1280 1290 1300
RKEREEEEQK EREKEAERER NRQLEREKRR EHSRERERDR ERERDRGDRE
1310 1320 1330 1338
RERERDRDRG RERDRRDTKR HSRSRSRSTP VRDRGGRR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1342 Collapse assay details

Data source: Panorama

Official Gene Symbol
ACIN1
Peptide Modified Sequence
KIS[+80.0]VVSATK
Modification Type
Phospho (ST)
Protein - Site of Modification
825
Peptide - Site of Modification
3
Peptide Start
823
Peptide End
831
CPTAC ID
CPTAC-1342
Peptide Molecular Mass
1,011.5366
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 7.1 5.8 4.6 4.8 10.5 11.3 8.6 12 12.2 15 15 15
y7-98 (1+) 6.2 4.1 8.7 7.5 10.9 14 9.7 11.6 16.5 15 15 15
y8-98 (1+) 6.8 5 8.6 7.8 11.3 13.7 10.3 12.4 16.2 15 15 15
sum 5.3 3.4 7 6.1 10.1 12.6 8.1 10.7 14.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3629 Collapse assay details

Data source: Panorama

Official Gene Symbol
Acin1
Peptide Sequence
GLLVDRPSETK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1096
Peptide End
1106
CPTAC ID
non-CPTAC-3629
Peptide Molecular Mass
1,213.6667
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (3+) 50.8 14.2 11.8 59.6 13 11.4 78.3 19.3 16.4 15 15 15
y7 (2+) 55.6 10 15.5 52 17.4 18 76.1 20.1 23.8 15 15 15
y8 (2+) 10.3 6.6 6.9 11.1 9 9.3 15.1 11.2 11.6 15 15 15
y9 (2+) 17.9 9.2 10.3 18.9 11.4 9.2 26 14.6 13.8 15 15 15
y2 (1+) 15.5 7.3 5.4 15.3 9.5 6.9 21.8 12 8.8 15 15 15
sum 7.4 4.6 5.4 10.7 6.8 7.1 13 8.2 8.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments